LOCUS V038646 6396 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038646 VERSION V038646 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 641..2293 /label="CPSF6(NM_007007)" /note="CPSF6(NM_007007)" /gene="CPSF6" CDS 2342..3061 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3184..3305 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3312..3767) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3794..3898 /gene="bla" /label="AmpR promoter" promoter 3900..4257 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4292..5086 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5318..5365 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5694..6282 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcgccacc atggcggacg gcgtggacca 661 catagacatt tacgcggatg tcggcgaaga gttcaaccag gaagctgaat atggtgggca 721 tgatcagata gatttgtatg acgatgtcat atctccatct gcaaataatg gagatgcccc 781 agaagaccga gattacatgg atactctccc accaactgtt ggtgatgatg tgggtaaagg 841 agcagcacca aatgttgtct atacatatac tggaaagaga attgcattat atattggaaa 901 tctaacatgg tggacaacag atgaagactt aactgaagca gttcattctt tgggagtaaa 961 tgatattttg gagataaaat tttttgaaaa tcgggcaaat ggccagtcaa aggggtttgc 1021 ccttgttggt gttggatctg aagcatcttc aaaaaagtta atggatctgt tacctaaaag 1081 agaacttcat ggtcagaatc ctgttgtaac tccatgcaat aaacagttcc tgagtcaatt 1141 tgaaatgcag tccaggaaaa ctacacaatc aggacaaatg tctggggaag gtaaagctgg 1201 tcctccagga ggcagttccc gtgcagcatt tccacaaggt ggtagaggac ggggccgttt 1261 tccaggggct gttcctggtg gggacagatt tcctgggcca gcaggaccag gagggccacc 1321 cccacctttt ccagctggac agactccacc acgtccaccc ttaggtcctc caggcccacc 1381 tggtccacca ggtcctccac ctcctggtca ggttctgcct cctcctctag ctgggcctcc 1441 taatcgagga gatcgccctc caccaccagt tctttttcct ggacaacctt ttgggcagcc 1501 tccattgggt ccacttcctc ctggccctcc acctccagtt ccaggctacg gcccccctcc 1561 tggcccacca cctccacaac agggaccacc tccacctcca ggcccctttc cacctcgtcc 1621 acccggtcca cttgggccac cccttacact agctcctcct ccgcatcttc ctggaccacc 1681 tccaggtgcc ccaccgccag ctccgcatgt gaacccagct ttctttcctc caccaactaa 1741 cagtggcatg cctacatcag atagccgagg tccaccacca acagatccat atgggcgacc 1801 tccaccatat gataggggtg actatggccc ccctggaagg gaaatggata ctgcaagaac 1861 gccattgagt gaagctgaat ttgaagaaat catgaataga aatagggcaa tctcaagcag 1921 tgctatttcg agagctgtgt ctgatgccag tgctggtgat tatgggagtg ctattgagac 1981 actggtaact gcaatttctt taattaaaca atccaaagta tctgctgatg atcgttgcaa 2041 agttcttatt agttctttgc aagattgcct tcatggaatt gagtccaagt cttatggttc 2101 tggatcaaga cgtgaacgat caagagagag ggaccatagt agatcacgag aaaagagtcg 2161 acgtcataaa tcccgtagta gagaccgtca tgacgattat tacagagaga gaagcagaga 2221 acgagagagg caccgggatc gtgaccgaga ccgtgaccga gagcgtgacc gagagcgcga 2281 atatcgtcat cgtggtggag gcggatcagg aggcggtgga tcggatccac cggtcgccac 2341 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 2401 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 2461 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 2521 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 2581 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2641 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2701 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 2761 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 2821 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 2881 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 2941 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 3001 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta 3061 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 3121 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 3181 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3301 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 3361 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 3421 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 3481 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 3541 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 3601 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 3661 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 3721 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 3781 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 3841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 3901 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 4021 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4081 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 4141 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4201 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 4261 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 4441 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 4801 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 5101 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 5161 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 5221 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 5281 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 5341 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 5401 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 5461 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 5521 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 5581 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5641 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5761 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5821 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 5881 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5941 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 6001 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 6061 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 6121 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 6181 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 6241 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 6301 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 6361 cctgattctg tggataaccg tattaccgcc atgcat //