LOCUS V035057 4782 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035057 VERSION V035057 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter complement(join(4750..4782,1..72)) /gene="bla" /label="AmpR promoter" primer_bind 546..562 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 589..892 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 893..1096 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 1234..1330 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" CDS 1394..1420 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1448..2458 /label="ZDHHC15(NM_144969)" /note="ZDHHC15(NM_144969)" /gene="ZDHHC15" polyA_signal 2484..2618 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(2728..2744) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2752..2768 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2776..2806) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2821..2842 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3130..3718) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3889..4749) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" ORIGIN 1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact 601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg 1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt 1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt 1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct 1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg 1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg 1441 aattcccatg cggcgaggct ggaagatggc tctgtctggg gggctgcggt gctgccgccg 1501 ggtactgtcc tgggtgccag tgctcgttat tgtcctcgtc gtgctctggt cctactatgc 1561 ctacgtcttt gaactctgcc tggtgactgt tttgagccca gcagaaaaag ttatttacct 1621 catactctac catgccatct ttgtgttctt tacctggacc tactggaagt ctatctttac 1681 actcccacag cagccaaacc agaagttcca cttgtcctac acagacaagg agcgctatga 1741 aaatgaagaa agacctgagg tccagaagca gatgcttgtt gatatggcca aaaagctacc 1801 ggtttacaca agaactggaa gtggagctgt acgattctgt gaccggtgtc atctgatcaa 1861 gccagaccgc tgccaccact gctctgtctg tgctatgtgt gtgttaaaaa tggatcatca 1921 ctgcccttgg gttaataact gcattggatt ttccaactac aaattcttcc ttcaattctt 1981 agcttactct gttctctact gcctgtacat tgctacgaca gtcttcagct atttcatcaa 2041 atactggaga ggggaattac ccagtgttcg ctctaagttc catgtccttt ttcttctctt 2101 tgtggcctgc atgttttttg tcagccttgt gattctcttt ggttaccatt gttggcttgt 2161 cagcagaaac aaaaccacct tagaggcctt ctgcactcca gtgtttacaa gtggcccaga 2221 gaaaaatggg ttcaaccttg gcttcatcaa gaatatccag caggtgtttg gagataagaa 2281 gaagttctgg ttaataccta ttggttccag ccctggtgat ggacactcct tccctatgag 2341 gtctatgaat gagtcacaga acccactgct agcaaatgaa gaaacctggg aagacaacga 2401 ggatgacaac caagattatc cagaaggctc atcatctctt gctgtggaaa cggaaacata 2461 gctcgaggta ccgcggccgc ggggatccag acatgataag atacattgat gagtttggac 2521 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 2581 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 2641 ttatgtttca ggttcagggg gaggtgtggg aggtttttcg gatcctctag agtcgatctg 2701 caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 2761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 2821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 2881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 2941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3541 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 3601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 3721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 3781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 3841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 3901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 3961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 4621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 4681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 4741 aatactcata ctcttccttt ttcaatatta ttgaagcatt ta //