LOCUS       40924_12460        6561 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCN-HHT2-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack 
            BP.
  TITLE     Expression Plasmids for Use in Candida glabrata
  JOURNAL   G3 (Bethesda) 3 (10), 1675-1686 (2013)
  PUBMED    23934995
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Zordan RE, Cormack BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns 
            Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, 
            Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 6561)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6561)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)"; date: "2013"; volume: "3"; issue: "10"; pages: 
            "1675-1686"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School 
            of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        182..585
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             605..1168
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     terminator      1178..1425
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     1625..1641
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1654..2515
                     /label=Candida glabrata HHT2 promoter
                     /note="Candida glabrata HHT2 promoter"
                     /regulatory_class="promoter"
     primer_bind     2516..2532
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2552..3265
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      3287..3684
                     /label=region downstream of HIS3 in Candida glabrata BG2
                     /note="region downstream of HIS3 in Candida glabrata BG2"
                     /regulatory_class="terminator"
     primer_bind     complement(3742..3758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3766..3782)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3790..3820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3835..3856)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4144..4732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4906..5763)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5764..5868)
                     /label=AmpR promoter
     misc_feature    5900..6491
                     /label=Candida glabrata CEN/ARS
                     /note="Candida glabrata CEN/ARS"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      241 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt
      301 tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagagacc
      361 gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc
      421 ttgaaaattt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa
      481 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
      541 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagttttg gatctaaaat
      601 gggcaccact cttgacgaca cggcttaccg ataccgcacc agtgtcccgg gggacgccga
      661 ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac
      721 cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc
      781 cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgaccctg actcccggac
      841 gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg
      901 ctggaaccgc cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg
      961 ggtcgggcgc gcgttgatgg ggctcgcgac ggagttcgcc cgcgagctgg gcgccgggca
     1021 cctctggctg gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg
     1081 gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca
     1141 ggcgctctac atgagcatgc cctgccccta agtcgagtca tgtaattagt tatgtcacgc
     1201 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     1261 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     1321 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     1381 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg tatgcggtgt
     1441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     1501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     1561 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     1621 cgttgtaaaa cgacggccag tgaattcgag ctctgttatt gattatttat ttatttgttg
     1681 ttattttatt gtattttttt ttaaaaagct actaatacac aacaaaatgt aaaccacaaa
     1741 ctctcccaaa cagaacgaac accctggctt tatatacaaa atttcttatc ccgaaatgat
     1801 tgtcaacaag ataaacaaaa agaagccacc acaggaaccc taccccccct ctctcccacc
     1861 cccatcgtgt cttcccttcc accttccacc cttcacctgg ccatagtaac ttgaggtgtg
     1921 cacactccag aacggtcgac ccactacgga tctgaattcc agtgcgaagt tgcgggaacg
     1981 gatttattat gaccaggcgg ctactaagaa gctcaaacca tcaaaaacgc gcgaagaaac
     2041 tggtctaccc gtagcaagtt actattctcc aaattagtgt ttgtcgtgtg ccgtgcgtcg
     2101 tgggtagacc gagattttcg cgcgcctcag gtggttcgag ggcaaataac catagttaag
     2161 ccgttcccat tctttctcac atgtacgtgc cggacgcttc gatgaaattt ttgtgaatcg
     2221 tttcgttgct tctgtgacca caaaacagcc tggagaggag gcgttgcgta aggggggggg
     2281 gcctgatgag aaacagatga cacaacgcag aactggctgc agtgcaaagg catttcctct
     2341 gccacacaca ctaccataac cagtctcggt attaactggc tcaacaaacg ataagagcca
     2401 aagaggaaga aaaaaaggta tttaaaggat gtatttcttt atctcctgat tagatttctt
     2461 acttgtacag aaactgtaac taaaaaaaac aacaacaaaa cacaacacat acatatctag
     2521 aactagtgga tcccccgggc tgcaggaatt catgtctaaa ggtgaagaat tattcactgg
     2581 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     2641 cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac
     2701 tggtaaattg ccggttccat ggccaacctt agtcactact ttcggttatg gtgttcaatg
     2761 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     2821 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     2881 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     2941 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     3001 ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa
     3061 cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga
     3121 tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga
     3181 tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac
     3241 ccatggtatg gatgaattgt acaaataact gcaggtcgac ctcgagaaca caacccacag
     3301 ctaccaccat caacaatatt tatatatata acgtacacat agaaatcaca caaacagagt
     3361 atttattctt aactacatga actaccatca gaccgtctgg gccactatat aatgtgccat
     3421 tcataaacgt gatcacttta cgtagcaggc aaccccaggt gaaaattttt cagcgagctg
     3481 ccagattgtc aggtgaaaaa ctgaaaaaaa cttctgggcg atgagcttgt ggcgggaaat
     3541 taagtatata aagcagttag tttcttctgc ttcttgtggt tctgggtatt cttgtaatat
     3601 ttggaagaat agaaaatcaa gactactgcc tttcttttca tattatagag gaagaaatac
     3661 gcacgaacac gatatagagg taaaggtacc cggggatcct ctagagtcga cctgcaggca
     3721 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3781 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3841 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3901 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4021 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4141 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4201 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4261 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4321 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4381 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4441 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4501 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4561 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4621 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4681 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4741 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4801 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4861 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4921 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4981 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5041 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5101 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5161 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5221 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5281 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5341 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5401 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5461 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5521 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5581 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5641 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5701 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5761 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5821 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5881 aaaagtgcca cctgacgtcg ggatctctaa tgatgcaatt tttgttttgt acactaagat
     5941 tctatttcgg aactcttacc catgtcatta tttactttat tttcattatt gtaatttttt
     6001 aagcttttat tatgaattat ttaaatacaa ttctttttga tcatgtgaca gacatcactg
     6061 aaagatgtgc tctgcgttat tagtcagatt gaaatataca ttttcatgta gatatgatgt
     6121 tcttacttta aatgttgttc catttgctag cttaaataga cgatattata aatttaagta
     6181 ttcactaaaa atctgaccca ttaatgttag aattagtatt attttttgtg atattacatt
     6241 tttatatttt ggatttcaac caatgctctg cgatatgatt caacagaata atatgtacaa
     6301 gctactttaa ttcatatata ctaatagatt cacagatcca gtacgactgc aagaatttac
     6361 gttttgtttg aaatggtagt agctacacta aattaacaat gatcgattaa tccacatcca
     6421 cagtgtgtaa tcatcctaat gaaaacaaga ataactacaa aaatgaatat aaactttttg
     6481 catgttagtc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6541 gtatcacgag gccctttcgt c
//
