LOCUS V026545 7126 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026545 VERSION V026545 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..3034 /label="ITGB1(NM_002211)" /note="ITGB1(NM_002211)" /gene="ITGB1" CDS 3083..3793 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 3914..4035 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4042..4497) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4524..4628 /gene="bla" /label="AmpR promoter" promoter 4630..4987 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5022..5816 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6048..6095 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6424..7012 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atgaatttac aaccaatttt 661 ctggattgga ctgatcagtt cagtttgctg tgtgtttgct caaacagatg aaaatagatg 721 tttaaaagca aatgccaaat catgtggaga atgtatacaa gcagggccaa attgtgggtg 781 gtgcacaaat tcaacatttt tacaggaagg aatgcctact tctgcacgat gtgatgattt 841 agaagcctta aaaaagaagg gttgccctcc agatgacata gaaaatccca gaggctccaa 901 agatataaag aaaaataaaa atgtaaccaa ccgtagcaaa ggaacagcag agaagctcaa 961 gccagaggat attactcaga tccaaccaca gcagttggtt ttgcgattaa gatcagggga 1021 gccacagaca tttacattaa aattcaagag agctgaagac tatcccattg acctctacta 1081 ccttatggac ctgtcttact caatgaaaga cgatttggag aatgtaaaaa gtcttggaac 1141 agatctgatg aatgaaatga ggaggattac ttcggacttc agaattggat ttggctcatt 1201 tgtggaaaag actgtgatgc cttacattag cacaacacca gctaagctca ggaacccttg 1261 cacaagtgaa cagaactgca ccagcccatt tagctacaaa aatgtgctca gtcttactaa 1321 taaaggagaa gtatttaatg aacttgttgg aaaacagcgc atatctggaa atttggattc 1381 tccagaaggt ggtttcgatg ccatcatgca agttgcagtt tgtggatcac tgattggctg 1441 gaggaatgtt acacggctgc tggtgttttc cacagatgcc gggtttcact ttgctggaga 1501 tgggaaactt ggtggcattg ttttaccaaa tgatggacaa tgtcacctgg aaaataatat 1561 gtacacaatg agccattatt atgattatcc ttctattgct caccttgtcc agaaactgag 1621 tgaaaataat attcagacaa tttttgcagt tactgaagaa tttcagcctg tttacaagga 1681 gctgaaaaac ttgatcccta agtcagcagt aggaacatta tctgcaaatt ctagcaatgt 1741 aattcagttg atcattgatg catacaattc cctttcctca gaagtcattt tggaaaacgg 1801 caaattgtca gaaggcgtaa caataagtta caaatcttac tgcaagaacg gggtgaatgg 1861 aacaggggaa aatggaagaa aatgttccaa tatttccatt ggagatgagg ttcaatttga 1921 aattagcata acttcaaata agtgtccaaa aaaggattct gacagcttta aaattaggcc 1981 tctgggcttt acggaggaag tagaggttat tcttcagtac atctgtgaat gtgaatgcca 2041 aagcgaaggc atccctgaaa gtcccaagtg tcatgaagga aatgggacat ttgagtgtgg 2101 cgcgtgcagg tgcaatgaag ggcgtgttgg tagacattgt gaatgcagca cagatgaagt 2161 taacagtgaa gacatggatg cttactgcag gaaagaaaac agttcagaaa tctgcagtaa 2221 caatggagag tgcgtctgcg gacagtgtgt ttgtaggaag agggataata caaatgaaat 2281 ttattctggc aaattctgcg agtgtgataa tttcaactgt gatagatcca atggcttaat 2341 ttgtggagga aatggtgttt gcaagtgtcg tgtgtgtgag tgcaacccca actacactgg 2401 cagtgcatgt gactgttctt tggatactag tacttgtgaa gccagcaacg gacagatctg 2461 caatggccgg ggcatctgcg agtgtggtgt ctgtaagtgt acagatccga agtttcaagg 2521 gcaaacgtgt gagatgtgtc agacctgcct tggtgtctgt gctgagcata aagaatgtgt 2581 tcagtgcaga gccttcaata aaggagaaaa gaaagacaca tgcacacagg aatgttccta 2641 ttttaacatt accaaggtag aaagtcggga caaattaccc cagccggtcc aacctgatcc 2701 tgtgtcccat tgtaaggaga aggatgttga cgactgttgg ttctatttta cgtattcagt 2761 gaatgggaac aacgaggtca tggttcatgt tgtggagaat ccagagtgtc ccactggtcc 2821 agacatcatt ccaattgtag ctggtgtggt tgctggaatt gttcttattg gccttgcatt 2881 actgctgata tggaagcttt taatgataat tcatgacaga agggagtttg ctaaatttga 2941 aaaggagaaa atgaatgcca aatgggacac gggtgaaaat cctatttata agagtgccgt 3001 aacaactgtg gtcaatccga agtatgaggg aaaaggtgga ggcggatcag gaggcggtgg 3061 atcggatcca ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat 3121 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 3181 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 3241 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 3301 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 3361 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 3421 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 3481 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 3541 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 3601 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 3661 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 3721 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 3781 gctgtacaag tagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 3841 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 3901 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 3961 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 4021 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 4081 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 4141 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 4201 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 4261 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 4321 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 4381 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 4441 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 4501 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 4561 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 4621 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 4681 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 4741 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 4801 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 4861 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 4921 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 4981 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 5041 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 5101 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 5161 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 5221 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 5281 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 5341 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 5401 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 5461 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 5521 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 5581 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 5641 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 5701 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 5761 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 5821 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 5881 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 5941 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 6001 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 6061 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 6121 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 6181 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 6241 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 6301 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 6361 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 6421 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 6481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 6541 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 6601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 6661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 6721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 6781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 6841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 6901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 6961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 7021 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 7081 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat //