LOCUS       V008330                 7999 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008330
VERSION     V008330
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 7999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 47381
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      656..823
                     /note="ori; derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC protein"
                     /label="RepC protein"
                     /note="polypeptide A"
                     /protein_id="AMR43704.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label="E149D"
                     /note="E149D"
     misc_feature    1794..2089
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     misc_feature    2408..2467
                     /label="leader peptide"
                     /note="leader peptide"
     CDS             2527..3258
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        3626..3730
                     /label="AmpR promoter"
     CDS             3731..4588
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4762..5350
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5487..5531
                     /note="polylinker; derived from M13mp19"
     misc_feature    5543..7686
                     /note="luxAB; luciferase; derived from X06758"
     CDS             5565..6629
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA"
                     /label="luxA"
                     /note="luciferase"
                     /protein_id="AMR43702.1"
                     /translation="MKFGNICFSYQPPGETHKQVMDRFVRLGIASEEVGFDTYWTLEHH
                     FTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFNFG
                     TVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYPKVY
                     SKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDISKID
                     HCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQWRDFVL
                     QGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDEIIASMR
                     RFMTQVAPFLKEPK"
     gene            5565..6629
                     /gene="luxA"
                     /label="luxA"
     CDS             6668..7648
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB"
                     /label="luxB"
                     /note="luciferase"
                     /protein_id="AMR43701.1"
                     /translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVNE
                     HHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGRFI
                     LGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSINPH
                     CYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYGIDIS
                     DVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVGSHDDY
                     YESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP"
     gene            6668..7648
                     /gene="luxB"
                     /label="luxB"
     misc_feature    7695..7995
                     /label="blaZTT"
                     /note="blaZTT"
     misc_feature    7858..7995
                     /label="TT"
                     /note="TT"
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 aattgaatga gacatgctac acctccggat aataaatata tataaacgta tatagatttc
     2161 ataaagtcta acacactaga cttatttact tcgtaattaa gtcgttaaac cgtgtgctct
     2221 acgaccaaaa ctataaaacc tttaagaact ttcttttttt acaagaaaaa agaaattaga
     2281 taaatctctc atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc
     2341 actcatcatg ttcatattta tcagagctcg tgctataatt atactaattt tataaggagg
     2401 aaaaaatatg ggcattttta gtatttttgt aatcagcaca gttcattatc aaccaaacaa
     2461 aaaataagtg gttataatga atcgttaata agcaaaattc atataaccaa attaaagagg
     2521 gttataatga acgagaaaaa tataaaacac agtcaaaact ttattacttc aaaacataat
     2581 atagataaaa taatgacaaa tataagatta aatgaacatg ataatatctt tgaaatcggc
     2641 tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt aactgccatt
     2701 gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca cgataatttc
     2761 caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc ctataaaata
     2821 tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt ttttgatagt
     2881 atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt attaaataca
     2941 aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt aagtatggtt
     3001 ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag attaagtaga
