LOCUS       V008331                 6592 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008331
VERSION     V008331
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 6592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 38389
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2087
                     /note="T181cop-623 repC; Staphylococcus replication
                     cassette; high-copy-number variant; derived from J04764"
     misc_feature    158..391
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      654..821
                     /note="ori; derived from Staphylococcus aureus"
     CDS             686..1630
                     /codon_start=1
                     /product="RepC protein"
                     /label="RepC protein"
                     /note="polypeptide A"
                     /protein_id="AMR43698.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1130..1132
                     /label="E149D"
                     /note="E149D"
     misc_feature    1792..2087
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     misc_feature    2406..2465
                     /label="leader peptide"
                     /note="leader peptide"
     CDS             2525..3256
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     promoter        3624..3728
                     /label="AmpR promoter"
     CDS             3729..4586
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4760..5348
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5485..5529
                     /note="polylinker; derived from M13mp19"
     CDS             5563..6276
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    6288..6588
                     /label="blaZTT"
                     /note="blaZTT"
     misc_feature    6451..6588
                     /label="TT"
                     /note="TT"
ORIGIN
        1 cctttgcgaa agagttaata agttaacaga agatgaacca aaactaaatg gtttagcagg
       61 aaacttagat aaaaaaatga atccagaatt atattcagaa caggaacagc aacaagaaca
      121 acaaaagaat caaaaacgag atagaggtat gcacttatag aacatgcatt tatgccgaga
      181 aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt ggttggactt
      241 gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc tgtaggttcc
      301 gaccaataag gaaattggaa taaagcaata aaaggagttg aagaaatgaa attcagagaa
      361 gcctttgaga attttataac aagtaagtat gtacttggtg ttttagtagt tttaactgtt
      421 taccagataa tacaaatgct taaataaaaa aagacttgat ctgattagac caaatctttt
      481 gatagtgtta tattaataac aaaataaaaa ggagtcgctc acgccctacc aaagtttgtg
      541 aacgacatca ttcaaagaaa aaaacactga gttgttttta taatcttgta tatttagata
      601 ttaaacgata tttaaatata catcaagata tatatttggg tgagcgatta cttaaacgaa
      661 attgagatta aggagtcgat tttttatgta taaaaacaat catgcaaatc attcaaatca
      721 tttggaaaat cacgatttag acaatttttc taaaaccggc tactctaata gccggttgga
      781 cgcacatact gtgtgcatat ctgatccaaa attaagtttt gatgcaatga cgatcgttgg
      841 aaatctcaac cgagacaacg ctcaagccct ttctaaattt atgagtgtag agccccaaat
      901 aagactttgg gatattcttc aaacaaagtt taaagctaaa gcacttcaag aaaaagttta
      961 tattgaatat gacaaagtga aagcagatag ttgggataga cgtaatatgc gtattgaatt
     1021 taatccaaac aaacttacac gagatgaaat gatttggtta aaacaaaata taataagcta
     1081 catggaagat gacggtttta caagattaga tttagccttt gattttgaag atgatttgag
     1141 tgactactat gcaatgtctg ataaagcagt taagaaaact attttttatg gtcgtaatgg
     1201 taagccagaa acaaaatatt ttggcgtgag agatagtaat agatttatta gaatttataa
     1261 taaaaagcaa gaacgtaaag ataatgcaga tgctgaagtt atgtctgaac atttatggcg
     1321 tgtagaaatc gaacttaaaa gagatatggt ggattactgg aatgattgct ttagtgattt
     1381 acatatcttg caaccagatt ggaaaactat ccaacgcact gcggatagag caatagtttt
     1441 tatgttattg agtgatgaag aagaatgggg aaagcttcac agaaattcta gaacaaaata
     1501 taagaatttg ataaaagaaa tttcgccagt cgatttaacg gacttaatga aatcgacttt
     1561 aaaagcgaac gaaaaacaat tgcaaaaaca aatcgatttt tggcaacatg aatttaaatt
     1621 ttggaaatag tgtacatatt aatattactg aacaaaaatg atatatttaa actattctaa
     1681 tttaggagga tttttttatg aagtgtctat ttaaaaattt ggggaattta tatgaggtga
     1741 aagaataatt tacccctata aactttagtc acctcaagta aagaggtaaa attgtttagt
     1801 ttatataaaa aatttaaagg tttgttttat agcgttttat tttggctttg tattctttca
     1861 ttttttagtg tattaaatga aatggtttta aatgtttctt tacctgatat tgcaaatcat
     1921 tttaatacta ctcctggaat tacaaactgg gtaaacactg catatatgtt aactttttcg
     1981 ataggaacag cagtatatgg aaaattatct gattatataa atataaaaaa attgttaatt
     2041 attggtatta gtttgagctg tcttggttca ttgattgctt ttattgggcc cacctaggaa
     2101 ttgaatgaga catgctacac ctccggataa taaatatata taaacgtata tagatttcat
     2161 aaagtctaac acactagact tatttacttc gtaattaagt cgttaaaccg tgtgctctac
     2221 gaccaaaact ataaaacctt taagaacttt ctttttttac aagaaaaaag aaattagata
     2281 aatctctcat atcttttatt caataatcgc atccgattgc agtataaatt taacgatcac
     2341 tcatcatgtt catatttatc agagctcgtg ctataattat actaatttta taaggaggaa
     2401 aaaatatggg catttttagt atttttgtaa tcagcacagt tcattatcaa ccaaacaaaa
     2461 aataagtggt tataatgaat cgttaataag caaaattcat ataaccaaat taaagagggt
     2521 tataatgaac gagaaaaata taaaacacag tcaaaacttt attacttcaa aacataatat
     2581 agataaaata atgacaaata taagattaaa tgaacatgat aatatctttg aaatcggctc
     2641 aggaaaaggc cattttaccc ttgaattagt aaagaggtgt aatttcgtaa ctgccattga
     2701 aatagaccat aaattatgca aaactacaga aaataaactt gttgatcacg ataatttcca
     2761 agttttaaac aaggatatat tgcagtttaa atttcctaaa aaccaatcct ataaaatata
     2821 tggtaatata ccttataaca taagtacgga tataatacgc aaaattgttt ttgatagtat
     2881 agctaatgag atttatttaa tcgtggaata cgggtttgct aaaagattat taaatacaaa
     2941 acgctcattg gcattacttt taatggcaga agttgatatt tctatattaa gtatggttcc
     3001 aagagaatat tttcatccta aacctaaagt gaatagctca cttatcagat taagtagaaa
     3061 aaaatcaaga atatcacaca aagataaaca aaagtataat tatttcgtta tgaaatgggt
     3121 taacaaagaa tacaagaaaa tatttacaaa aaatcaattt aacaattcct taaaacatgc
     3181 aggaattgac gatttaaaca atattagctt tgaacaattc ttatctcttt tcaatagcta
     3241 taaattattt aataagtaag ttaagggatg cataaactgc atcccttaac ttgtttttcg
     3301 tgtgcctatt ttttgtgaat cgattatgtc ttttgcgcag tcggcttaaa ccagttttcc
     3361 gcggcgctcg agcggccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     3421 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3481 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     3541 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5461 agtgagctgg cggccgctgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     5521 ctcgaattcg ttaactaatt aatttaagaa ggagatatac atatgagtaa aggagaagaa
     5581 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     5641 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     5701 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcgcgtat
     5761 ggtcttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     5821 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     5881 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     5941 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     6001 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     6061 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     6121 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     6181 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     6241 gctgggatta cacatggcat ggatgaacta tacaaataag gcgcgcctat tctaaatgca
     6301 taataaatac tgataacatc ttatattttg tattatattt tgtattatcg ttgacatgta
     6361 taattttgat atcaaaaact gattttccct ctattatttt cgagatttat tttcttaatt
     6421 ctctttaaca aactagaaat attgtatata caaaaaatta taaataatag atgaatagtt
     6481 taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt
     6541 ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg cg
//
