LOCUS       V008329                 8584 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008329
VERSION     V008329
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 8584)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8584)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 45899
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      656..823
                     /note="ori; derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC"
                     /label="RepC"
                     /note="polypeptide A"
                     /protein_id="AMR43707.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label="E149D"
                     /note="E149D"
     misc_feature    1794..2089
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     misc_feature    2408..2467
                     /label="leader peptide"
                     /note="leader peptide"
     CDS             2527..3258
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        3626..3730
                     /label="AmpR promoter"
     CDS             3731..4588
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4762..5350
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5702..6067
                     /gene="cadC"
                     /label="Cadmium resistance transcriptional regulatory
                     protein CadC"
                     /note="Cadmium resistance transcriptional regulatory
                     protein CadC from Staphylococcus aureus. Accession#:
                     P20047"
     misc_feature    6073..6117
                     /note="polylinker; derived from M13mp19"
     misc_feature    6129..8272
                     /note="luxAB; luciferase; derived from X06758"
     CDS             6151..7215
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA"
                     /label="luxA"
                     /protein_id="AMR43708.1"
                     /translation="MKFGNICFSYQPPGETHKQVMDRFVRLGIASEEVGFDTYWTLEHH
                     FTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFNFG
                     TVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYPKVY
                     SKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDISKID
                     HCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQWRDFVL
                     QGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDEIIASMR
                     RFMTQVAPFLKEPK"
     gene            6151..7215
                     /gene="luxA"
                     /label="luxA"
     CDS             7254..8234
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB"
                     /label="luxB"
                     /protein_id="AMR43709.1"
                     /translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVNE
                     HHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGRFI
                     LGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSINPH
                     CYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYGIDIS
                     DVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVGSHDDY
                     YESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP"
     gene            7254..8234
                     /gene="luxB"
                     /label="luxB"
     misc_feature    8281..8580
                     /label="blaZTT"
                     /note="blaZTT"
     misc_feature    8443..8580
                     /label="TT"
                     /note="TT"
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 aattgaatga gacatgctac acctccggat aataaatata tataaacgta tatagatttc
     2161 ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac cgtgtgctct
     2221 acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa agaaattaga
     2281 taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc
     2341 actcatcatg ttcatattta tcagagctcg tgctataatt atactaattt tataaggagg
     2401 aaaaaatatg ggcattttta gtatttttgt aatcagcaca gttcattatc aaccaaacaa
     2461 aaaataagtg gttataatga atcgttaata agcaaaattc atataaccaa attaaagagg
     2521 gttataatga acgagaaaaa tataaaacac agtcaaaact ttattacttc aaaacataat
     2581 atagataaaa taatgacaaa tataagatta aatgaacatg ataatatctt tgaaatcggc
     2641 tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt aactgccatt
     2701 gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca cgataatttc
     2761 caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc ctataaaata
     2821 tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt ttttgatagt
     2881 atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt attaaataca
     2941 aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt aagtatggtt
     3001 ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag attaagtaga
     3061 aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt tatgaaatgg
     3121 gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     3181 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     3241 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
     3301 cgtgtgccta ttttttgtga atcgattatg tcttttgcgc agtcggctta aaccagtttt
     3361 ccgcggcgct cgagcggccg catagttaag ccagccccga cacccgccaa cacccgctga
     3421 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3481 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4561 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5461 tgagtgagct ggcggccgct gcatgcgcac ttattcaagt gtatttttta ataaattatt
     5521 ttacttattg aaatgtatta ttttctaatg tcataccctg gtcaaaaccg ttcgtttttg
     5581 agactagaat tttatgccct acttacttct tttattttca ttcaaatatt tgcttgcatg
     5641 atgagtcgaa aatggttata atacactcaa ataaatattt gaatgaagat gggatgataa
     5701 tatgaaaaag aaagatactt gtgaaatttt ttgttatgac gaagaaaagg ttaatcgaat
     5761 acaaggggat ttacaaacag ttgatatttc tggtgttagc caaattttaa aggctattgc
     5821 cgatgaaaat agagcaaaaa ttacttacgc tctgtgtcag gatgaagagt tgtgtgtttg
     5881 tgatatagca aatatcttag gtgttacgat agcaaatgca tctcatcatt tacgtacgct
     5941 ttataagcaa ggggtggtca actttagaaa agaaggaaaa ctagctttat attctttagg
     6001 tgatgaacat atcaggcaga taatgatgat cgccctagca cataagaaag aagtgaaggt
     6061 caatgtctga acctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcgtt
     6121 aactaattaa tcaccaaaaa ggaatagagt atgaagtttg gaaatatttg tttttcgtat
     6181 caaccaccag gtgaaactca taagcaagta atggatcgct ttgttcggct tggtatcgcc
     6241 tcagaagagg tagggtttga tacatattgg accttagaac atcattttac agagtttggt
     6301 cttacgggaa atttatttgt tgctgcggct aacctgttag gaagaactaa aacattaaat
     6361 gttggcacta tgggggttgt tattccgaca gcacacccag ttcgacagtt agaagacgtt
     6421 ttattattag atcaaatgtc gaaaggtcgt tttaattttg gaaccgttcg agggctatac
     6481 cataaagatt ttcgagtatt tggtgttgat atggaagagt ctcgagcaat tactcaaaat
     6541 ttctaccaga tgataatgga aagcttacag acaggaacca ttagctctga tagtgattac
     6601 attcaatttc ctaaggttga tgtatatccc aaagtgtact caaaaaatgt accaacctgt
     6661 atgactgctg agtccgcaag tacgacagaa tggctagcaa tacaagggct accaatggtt
     6721 cttagttgga ttattggtac taatgaaaaa aaagcacaga tggaactcta taatgaaatt
     6781 gcgacagaat atggtcatga tatatctaaa atagatcatt gtatgactta tatttgttct
     6841 gttgatgatg atgcacaaaa ggcgcaagat gtttgtcggg agtttctgaa aaattggtat
     6901 gactcatatg taaatgcgac caatatcttt aatgatagca atcaaactcg tggttatgat
     6961 tatcataaag gtcaatggcg tgattttgtt ttacaaggac atacaaacac caatcgacgt
     7021 gttgattata gcaatggtat taaccctgta ggcactcctg agcagtgtat tgaaatcatt
     7081 caacgtgata ttgatgcaac gggtattaca aacattacat gcggatttga agctaatgga
     7141 actgaagatg aaataattgc ttccatgcga cgctttatga cacaagtcgc tcctttctta
     7201 aaagaaccta aataaattac ttatttgata ctagagataa taaggaacaa gttatgaaat
     7261 ttggattatt ttttctaaac tttcagaaag atggaataac atctgaagaa acgttggata
     7321 atatggtaaa gactgtcacg ttaattgatt caactaaata tcattttaat actgcctttg
     7381 ttaatgaaca tcacttttca aaaaatggta ttgttggagc acctattacc gcagctggtt
     7441 ttttattagg gttaacaaat aaattacata ttggttcatt aaatcaagta attaccaccc
     7501 atcaccctgt acgtgtagca gaagaagcca gtttattaga tcaaatgtca gagggacgct
     7561 tcattcttgg ttttagtgac tgcgaaagtg atttcgaaat ggaatttttt agacgtcata
     7621 tctcatcaag gcaacaacaa tttgaagcat gctatgaaat aattaatgac gcattaacta
     7681 caggttattg tcatccccaa aacgactttt atgattttcc aaaggtttca attaatccac
     7741 actgttacag tgagaatgga cctaagcaat atgtatccgc tacatcaaaa gaagtcgtca
     7801 tgtgggcagc gaaaaaggca ctgcctttaa catttaagtg ggaggataat ttagaaacca
     7861 aagaacgcta tgcaattcta tataataaaa cagcacaaca atatggtatt gatatttcgg
     7921 atgttgatca tcaattaact gtaattgcga acttaaatgc tgatagaagt acggctcaag
     7981 aagaagtgag agaatactta aaagactata tcactgaaac ttaccctcaa atggacagag
     8041 atgaaaaaat taactgcatt attgaagaga atgcagttgg gtctcatgat gactattatg
     8101 aatcgacaaa attagcagtg gaaaaaacag ggtctaaaaa tattttatta tcctttgaat
     8161 caatgtccga tattaaagat gtaaaagata ttattgatat gttgaaccaa aaaatcgaaa
     8221 tgaatttacc ataataaaat taaaggcaat ttctatatta gattgccttt ttggcgcgcc
     8281 tattctaatg cataataaat actgataaca tcttatattt tgtattatat tttgtattat
     8341 cgttgacatg tataattttg atatcaaaaa ctgattttcc ctctattatt ttcgagattt
     8401 attttcttaa ttctctttaa caaactagaa atattgtata tacaaaaaat tataaataat
     8461 agatgaatag tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag
     8521 tgcgttgctt ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca
     8581 ggcg
//
