LOCUS V030764 6212 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030764 VERSION V030764 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 4..30 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 55..2199 /label="ZDHHC5(NM_015457)" /note="ZDHHC5(NM_015457)" /gene="ZDHHC5" CDS 2257..2508 /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" /label="VC155" polyA_signal 2523..2657 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(2768..2784) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2792..2808 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2816..2846) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2861..2882 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3170..3758) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3929..4789) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(4790..4894) /gene="bla" /label="AmpR promoter" primer_bind 5368..5384 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 5411..5714 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 5715..5918 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 6056..6152 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" ORIGIN 1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattatgccc 61 gcagagtctg gaaagagatt caaacccagc aagtatgtcc cggtctctgc agccgccatc 121 ttcctagtgg gagctacgac actcttcttt gcctttacgt gtccaggact aagcctgtat 181 gtgtcacctg cagtgcccat ctacaatgca attatgtttc tctttgtgtt ggccaacttc 241 agcatggcca ccttcatgga cccagggatt ttccctcgag ctgaggagga tgaggacaag 301 gaagatgatt tccgagctcc cctttacaaa acagtggaga taaagggcat ccaggtgcgc 361 atgaaatggt gtgccacctg ccgcttttac cgtccccctc gatgttccca ctgcagtgtc 421 tgtgacaact gtgtggagga atttgatcat cactgcccct gggtgaataa ctgtattggt 481 cgccggaact accgttattt tttccttttc ctcctttccc tgacagccca cattatgggt 541 gtgtttggct ttggcctcct ttatgtcctc taccacatag aggaactctc aggggtccgc 601 acggctgtca caatggcagt aatgtgtgtg gctggcttat tcttcatccc tgtagctggc 661 ctcacgggat ttcacgtggt tctggtggcc aggggacgca caaccaatga acaggttacg 721 ggtaaattcc ggggaggtgt gaaccccttc accaatggct gctgtaacaa tgtcagccgt 781 gttctctgca gttctccagc acccaggtat ttggggagac caaagaaaga gaagacaatt 841 gtaatcagac ctcccttcct tcgaccagaa gtttcagatg ggcagataac tgtgaagatc 901 atggataatg gcatccaggg agagctgagg agaacaaagt ctaagggaag cctggagata 961 acagagagcc agtctgcaga tgctgaacct ccacctcctc ctaagccaga cctgagccgt 1021 tacacagggt tgcgaacaca cctcggcttg gctactaatg aggatagtag cttattggcc 1081 aaggacagcc ccccgacacc taccatgtac aagtatcggc cgggttacag tagcagcagt 1141 acgtcagctg ccatgccgca ttcctccagc gccaagttga gtcgtgggga cagcttgaag 1201 gagccaacct caattgcaga gagcagccgt caccccagct accgctcaga gcccagcttg 1261 gaaccagaga gcttccgttc tcctaccttt ggcaaaagtt ttcacttcga tccactatcc 1321 agtggctcac gctcctccag cctcaagtca gcccagggca caggctttga gctgggccag 1381 ttgcaatcca ttcgttcaga gggcaccacc tccacctcct ataagagcct ggccaaccag 1441 acacgcaatg gaagcctatc ttatgacagc ttgctcacac cttcagacag ccctgatttt 1501 gagtcagtgc aggcagggcc tgagccagac ccacctttag gctatacctc tcccttcctg 1561 tcagccaggc tggcccagca acgggaagct gagaggcacc cacgtttggt gccaactggc 1621 ccaacacacc gagagccctc accagtccgt tacgacaatc tgtcgcgcca cattgtggcc 1681 tctctccagg aacgagagaa gttgctgcgc cagtcacccc cactcccggg ccgtgaggaa 1741 gaaccaggct tgggggactc aggcattcag tcaacaccag gctcgggcca tgcccctcgt 1801 actagttcct cctcagatga ttcaaagaga tcacctttgg gcaagactcc actgggacgc 1861 ccagctgtcc cccgttttgg caagccagat gggctaaggg gccggggagt agggtcccct 1921 gaaccaggcc caacagcccc atacctgggc cgatcgatgt cttacagcag ccaaaaagcc 1981 caacctggtg tctctgagac agaagaagtg gccttgcagc cattactgac acccaaagat 2041 gaagtacagc tgaagaccac ctacagcaaa tccaacgggc agcccaagag cttaggctca 2101 gcctcccctg gcccaggcca gccacctctc agtagcccca cgaggggagg agtcaagaag 2161 gtgtcagggg ttggtggtac cacctatgag atttcggtgg gtacccgtcc ggcgtgcaaa 2221 atcccgaacg acctgaaaca gaaagtcatg aaccacgaca agcagaagaa cggcatcaag 2281 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac 2341 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 2401 taccagtcca aactgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 2461 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg 2521 gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 2581 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 2641 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 2701 aggtgtggga ggttttttcg gatcctctag agtcgatctg caggcatgct agcttggcgt 2761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 2821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 2881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 2941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 3001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3601 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 3961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 4261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 4321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 4381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 4441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 4561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 4621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 4681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 4741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 4861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 4921 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 4981 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 5041 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 5101 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 5161 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 5221 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 5281 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 5341 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagttc gagcttgcat 5401 gcctgcaggt cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 5461 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 5521 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 5581 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 5641 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 5701 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 5761 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 5821 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 5881 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg 5941 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 6001 tccggactct agaggatccg gtactagagg aactgaaaaa ccagaaagtt aactggtaag 6061 tttagtcttt ttgtctttta tttcaggtcc cggatccggt ggtggtgcaa atcaaagaac 6121 tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct 6181 aaaagctgcg gaattgtacc cgcgggccca cc //