LOCUS       V008332                 5730 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008332
VERSION     V008332
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 5730)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 52846
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      656..823
                     /note="ori; derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC"
                     /label="RepC"
                     /note="polypeptide A"
                     /protein_id="AMR43712.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label="E149D"
                     /note="E149D"
     misc_feature    1794..2089
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     regulatory      2246..2251
                     /regulatory_class="minus_35_signal"
     regulatory      2269..2274
                     /regulatory_class="minus_10_signal"
     regulatory      2423..2428
                     /regulatory_class="ribosome_binding_site"
     CDS             2434..3213
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     regulatory      3292..3338
                     /regulatory_class="terminator"
     repeat_region   3292..3309
                     /label="inverted repeat"
                     /note="inverted repeat"
     repeat_region   3319..3338
                     /label="inverted repeat"
                     /note="inverted repeat"
     promoter        3666..3770
                     /label="AmpR promoter"
     CDS             3771..4628
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4802..5390
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5527..5571
                     /note="polylinker; derived from M13mp19"
     primer_bind     complement(5572..5588)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 atcgaatccc ttcgtgagca tcaaataaca gcaaatgtag tctcaaaacc caagaaaagt
     2161 tctcgttcgg aggaaatcct tattgaaacc ttaaaaagaa gagtaatgga attagaaaaa
     2221 gaaaataaaa aattacagaa ccaaattcaa aaattatatg gagatctgta taataaagaa
     2281 taattattaa tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt
     2341 attgaacagt aatttaagta tatgtccaat ctagggtaag taaattgagt atcaatataa
     2401 actttatatg aacataatca acgaggtgaa atcatgagca atttgattaa cggaaaaata
     2461 ccaaatcaag cgattcaaac attaaaaatc gtaaaagatt tatttggaag ttcaatagtt
     2521 ggagtatatc tatttggttc agcagtaaat ggtggtttac gcattaacag cgatgtagat
     2581 gttctagtcg tcgtgaatca tagtttacct caattaactc gaaaaaaact aacagaaaga
     2641 ctaatgacta tatcaggaaa gattggaaat acggattctg ttagaccact tgaagttacg
     2701 gttataaata ggagtgaagt tgtcccttgg caatatcctc caaaaagaga atttatatac
     2761 ggtgagtggc tcaggggtga atttgagaat ggacaaattc aggaaccaag ctatgatcct
     2821 gatttggcta ttgttttagc acaagcaaga aagaatagta tttctctatt tggtcctgat
     2881 tcttcaagta tacttgtctc cgtacctttg acagatattc gaagagcaat taaggattct
     2941 ttgccagaac taattgaggg gataaaaggt gatgagcgta atgtaatttt aaccctagct
     3001 cgaatgtggc aaacagtgac tactggtgaa attacctcga aagatgtcgc tgcagaatgg
     3061 gctatacctc ttttacctaa agagcatgta actttactgg atatagctag aaaaggctat
     3121 cggggagagt gtgatgataa gtgggaagga ctatattcaa aggtgaaagc actcgttaag
     3181 tatatgaaaa attctataga aacttctctc aattaggcta attttattgc aataacaggt
     3241 gcttactttt aaaactactg atttattgat aaatattgaa caatttttgg gaagaataaa
     3301 gcgtcctctt gtgaaattag agaacgcttt attactttaa tttagtgaaa caatttgtaa
     3361 ctattgaaaa tagaaagaaa ttgttccttg atagtttatt accgcggcct cgagcggccg
     3421 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3481 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3541 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3601 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3661 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3721 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3781 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3841 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3901 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3961 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4021 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4081 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4141 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4201 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4261 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4321 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4381 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4441 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4501 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4561 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4621 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4681 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4741 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4801 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4861 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4921 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4981 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5041 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5101 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5161 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5221 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5281 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5341 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5401 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5461 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct ggcggccgct
     5521 gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt
     5581 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5641 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5701 acagttgcgc agcctgaatg gcgaatggcg
//
