LOCUS       40924_12485        6003 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCN-MET3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack 
            BP.
  TITLE     Expression Plasmids for Use in Candida glabrata
  JOURNAL   G3 (Bethesda) 3 (10), 1675-1686 (2013)
  PUBMED    23934995
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Zordan RE, Cormack BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns 
            Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, 
            Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 6003)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)"; date: "2013"; volume: "3"; issue: "10"; pages: 
            "1675-1686"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School 
            of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        182..585
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             605..1168
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     terminator      1178..1425
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     1625..1641
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1655..2680
                     /label=Candida glabrata MET3 promoter (BG2)
                     /note="Candida glabrata MET3 promoter (BG2)"
                     /regulatory_class="promoter"
     primer_bind     2681..2697
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2731..2747)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2750..3147
                     /label=region downstream of HIS3 in Candida glabrata BG2
                     /note="region downstream of HIS3 in Candida glabrata BG2"
                     /regulatory_class="terminator"
     primer_bind     complement(3184..3200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3208..3224)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3232..3262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3277..3298)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3586..4174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4348..5205)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5206..5310)
                     /label=AmpR promoter
     misc_feature    5342..5933
                     /label=Candida glabrata CEN/ARS
                     /note="Candida glabrata CEN/ARS"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagagacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa
      481 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
      541 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagttttg gatctaaaat
      601 gggcaccact cttgacgaca cggcttaccg ataccgcacc agtgtcccgg gggacgccga
      661 ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac
      721 cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc
      781 cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgaccctg actcccggac
      841 gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg
      901 ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg
      961 ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc cgcgagctgg gcgccgggca
     1021 cctctggctg gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg
     1081 gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca
     1141 ggcgctctac atgagcatgc cctgccccta agtcgagtca tgtaattagt tatgtcacgc
     1201 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     1261 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     1321 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     1381 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg tatgcggtgt
     1441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     1501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1561 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1621 cgttgtaaaa cgacggccag tgaattgaga gctcatacca gttacaatta gtattacaat
     1681 ggtttaccaa gtacaaatta tgtaatatgg ttataattgg cttatatgta tggaggtagg
     1741 gaactgcagg ttattgttct gttaaactac cagtaatgca agacggctaa gtcatatgac
     1801 tgccactttg tcaatcctga agaaaatgac aatagaaagt gatataatac acgtgatatg
     1861 cgtaggatgg gacccaaaga tagccacatc acatgatctt caaaaacccc gtcaaaaagg
     1921 aataaatcga aggaaaaaaa atgccacgtg actttgatag tcaaaatcag gttatattac
     1981 tatacgatcc cccacacata acctttacca cacaccgcac gggctggact tatcctatta
     2041 gaaagcggtg cagccagggc caagaaaacg cgaacgacgc agaaaaaacg tcaagcaaaa
     2101 aaactgtggt gttatttaaa gcatacaatt tcctgcctcc ctttaatgct acgggggatc
     2161 tagcaaatgg gaaaatatca catgactttg gctagaaggg gtgggaaaac agatatttta
     2221 gtcacattgt ttgatttcac gtactacacg atacactaca ctacacaata cactgcaata
     2281 cactacactg tatggttccc tccggccctg gatatgctag cgaaggatcc caagcccatc
     2341 gaggaaatca ttcaagagca tctgcaggtc tcaaatacac taagtccaaa cacaaacagc
     2401 actgagctaa tccaagacac caattgcatg ccttctcaat tagaacagtt tattacacag
     2461 cttataactg tgcatcttgc cattcttttc gagatagcca tttgcatttg attaacatgc
     2521 ttagctcgtt ctcaagccac agtaaaatgg attgcctttt gagtttccat cgttgtataa
     2581 ataggcactc attcctatag ccttgctctt tggtcttgtc tctgtaatgg taatagctgt
     2641 aagtcaggat ataacactcc agaaaagaaa cacctaacaa tctagaacta gtggatcccc
     2701 cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgaga acacaaccca
     2761 cagctaccac catcaacaat atttatatat ataacgtaca catagaaatc acacaaacag
     2821 agtatttatt cttaactaca tgaactacca tcagaccgtc tgggccacta tataatgtgc
     2881 cattcataaa cgtgatcact ttacgtagca ggcaacccca ggtgaaaatt tttcagcgag
     2941 ctgccagatt gtcaggtgaa aaactgaaaa aaacttctgg gcgatgagct tgtggcggga
     3001 aattaagtat ataaagcagt tagtttcttc tgcttcttgt ggttctgggt attcttgtaa
     3061 tatttggaag aatagaaaat caagactact gcctttcttt tcatattata gaggaagaaa
     3121 tacgcacgaa cacgatatag aggtaaaggt accttgcagg catgcaagct tggcgtaatc
     3181 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3241 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3301 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3361 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3421 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3481 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3541 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3601 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3661 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3721 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3781 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3841 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3901 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3961 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4021 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4081 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4141 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4201 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4261 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4321 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4381 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4441 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4501 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4561 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4621 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4681 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4741 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4801 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4861 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4921 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4981 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5041 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5101 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5161 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5221 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5281 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5341 cgggatctct aatgatgcaa tttttgtttt gtacactaag attctatttc ggaactctta
     5401 cccatgtcat tatttacttt attttcatta ttgtaatttt ttaagctttt attatgaatt
     5461 atttaaatac aattcttttt gatcatgtga cagacatcac tgaaagatgt gctctgcgtt
     5521 attagtcaga ttgaaatata cattttcatg tagatatgat gttcttactt taaatgttgt
     5581 tccatttgct agcttaaata gacgatatta taaatttaag tattcactaa aaatctgacc
     5641 cattaatgtt agaattagta ttattttttg tgatattaca tttttatatt ttggatttca
     5701 accaatgctc tgcgatatga ttcaacagaa taatatgtac aagctacttt aattcatata
     5761 tactaataga ttcacagatc cagtacgact gcaagaattt acgttttgtt tgaaatggta
     5821 gtagctacac taaattaaca atgatcgatt aatccacatc cacagtgtgt aatcatccta
     5881 atgaaaacaa gaataactac aaaaatgaat ataaactttt tgcatgttag tctgacgtct
     5941 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     6001 gtc
//
