LOCUS V032416 8642 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032416 VERSION V032416 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..4261 /label="DSG2(NM_001943)" /note="DSG2(NM_001943)" /gene="DSG2" CDS 4268..4357 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 4404..4628 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4674..5102 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5116..5445 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5512..6111 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 6285..6406 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6455..6471) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6479..6495 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6503..6533) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6548..6569 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6857..7442) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7613..8473) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8474..8578) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gcgcggagcc cgggacgcgc gtacgccctg ctgcttctcc tgatctgctt 961 taacgttgga agtggacttc acttacaggt cttaagcaca agaaatgaaa ataagctgct 1021 tcctaaacat cctcatttag tgcggcaaaa gcgcgcctgg atcaccgccc ccgtggctct 1081 tcgggaggga gaggatctgt ccaagaagaa tccaattgcc aagatacatt ctgatcttgc 1141 agaagaaaga ggactcaaaa ttacttacaa atacactgga aaagggatta cagagccacc 1201 ttttggtata tttgtcttta acaaagatac tggagaactg aatgttacca gcattcttga 1261 tcgagaagaa acaccatttt ttctgctaac aggttacgct ttggatgcaa gaggaaacaa 1321 tgtagagaaa cccttagagc tacgcattaa ggttcttgat atcaatgaca acgaaccagt 1381 gttcacacag gatgtctttg ttgggtctgt tgaagagttg agtgcagcac atactcttgt 1441 gatgaaaatc aatgcaacag atgcagatga gcccaatacc ctgaattcga aaatttccta 1501 tagaatcgta tctctggagc ctgcttatcc tccagtgttc tacctaaata aagatacagg 1561 agagatttat acaaccagtg ttaccttgga cagagaggaa cacagcagct acactttgac 1621 agtagaagca agagatggca atggagaagt tacagacaaa cctgtaaaac aagctcaagt 1681 tcagattcgt attttggatg tcaatgacaa tatacctgta gtagaaaata aagtgcttga 1741 agggatggtt gaagaaaatc aagtcaacgt agaagttacg cgcataaaag tgttcgatgc 1801 agatgaaata ggttctgata attggctggc aaattttaca tttgcatcag gaaatgaagg 1861 aggttatttc cacatagaaa cagatgctca aactaacgaa ggaattgtga cccttattaa 1921 ggaagtagat tatgaagaaa tgaagaatct tgacttcagt gttattgtcg ctaataaagc 1981 agcttttcac aagtcgatta ggagtaaata caagcctaca cccattccca tcaaggtcaa 2041 agtgaaaaat gtgaaagaag gcattcattt taaaagcagc gtcatctcaa tttatgttag 2101 cgagagcatg gatagatcaa gcaaaggcca aataattgga aattttcaag cttttgatga 2161 ggacactgga ctaccagccc atgcaagata tgtaaaatta gaagatagag ataattggat 2221 ctctgtggat tctgtcacat ctgaaattaa acttgcaaaa cttcctgatt ttgaatctag 2281 atatgttcaa aatggcacat acactgtaaa gattgtggcc atatcagaag attatcctag 2341 aaaaaccatc actggcacag tccttatcaa tgttgaagac atcaacgaca actgtcccac 2401 actgatagag cctgtgcaga caatctgtca cgatgcagag tatgtgaatg ttactgcaga 2461 ggacctggat ggacacccaa acagtggccc tttcagtttc tccgtcattg acaaaccacc 2521 tggcatggca gaaaaatgga aaatagcacg ccaagaaagt accagtgtgc tgctgcaaca 2581 aagtgagaaa aagcttggga gaagtgaaat tcagttcctg atttcagaca atcagggttt 2641 tagttgtcct gaaaagcagg tccttacact cacagtttgt gagtgtctgc atggcagcgg 2701 ctgcagggaa gcacagcatg actcctatgt gggcctggga cccgcagcaa ttgcgctcat 2761 gattttggcc tttctgctcc tgctattggt accactttta ctgctgatgt gccattgcgg 2821 aaagggcgcc aaaggcttta cccccatacc tggcaccata gagatgctgc atccttggaa 2881 taatgaagga gcaccacctg aagacaaggt ggtgccatca tttctgccag tggatcaagg 2941 gggcagtcta gtaggaagaa atggagtagg aggtatggcc aaggaagcca cgatgaaagg 3001 aagtagctct gcttccattg tcaaagggca acatgagatg tccgagatgg atggaaggtg 3061 ggaagaacac agaagcctgc tttctggtag agctacccag tttacagggg ccacaggcgc 3121 tatcatgacc actgaaacca cgaagaccgc aagggccaca ggggcttcca gagacatggc 3181 cggagctcag gcagctgctg ttgcactgaa cgaagaattc ttaagaaatt atttcactga 3241 taaagcggcc tcttacactg aggaagatga aaatcacaca gccaaagatt gccttctggt 3301 ttattctcag gaagaaactg aatcgctgaa tgcttctatt ggttgttgca gttttattga 3361 aggagagcta gatgaccgct tcttagatga tttgggactt aaattcaaga cactagctga 3421 agtttgcctg ggtcaaaaaa tagatataaa taaggaaatt gagcagagac aaaaacctgc 3481 cacagaaaca agtatgaaca cagcttcaca ttcactctgt gagcaaacta tggttaattc 3541 agagaatacc tactcctctg gcagtagctt cccagttcca aaatctttgc aagaagccaa 3601 tgcagagaaa gtaactcagg aaatagtcac tgaaagatct gtgtcttcta ggcaggcgca 3661 aaaggtagct acacctcttc ctgacccaat ggcttctaga aatgtgatag caacagaaac 3721 ttcctatgtc acagggtcca ctatgccacc aaccactgtg atcctgggtc ctagccagcc 3781 acagagcctt attgtgacag agagggtgta tgctccagct tctaccttgg tagatcagcc 3841 ttatgctaat gaaggtacag ttgtggtcac tgaaagagta atacagcctc atgggggtgg 3901 atcgaatcct ctggaaggca ctcagcatct tcaagatgta ccttacgtca tggtgaggga 3961 aagagagagc ttccttgccc ccagctcagg tgtgcagcct actctggcca tgcctaatat 4021 agcagtagga cagaatgtga cagtgacaga aagagttcta gcacctgctt ccactctgca 4081 atccagttac cagattccca ctgaaaattc tatgacggct aggaacacca cggtgtctgg 4141 agctggagtc cctggccctc tgccagattt tggtttagag gaatctggtc attctaattc 4201 taccataacc acatcttcca ccagagttac caagcatagc actgtacagc attcttactc 4261 cgatatcgaa caaaaactaa tatcagagga agatttggaa cagaaactaa tcagtgaaga 4321 agatttagag caaaagttaa tttccgagga ggacttatag ctcgagtcta gagggcccgt 4381 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 4441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 4501 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 4561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 4621 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 4681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 4741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 4801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 4861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 4921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 4981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 5041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 5101 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 5161 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 5221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 5281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 5341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 5401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 5461 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgaccgag 5521 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc 5581 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc 5641 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5701 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5761 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5821 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 5881 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 5941 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6001 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6061 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agcgggactc 6121 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 6181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 6241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 6301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 6361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7381 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 7441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 7501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 7561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 7621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 7681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 7741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 7801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 7861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 7921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 7981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 8041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 8101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 8161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 8221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 8281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 8341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 8401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 8461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 8521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 8581 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 8641 gt //