LOCUS V020762 5345 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020762 VERSION V020762 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 4..30 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 58..1332 /label="SMAD3(NM_005902)" /note="SMAD3(NM_005902)" /gene="SMAD3" CDS 1390..1641 /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /transl_table=1 /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" /label="VC155" polyA_signal 1656..1790 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(1901..1917) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 1925..1941 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(1949..1979) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 1994..2015 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(2303..2891) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3062..3922) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(3923..4027) /gene="bla" /label="AmpR promoter" primer_bind 4501..4517 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 4544..4847 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 4848..5051 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 5189..5285 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" ORIGIN 1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcccatg 61 tcgtccatcc tgcctttcac tcccccgatc gtgaagcgcc tgctgggctg gaagaagggc 121 gagcagaacg ggcaggagga gaaatggtgc gagaaggcgg tcaagagcct ggtcaagaaa 181 ctcaagaaga cggggcagct ggacgagctg gagaaggcca tcaccacgca gaacgtcaac 241 accaagtgca tcaccatccc caggtccctg gatggccggt tgcaggtgtc ccatcggaag 301 gggctccctc atgtcatcta ctgccgcctg tggcgatggc cagacctgca cagccaccac 361 gagctacggg ccatggagct gtgtgagttc gccttcaata tgaagaagga cgaggtctgc 421 gtgaatccct accactacca gagagtagag acaccagttc tacctcctgt gttggtgcca 481 cgccacacag agatcccggc cgagttcccc ccactggacg actacagcca ttccatcccc 541 gaaaacacta acttccccgc aggcatcgag ccccagagca atattccaga gaccccaccc 601 cctggctacc tgagtgaaga tggagaaacc agtgaccacc agatgaacca cagcatggac 661 gcaggttctc caaacctatc cccgaatccg atgtccccag cacataataa cttggacctg 721 cagccagtta cctactgcga gccggccttc tggtgctcca tctcctacta cgagctgaac 781 cagcgcgtcg gggagacatt ccacgcctcg cagccatcca tgactgtgga tggcttcacc 841 gacccctcca attcggagcg cttctgccta gggctgctct ccaatgtcaa caggaatgca 901 gcagtggagc tgacacggag acacatcgga agaggcgtgc ggctctacta catcggaggg 961 gaggtcttcg cagagtgcct cagtgacagc gctatttttg tccagtctcc caactgtaac 1021 cagcgctatg gctggcaccc ggccaccgtc tgcaagatcc caccaggatg caacctgaag 1081 atcttcaaca accaggagtt cgctgccctc ctggcccagt cggtcaacca gggctttgag 1141 gctgtctacc agttgacccg aatgtgcacc atccgcatga gcttcgtcaa aggctgggga 1201 gcggagtaca ggagacagac tgtgaccagt accccctgct ggattgagct gcacctgaat 1261 gggcctttgc agtggcttga caaggtcctc acccagatgg gctccccaag catccgctgt 1321 tccagtgtgt ctggtacccg tccggcgtgc aaaatcccga acgacctgaa acagaaagtc 1381 atgaaccacg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc 1441 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 1501 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc 1561 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 1621 ggcatggacg agctgtacaa gtaagcggcc gcggggatcc agacatgata agatacattg 1681 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 1741 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca 1801 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc 1861 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt 1921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 1981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 2041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 2101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 2701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 3361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 3661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 4141 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 4201 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 4261 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 4321 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 4381 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 4441 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 4501 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg 4561 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 4621 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 4681 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 4741 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 4801 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 4861 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 4921 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 4981 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 5041 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 5101 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag 5161 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg 5221 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact 5281 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc 5341 ccacc //