LOCUS V028138 6507 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028138 VERSION V028138 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 905..1558 /codon_start=1 /gene="" /product="glutathione S-transferase from Schistosoma japonicum" /transl_table=1 /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" /label="GST" CDS 1589..2335 /label="MITD1(NM_138798)" /note="MITD1(NM_138798)" /gene="MITD1" polyA_signal 2371..2595 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2641..3069 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3083..3412 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3424..4023 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 4183..4304 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4353..4369) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4377..4393 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4401..4431) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4446..4467 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4755..5340) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5511..6371) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6372..6476) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 caccatgtcc cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact 961 tcttttggaa tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga 1021 taaatggcga aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat 1081 tgatggtgat gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca 1141 caacatgttg ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt 1201 tttggatatt agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa 1261 agttgatttt cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca 1321 taaaacatat ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct 1381 tgatgttgtt ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt 1441 taaaaaacgt attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat 1501 agcatggcct ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaagg 1561 tggaggcgga tcaggaggcg gtggatctat ggcgaagtcc gggctgaggc aggacccgca 1621 gagcacagct gcagccactg tgctaaagcg ggcagtagaa ctagattcgg agtcgcggta 1681 tccgcaggct ctggtgtgtt accaagaggg gattgatctg ctcctgcagg ttctgaaagg 1741 taccaaagat aatactaaga gatgtaatct cagagaaaaa atttccaaat acatggacag 1801 agcggaaaac ataaagaagt acttggacca agaaaaagaa gatggaaaat atcacaagca 1861 aattaaaata gaagagaatg caacaggttt cagttatgag tcactttttc gcgaatacct 1921 taatgagaca gttacagaag tttggataga agatccttat attagacata ctcatcagct 1981 gtataacttt cttcgatttt gtgagatgct tattaagaga ccatgtaaag taaaaactat 2041 tcaccttctc acctctctgg atgaaggcat tgagcaagtg cagcaaagta gaggcctgca 2101 agaaatagaa gagtcactca ggagtcacgg agtgctgttg gaagttcaat actcttcttc 2161 aatacatgac cgagaaatta ggttcaacaa tggatggatg attaagattg gaaggggact 2221 tgattatttt aagaaaccac agagtcgttt ttcccttgga tattgtgatt ttgatttaag 2281 accatgtcat gaaacaacag tagacatttt tcataagaag catacaaaaa atatatgaac 2341 cggtaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 2401 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 2461 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 2521 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 2581 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 2641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 2701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 2761 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 2821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 2881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 2941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 3001 aagggatttt ggccatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 3061 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3181 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3241 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3301 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 3361 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 3421 gggatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 3481 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 3541 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 3601 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 3661 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 3721 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 3781 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 3841 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 3901 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 3961 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 4021 tgattcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 4081 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 4141 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 4201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 4261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 4321 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 4381 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 4441 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 4501 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4561 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4621 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4681 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4741 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4801 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4861 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4921 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 4981 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 5041 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 5101 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 5161 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 5221 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 5281 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 5341 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 5401 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 5461 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 5521 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 5581 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 5641 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 5701 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 5761 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 5821 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 5881 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 5941 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 6001 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 6061 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 6121 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 6181 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 6241 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 6301 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 6361 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 6421 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 6481 tttccccgaa aagtgccacc tgacgtc //