LOCUS       V023787                 7916 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023787
VERSION     V023787
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3481
                     /label="HSPH1(NM_006644)"
                     /note="HSPH1(NM_006644)"
                     /gene="HSPH1"
     CDS             3497..3538
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     CDS             3545..3568
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     CDS             3608..3631
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     polyA_signal    3678..3902
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3948..4376
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4390..4719
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4786..5385
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    5559..5680
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5729..5745)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5753..5769
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5777..5807)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5822..5843
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6131..6716)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6887..7747)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7748..7852)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tcggtggtgg ggttggacgt gggctcgcag agctgctaca tcgcggtagc
      961 ccgggccggg ggcatcgaga ccatcgccaa tgagttcagc gaccggtgca ccccgtcagt
     1021 catatcattt ggatcaaaaa atagaacaat cggagttgca gccaaaaatc agcaaatcac
     1081 tcatgcaaac aatacggtgt ctaacttcaa aagatttcat ggccgagcat tcaatgaccc
     1141 cttcattcaa aaggagaagg aaaacttgag ttacgatttg gttccattga aaaatggtgg
     1201 agttggaata aaggtaatgt acatgggtga agaacatcta tttagtgtgg agcagataac
     1261 agccatgttg ttgactaagc tgaaggaaac tgctgaaaac agcctcaaga aaccagtaac
     1321 agattgtgtt atttcagtcc cctccttctt tacagatgct gagaggcgat ctgtgttaga
     1381 tgctgcacag attgttggcc taaactgttt aagacttatg aatgacatga cagctgttgc
     1441 tttgaattac ggaatttata agcaggatct cccaagcctg gatgagaaac ctcggatagt
     1501 ggtttttgtt gatatgggac attcagcttt tcaagtgtct gcttgtgctt ttaacaaggg
     1561 aaaattgaag gtactgggaa cagcttttga tcctttctta ggaggaaaaa acttcgatga
     1621 aaagttagtg gaacattttt gtgcagaatt taaaactaag tacaagttgg atgcaaaatc
     1681 caaaatacga gcactcctac gtctgtatca ggaatgtgaa aaactgaaaa agctaatgag
     1741 ctctaacagc acagaccttc cactgaatat cgaatgcttt atgaatgata aagatgtttc
     1801 cggaaagatg aacaggtcac aatttgaaga actctgtgct gaacttctgc aaaagataga
     1861 agtacccctt tattcactgt tggaacaaac tcatctcaaa gtagaagatg tgagtgcagt
     1921 tgagattgtt ggaggcgcta cacgaattcc agctgtgaag gaaagaattg ccaaattctt
     1981 tggaaaagat attagcacaa cactcaatgc agatgaagca gtagccagag gatgtgcatt
     2041 acagtgtgca atactttccc cggcatttaa agttagagaa ttttccgtca cagatgcagt
     2101 tccttttcca atatctctga tctggaacca tgattcagaa gatactgaag gtgttcatga
     2161 agtctttagt cgaaaccatg ctgctccttt ctccaaagtt ctcacctttc tgagaagggg
     2221 gccttttgag ctagaagctt tctattctga tccccaagga gttccatatc cagaagcaaa
     2281 aataggccgc tttgtagttc agaatgtttc tgcacagaaa gatggagaaa aatctagagt
     2341 aaaagtcaaa gtgcgagtca acacccatgg cattttcacc atctctacgg catctatggt
     2401 ggagaaagtc ccaactgagg agaatgaaat gtcttctgaa gctgacatgg agtgtctgaa
     2461 tcagagacca ccagaaaacc cagacactga taaaaatgtc cagcaagaca acagtgaagc
     2521 tggaacacag ccccaggtac aaactgatgc tcaacaaacc tcacagtctc ccccttcacc
     2581 tgaacttacc tcagaagaaa acaaaatccc agatgctgac aaagcaaatg aaaaaaaagt
     2641 tgaccagcct ccagaagcta aaaagcccaa aataaaggtg gtgaatgttg agctgcctat
     2701 tgaagccaac ttggtctggc agttagggaa agaccttctt aacatgtata ttgagacaga
     2761 gggtaagatg ataatgcaag ataaattgga aaaagaaagg aatgatgcta aaaatgcagt
     2821 tgaggaatat gtgtatgagt tcagagacaa gctgtgtgga ccatatgaaa aatttatatg
     2881 tgagcaggat catcaaaatt ttttgagact cctcacagaa actgaagact ggctgtatga
     2941 agaaggagag gaccaagcta aacaagcata tgttgacaag ttggaagaat taatgaaaat
     3001 tggcactcca gttaaagttc ggtttcagga agctgaagaa cggccaaaaa tgtttgaaga
     3061 actaggacag aggctgcagc attatgccaa gatagcagct gacttcagaa ataaggatga
     3121 gaaatacaac catattgatg agtctgaaat gaaaaaagtg gagaagtctg ttaatgaagt
     3181 gatggaatgg atgaataatg tcatgaatgc tcaggctaaa aagagtcttg atcaggatcc
     3241 agttgtacgt gctcaggaaa ttaaaacaaa aatcaaggaa ttgaacaaca catgtgaacc
     3301 cgttgtaaca caaccgaaac caaaaattga atcacccaaa ctggaaagaa ctccaaatgg
     3361 cccaaatatt gataaaaagg aagaagattt agaagacaaa aacaattttg gtgctgaacc
     3421 tccacatcag aatggtgaat gttaccctaa tgagaaaaat tctgttaata tggacttgga
     3481 cgcggccgcg ggtagcggta agcctatccc taaccctctc ctcggtctcg attctacggg
     3541 tagctggagc catccgcagt ttgaaaaagg tggtggtagc ggtggtggtt caggtggtag
     3601 cagtgcatgg tcacaccctc agtttgagaa atgactcgag tctagagggc ccgtttaaac
     3661 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3721 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3781 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3841 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3901 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3961 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4021 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4081 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4141 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4201 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4261 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4321 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4621 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4741 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgac cgagtacaag
     4801 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     4861 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     4921 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     4981 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5041 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5101 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5161 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5221 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5281 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5341 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagcggg actctggggt
     5401 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5461 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5521 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5581 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5641 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5701 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5761 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5821 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5881 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5941 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6001 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6061 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6541 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6661 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7861 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggt
//
