LOCUS V042684 6410 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042684 VERSION V042684 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 141..157 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 318..697 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 698..901 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 935..958 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 962..2200 /label="ADRB2(NM_000024)" /note="ADRB2(NM_000024)" /gene="ADRB2" CDS 2222..2740 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNG IKANFKIRHNIE" /label="VN173" polyA_signal 2751..3373 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" promoter 3402..3731 /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(3770..3788) /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" primer_bind complement(3802..3818) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3826..3842 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3850..3880) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3895..3916 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4204..4792) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4963..5823) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5824..5928) /gene="bla" /label="AmpR promoter" rep_origin 5955..6383 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct 961 tatggggcaa cccgggaacg gcagcgcctt cttgctggca cccaatggaa gccatgcgcc 1021 ggaccacgac gtcacgcagg aaagggacga ggtgtgggtg gtgggcatgg gcatcgtcat 1081 gtctctcatc gtcctggcca tcgtgtttgg caatgtgctg gtcatcacag ccattgccaa 1141 gttcgagcgt ctgcagacgg tcaccaacta cttcatcact tcactggcct gtgctgatct 1201 ggtcatgggc ctggcagtgg tgccctttgg ggccgcccat attcttatga aaatgtggac 1261 ttttggcaac ttctggtgcg agttttggac ttccattgat gtgctgtgcg tcacggccag 1321 cattgagacc ctgtgcgtga tcgcagtgga tcgctacttt gccattactt cacctttcaa 1381 gtaccagagc ctgctgacca agaataaggc ccgggtgatc attctgatgg tgtggattgt 1441 gtcaggcctt acctccttct tgcccattca gatgcactgg taccgggcca cccaccagga 1501 agccatcaac tgctatgcca atgagacctg ctgtgacttc ttcacgaacc aagcctatgc 1561 cattgcctct tccatcgtgt ccttctacgt tcccctggtg atcatggtct tcgtctactc 1621 cagggtcttt caggaggcca aaaggcagct ccagaagatt gacaaatctg agggccgctt 1681 ccatgtccag aaccttagcc aggtggagca ggatgggcgg acggggcatg gactccgcag 1741 atcttccaag ttctgcttga aggagcacaa agccctcaag acgttaggca tcatcatggg 1801 cactttcacc ctctgctggc tgcccttctt catcgttaac attgtgcatg tgatccagga 1861 taacctcatc cgtaaggaag tttacatcct cctaaattgg ataggctatg tcaattctgg 1921 tttcaatccc cttatctact gccggagccc agatttcagg attgccttcc aggagcttct 1981 gtgcctgcgc aggtcttctt tgaaggccta tgggaatggc tactccagca acggcaacac 2041 aggggagcag agtggatatc acgtggaaca ggagaaagaa aataaactgc tgtgtgaaga 2101 cctcccaggc acggaagact ttgtgggcca tcaaggtact gtgcctagcg ataacattga 2161 ttcacaaggg aggaattgta gtacaaatga ctcactgctg tctagaagat ccatcgccac 2221 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 2281 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 2341 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac 2401 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa 2461 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 2521 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 2581 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 2641 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa 2701 cggcatcaag gccaacttca agatccgcca caacatcgag tagggatccc gggtggcatc 2761 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 2821 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata 2881 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 2941 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 3001 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc 3061 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca 3121 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 3181 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat 3241 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg 3301 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga 3361 tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag ttagggtgtg 3421 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3481 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3541 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 3601 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3661 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3721 gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc ctatagtgag 3781 tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 3841 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 3901 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 3961 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 4141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 4201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 4261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 4321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 4381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 4441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 4501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 4561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 4621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 4681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 4741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 4801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 4861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 4921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 4981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 5101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 5161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 5221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 5281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 5341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 5401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 5461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 5521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 5581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 5641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 5701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 5761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 5821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 5881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 5941 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 6001 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 6061 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 6121 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 6181 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 6241 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 6301 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 6361 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt //