LOCUS V027309 5486 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027309 VERSION V027309 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 141..157 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 318..697 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 698..901 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 931..996 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 1000..1797 /label="REC114(NM_001042367)" /note="REC114(NM_001042367)" /gene="REC114" polyA_signal 1827..2449 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" promoter 2478..2807 /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(2846..2864) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(2878..2894) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2902..2918 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2926..2956) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2971..2992 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3280..3868) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4039..4899) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(4900..5004) /gene="bla" /label="AmpR promoter" rep_origin 5031..5459 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa 961 gatcatgaca tcgattacaa ggatgacgat gacaagctta tggcggaggc aggaaaagtg 1021 cccttgagcc tcgggcttac cggaggagaa gcggcagagt ggcctctgca gcggtacgcc 1081 cgctgcatac cctcaaacac cagagaccca cctgggccat gcctggaagc tgggacagcc 1141 ccctgcccca catggaaggt ttttgattcc aatgaagaat ctggatatct tgttctcacc 1201 atagttatat caggtcattt cttcattttc caaggacaga cactactgga agggttttca 1261 ctcattggta gcaaggactg gttgaagatt gtaagacgcg tggattgtct gttgtttgga 1321 acaacgataa aggacaagag tcgcctgttt cgagtacagt tcagtggaga gtcaaaggag 1381 caggcgctgg aacactgctg cagttgtgtt cagaagctgg cacaatacat aaccgtgcag 1441 gtgcctgatg gaaacatcca ggagcttcag ctgattcctg gcccacccag ggcaactgaa 1501 agtcaaggga aggattctgc aaagagtgtc ccacggcagc ctggatccca ccagcactca 1561 gaacaacagc aagtgtgtgt aacagcgggc acaggcgctc cagacggaag gacctcactg 1621 acgcagttag ctcagactct tctggcatcg gaggagctgc cccatgtcta tgaacaatct 1681 gcatggggtg cagaagagtt aggccccttc ctacgtttgt gccttatgga tcagaatttc 1741 ccagcatttg tggaagaggt agaaaaggaa ctgaaaaagc tggcgggttt gagaaattaa 1801 ggtaccagtc gactctagag gatcccgggt ggcatccctg tgacccctcc ccagtgcctc 1861 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt 1921 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat 1981 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa 2041 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga 2101 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct 2161 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact 2221 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa 2281 ccactgctcc cttccctgtc cttctgattt taaaataact ataccagcag gaggacgtcc 2341 agacacagca taggctacct ggccatgccc aaccggtggg acatttgagt tgcttgcttg 2401 gcactgtcct ctcatgcgtt gggtccactc agtagatgcc tgttgaattg ggtacgcggc 2461 cagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 2521 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 2581 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 2641 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 2701 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 2761 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctcgagg 2821 aactgaaaaa ccagaaagtt aattccctat agtgagtcgt attaaattcg taatcatgtc 2881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 2941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 3001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 3061 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 3121 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3181 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3241 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3301 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3361 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3421 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3481 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3541 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3601 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3661 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3721 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3781 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3841 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3901 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3961 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 4021 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4081 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 4141 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 4201 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 4261 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 4321 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 4381 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4441 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4501 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4561 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4621 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 4681 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 4741 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 4801 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 4861 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 4921 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 4981 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg 5041 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 5101 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 5161 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 5221 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 5281 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 5341 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 5401 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 5461 taacaaaata ttaacgctta caattt //