LOCUS       V008341                 5426 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008341
VERSION     V008341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Sequencing of a set of cassette-based shuttle vectors for
            Gram-positive bacteria deposited at BEI resources from the NARSA
            collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Riojas MA, Bojja K, Byrd R, Hamid S, Mooney S, Monaco A, Alalade R,
            King S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 5426)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 15854
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-MAY-2015) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..2089
                     /note="pT181copwt repC; Staphylococcus replication
                     cassette; derived from J04764"
     misc_feature    159..392
                     /label="RS-B site"
                     /note="RS-B site"
     rep_origin      656..823
                     /note="ori; derived from Staphylococcus aureus"
     CDS             688..1632
                     /codon_start=1
                     /product="RepC protein"
                     /label="RepC protein"
                     /note="polypeptide A"
                     /protein_id="AMR43720.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     variation       1132..1134
                     /label="E149D"
                     /note="E149D"
     misc_feature    1794..2089
                     /label="truncated polypeptide B"
                     /note="truncated polypeptide B"
     CDS             2372..3019
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     promoter        3362..3466
                     /label="AmpR promoter"
     CDS             3467..4324
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4498..5086
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    5223..5267
                     /note="polylinker; derived from M13mp19"
     primer_bind     complement(5268..5284)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctttgcgga aagagttaat aagttaacag aagatgaacc aaaactaaat ggtttagcag
       61 gaaacttaga taaaaaaatg aatccagaat tatattcaga acaggaacag caacaagaac
      121 aacaaaagaa tcaaaaacga gatagaggta tgcacttata gaacatgcat ttatgccgag
      181 aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact
      241 tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc
      301 cgaccaataa ggaaattgga ataaagcaat aaaaggagtt gaagaaatga aattcagaga
      361 agcctttgag aattttataa caagtaagta tgtacttggt gttttagtag ttttaactgt
      421 ttaccagata atacaaatgc ttaaataaaa aaagacttga tctgattaga ccaaatcttt
      481 tgatagtgtt atattaataa caaaataaaa aggagtcgct cacgccctga ccaaagtttg
      541 tgaacgacat cattcaaaga aaaaaacact gagttgtttt tataatcttg tatatttaga
      601 tattaaacga tatttaaata tacatcaaga tatatatttg ggtgagcgat tccttaaacg
      661 aaattgagat taaggagtcg attttttatg tataaaaaca atcatgcaaa tcattcaaat
      721 catttggaaa atcacgattt agacaatttt tctaaaaccg gctactctaa tagccggttg
      781 gacgcacata ctgtgtgcat atctgatcca aaattaagtt ttgatgcaat gacgatcgtt
      841 ggaaatctca accgagacaa cgctcaagcc ctttctaaat ttatgagtgt agagccccaa
      901 ataagacttt gggatattct tcaaacaaag tttaaagcta aagcacttca agaaaaagtt
      961 tatattgaat atgacaaagt gaaagcagat agttgggata gacgtaatat gcgtattgaa
     1021 tttaatccaa acaaacttac acgagatgaa atgatttggt taaaacaaaa tataataagc
     1081 tacatggaag atgacggttt tacaagatta gatttagcct ttgattttga agatgatttg
     1141 agtgactact atgcaatgtc tgataaagca gttaagaaaa ctatttttta tggtcgtaat
     1201 ggtaagccag aaacaaaata ttttggcgtg agagatagta atagatttat tagaatttat
     1261 aataaaaagc aagaacgtaa agataatgca gatgctgaag ttatgtctga acatttatgg
     1321 cgtgtagaaa tcgaacttaa aagagatatg gtggattact ggaatgattg ctttagtgat
     1381 ttacatatct tgcaaccaga ttggaaaact atccaacgca ctgcggatag agcaatagtt
     1441 tttatgttat tgagtgatga agaagaatgg ggaaagcttc acagaaattc tagaacaaaa
     1501 tataagaatt tgataaaaga aatttcgcca gtcgatttaa cggacttaat gaaatcgact
     1561 ttaaaagcga acgaaaaaca attgcaaaaa caaatcgatt tttggcaaca tgaatttaaa
     1621 ttttggaaat agtgtacata ttaatattac tgaacaaaaa tgatatattt aaactattct
     1681 aatttaggag gattttttta tgaagtgtct atttaaaaat ttggggaatt tatatgaggt
     1741 gaaagaataa tttaccccta taaactttag tcacctcaag taaagaggta aaattgttta
     1801 gtttatataa aaaatttaaa ggtttgtttt atagcgtttt attttggctt tgtattcttt
     1861 cattttttag tgtattaaat gaaatggttt taaatgtttc tttacctgat attgcaaatc
     1921 attttaatac tactcctgga attacaaact gggtaaacac tgcatatatg ttaacttttt
     1981 cgataggaac agcagtatat ggaaaattat ctgattatat aaatataaaa aaattgttaa
     2041 ttattggtat tagtttgagc tgtcttggtt cattgattgc ttttattggg cccacctagg
     2101 tattatcaag ataagaaaga aaaggatttt tcgctacgct caaatccttt aaaaaaacac
     2161 aaaagaccac attttttaat gtggtctttt attcttcaac taaagcaccc attagttcaa
     2221 caaacgaaaa ttggataaag tgggatattt ttaaaatata tatttatgtt acagtaatat
     2281 tgacttttaa aaaaggattg attctaatga agaaagcaga caagtaagcc tcctaaattc
     2341 actttagata aaaatttagg aggcatatca aatgaacttt aataaaattg atttagacaa
     2401 ttggaagaga aaagagatat ttaatcatta tttgaaccaa caaacgactt ttagtataac
     2461 cacagaaatt gatattagtg ttttataccg aaacataaaa caagaaggat ataaatttta
     2521 ccctgcattt attttcttag tgacaagggt gataaactca aatacagctt ttagaactgg
     2581 ttacaatagc gacggagagt taggttattg ggataagtta gagccacttt atacaatttt
     2641 tgatggtgta tctaaaacat tctctggtat ttggactcct gtaaagaatg acttcaaaga
     2701 gttttatgat ttataccttt ctgatgtaga gaaatataat ggttcgggga aattgtttcc
     2761 caaaacacct atacctgaaa atgctttttc tctttctatt attccatgga cttcatttac
     2821 tgggtttaac ttaaatatca ataataatag taattacctt ctacccatta ttacagcagg
     2881 aaaattcatt aataaaggta attcaatata tttaccgcta tctttacagg tacatcattc
     2941 tgtttgtgat ggttatcatg caggattgtt tatgaactct attcaggaat tgtcagatag
     3001 gcctaatgac tggcttttat aatatgagat aatgccgact gtacttttta cagtcggttt
     3061 tctaatgtca ctaacctgcc ccgttagttg aagaaggccg cggcctcgag cggccgcata
     3121 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3181 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3241 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     3361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctggcg gccgctgcat
     5221 gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact ggccgtcgtt
     5281 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5341 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5401 ttgcgcagcc tgaatggcga atggcg
//