     3061 aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt tatgaaatgg
     3121 gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     3181 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     3241 tataaattat ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt
     3301 cgtgtgccta ttttttgtga atcgattatg tcttttgcgc agtcggctta aaccagtttt
     3361 ccgcggcgct cgagcggccg catagttaag ccagccccga cacccgccaa cacccgctga
     3421 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3481 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3961 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4021 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4081 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4141 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4201 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4261 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4321 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4381 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4441 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4501 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4561 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5461 tgagtgagct ggcggccgct gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
     5521 agctcgaatt cgttaactaa ttaatcacca aaaaggaata gagtatgaag tttggaaata
     5581 tttgtttttc gtatcaacca ccaggtgaaa ctcataagca agtaatggat cgctttgttc
     5641 ggcttggtat cgcctcagaa gaggtagggt ttgatacata ttggacctta gaacatcatt
     5701 ttacagagtt tggtcttacg ggaaatttat ttgttgctgc ggctaacctg ttaggaagaa
     5761 ctaaaacatt aaatgttggc actatggggg ttgttattcc gacagcacac ccagttcgac
     5821 agttagaaga cgttttatta ttagatcaaa tgtcgaaagg tcgttttaat tttggaaccg
     5881 ttcgagggct ataccataaa gattttcgag tatttggtgt tgatatggaa gagtctcgag
     5941 caattactca aaatttctac cagatgataa tggaaagctt acagacagga accattagct
     6001 ctgatagtga ttacattcaa tttcctaagg ttgatgtata tcccaaagtg tactcaaaaa
     6061 atgtaccaac ctgtatgact gctgagtccg caagtacgac agaatggcta gcaatacaag
     6121 ggctaccaat ggttcttagt tggattattg gtactaatga aaaaaaagca cagatggaac
     6181 tctataatga aattgcgaca gaatatggtc atgatatatc taaaatagat cattgtatga
     6241 cttatatttg ttctgttgat gatgatgcac aaaaggcgca agatgtttgt cgggagtttc
     6301 tgaaaaattg gtatgactca tatgtaaatg cgaccaatat ctttaatgat agcaatcaaa
     6361 ctcgtggtta tgattatcat aaaggtcaat ggcgtgattt tgttttacaa ggacatacaa
     6421 acaccaatcg acgtgttgat tatagcaatg gtattaaccc tgtaggcact cctgagcagt
     6481 gtattgaaat cattcaacgt gatattgatg caacgggtat tacaaacatt acatgcggat
     6541 ttgaagctaa tggaactgaa gatgaaataa ttgcttccat gcgacgcttt atgacacaag
     6601 tcgctccttt cttaaaagaa cctaaataaa ttacttattt gatactagag ataataagga
     6661 acaagttatg aaatttggat tattttttct aaactttcag aaagatggaa taacatctga
     6721 agaaacgttg gataatatgg taaagactgt cacgttaatt gattcaacta aatatcattt
     6781 taatactgcc tttgttaatg aacatcactt ttcaaaaaat ggtattgttg gagcacctat
     6841 taccgcagct ggttttttat tagggttaac aaataaatta catattggtt cattaaatca
     6901 agtaattacc acccatcacc ctgtacgtgt agcagaagaa gccagtttat tagatcaaat
     6961 gtcagaggga cgcttcattc ttggttttag tgactgcgaa agtgatttcg aaatggaatt
     7021 ttttagacgt catatctcat caaggcaaca acaatttgaa gcatgctatg aaataattaa
     7081 tgacgcatta actacaggtt attgtcatcc ccaaaacgac ttttatgatt ttccaaaggt
     7141 ttcaattaat ccacactgtt acagtgagaa tggacctaag caatatgtat ccgctacatc
     7201 aaaagaagtc gtcatgtggg cagcgaaaaa ggcactgcct ttaacattta agtgggagga
     7261 taatttagaa accaaagaac gctatgcaat tctatataat aaaacagcac aacaatatgg
     7321 tattgatatt tcggatgttg atcatcaatt aactgtaatt gcgaacttaa atgctgatag
     7381 aagtacggct caagaagaag tgagagaata cttaaaagac tatatcactg aaacttaccc
     7441 tcaaatggac agagatgaaa aaattaactg cattattgaa gagaatgcag ttgggtctca
     7501 tgatgactat tatgaatcga caaaattagc agtggaaaaa acagggtcta aaaatatttt
     7561 attatccttt gaatcaatgt ccgatattaa agatgtaaaa gatattattg atatgttgaa
     7621 ccaaaaaatc gaaatgaatt taccataata aaattaaagg caatttctat attagattgc
     7681 ctttttggcg cgcctattct aaatgcataa taaatactga taacatctta tattttgtat
     7741 tatattttgt attatcgttg acatgtataa ttttgatatc aaaaactgat tttccctcta
     7801 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
     7861 aaaattataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
     7921 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
     7981 tcaagcaaag tgacaggcg
//
