LOCUS V034374 6445 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034374 VERSION V034374 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 937..2196 /label="DCP2(NM_152624)" /note="DCP2(NM_152624)" /gene="DCP2" CDS 2209..2274 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2309..2533 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2579..3007 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3021..3350 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3362..3961 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 4121..4242 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4291..4307) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4315..4331 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4339..4369) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4384..4405 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4693..5278) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5449..6309) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6310..6414) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcga tatcggatcc gccaccatgg agaccaaacg ggtggagatt 961 cccggcagcg tcctggacga tctctgcagc cgatttattt tgcatattcc cagcgaggaa 1021 agagacaatg caatccgagt gtgttttcag attgaacttg cccattggtt ttacttggat 1081 ttctacatgc agaacacacc aggattacct cagtgtggga taagagactt tgctaaagct 1141 gtcttcagtc attgtccgtt tttgctgcct caaggtgaag atgtggaaaa agttttggat 1201 gaatggaagg aatataaaat gggagtacca acatatggtg caattattct tgatgagaca 1261 cttgaaaatg tactactagt tcaggggtac ctagcaaaat caggctgggg atttccaaaa 1321 ggaaaagtaa ataaagaaga agctcctcat gattgtgctg ctagagaggt ctttgaagaa 1381 actggttttg atatcaaaga ctatatttgt aaggatgatt acattgaact tcgaatcaat 1441 gaccagcttg ctcgtttgta catcattcca ggaattccaa aagacacaaa atttaaccca 1501 aaaactagaa gagaaattcg gaacattgag tggttctcta ttgagaaatt gccttgtcat 1561 agaaatgata tgacccccaa atccaaactt ggtttggcac ctaacaaatt ttttatggcc 1621 attcccttta tcagaccatt aagggactgg ctttctcgaa gatttggcga ttcctcagac 1681 agtgacaatg gattttcctc aactggtagc acgccggcta aacccactgt ggaaaaattg 1741 agtcgaacca aattccgcca cagtcagcag ttatttcctg acggttctcc tggtgaccag 1801 tgggtaaagc acaggcaacc actgcagcaa aagccatata ataatcattc tgaaatgtct 1861 gaccttttaa aaggaaagaa tcaaagtatg aggggaaatg gcagaaaaca gtatcaagat 1921 tcacctaatc aaaagaaaag aacaaatggg cttcagccag caaagcagca gaattctttg 1981 atgaagtgtg aaaagaaact tcatccacgg aaacttcagg ataattttga aacagatgct 2041 gtatatgact tgcctagctc cagtgaagac cagttgctag aacatgctga gggacagccc 2101 gtggcatgta atggacattg caagttcccc ttttcatcca gagccttttt gagtttcaag 2161 tttgaccata atgctataat gaaaatcttg gaccttctcg agaccggtga ctacaaagac 2221 catgacggtg attataaaga tcatgacatc gattacaagg atgacgatga caagtgaccg 2281 gtaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 2341 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 2401 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 2461 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 2521 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2581 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2641 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2701 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 2761 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2821 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 2881 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 2941 gggattttgg ccatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3001 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3061 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3121 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3181 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3241 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 3301 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 3361 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 3421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 3481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 3541 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 3601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 3661 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 3721 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 3781 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 3841 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 3901 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 3961 attcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 4021 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 4081 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 4141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 4201 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 4261 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 4321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 4381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 4621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 4921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 5161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5221 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 6001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 6061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 6121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6421 tccccgaaaa gtgccacctg acgtc //