LOCUS V038804 9788 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038804 VERSION V038804 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..4747 /label="ABCB1(NM_001348946)" /note="ABCB1(NM_001348946)" /gene="ABCB1" CDS 4796..5506 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 5550..5774 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 5820..6248 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 6262..6591 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6658..7257 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 7431..7552 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7601..7617) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7625..7641 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7649..7679) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7694..7715 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(8003..8588) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8759..9619) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9620..9724) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gatcttgaag gggaccgcaa tggaggagca aagaagaaga acttttttaa 961 actgaacaat aaaagtgaaa aagataagaa ggaaaagaaa ccaactgtca gtgtattttc 1021 aatgtttcgc tattcaaatt ggcttgacaa gttgtatatg gtggtgggaa ctttggctgc 1081 catcatccat ggggctggac ttcctctcat gatgctggtg tttggagaaa tgacagatat 1141 ctttgcaaat gcaggaaatt tagaagatct gatgtcaaac atcactaata gaagtgatat 1201 caatgataca gggttcttca tgaatctgga ggaagacatg accaggtatg cctattatta 1261 cagtggaatt ggtgctgggg tgctggttgc tgcttacatt caggtttcat tttggtgcct 1321 ggcagctgga agacaaatac acaaaattag aaaacagttt tttcatgcta taatgcgaca 1381 ggagataggc tggtttgatg tgcacgatgt tggggagctt aacacccgac ttacagatga 1441 tgtctccaag attaatgaag gaattggtga caaaattgga atgttctttc agtcaatggc 1501 aacatttttc actgggttta tagtaggatt tacacgtggt tggaagctaa cccttgtgat 1561 tttggccatc agtcctgttc ttggactgtc agctgctgtc tgggcaaaga tactatcttc 1621 atttactgat aaagaactct tagcgtatgc aaaagctgga gcagtagctg aagaggtctt 1681 ggcagcaatt agaactgtga ttgcatttgg aggacaaaag aaagaacttg aaaggtacaa 1741 caaaaattta gaagaagcta aaagaattgg gataaagaaa gctattacag ccaatatttc 1801 tataggtgct gctttcctgc tgatctatgc atcttatgct ctggccttct ggtatgggac 1861 caccttggtc ctctcagggg aatattctat tggacaagta ctcactgtat tcttttctgt 1921 attaattggg gcttttagtg ttggacaggc atctccaagc attgaagcat ttgcaaatgc 1981 aagaggagca gcttatgaaa tcttcaagat aattgataat aagccaagta ttgacagcta 2041 ttcgaagagt gggcacaaac cagataatat taagggaaat ttggaattca gaaatgttca 2101 cttcagttac ccatctcgaa aagaagttaa gatcttgaag ggtctgaacc tgaaggtgca 2161 gagtgggcag acggtggccc tggttggaaa cagtggctgt gggaagagca caacagtcca 2221 gctgatgcag aggctctatg accccacaga ggggatggtc agtgttgatg gacaggatat 2281 taggaccata aatgtaaggt ttctacggga aatcattggt gtggtgagtc aggaacctgt 2341 attgtttgcc accacgatag ctgaaaacat tcgctatggc cgtgaaaatg tcaccatgga 2401 tgagattgag aaagctgtca aggaagccaa tgcctatgac tttatcatga aactgcctca 2461 taaatttgac accctggttg gagagagagg ggcccagttg agtggtgggc agaagcagag 2521 gatcgccatt gcacgtgccc tggttcgcaa ccccaagatc ctcctgctgg atgaggccac 2581 gtcagccttg gacacagaaa gcgaagcagt ggttcaggtg gctctggata aggccagaaa 2641 aggtcggacc accattgtga tagctcatcg tttgtctaca gttcgtaatg ctgacgtcat 2701 cgctggtttc gatgatggag tcattgtgga gaaaggaaat catgatgaac tcatgaaaga 2761 gaaaggcatt tacttcaaac ttgtcacaat gcagacagca ggaaatgaag ttgaattaga 2821 aaatgcagct gatgaatcca aaagtgaaat tgatgccttg gaaatgtctt caaatgattc 2881 aagatccagt ctaataagaa aaagatcaac tcgtaggagt gtccgtggat cacaagccca 2941 agacagaaag cttagtacca aagaggctct ggatgaaagt atacctccag tttccttttg 3001 gaggattatg aagctaaatt taactgaatg gccttatttt gttgttggtg tattttgtgc 3061 cattataaat ggaggcctgc aaccagcatt tgcaataata ttttcaaaga ttataggggt 3121 ttttacaaga attgatgatc ctgaaacaaa acgacagaat agtaacttgt tttcactatt 3181 gtttctagcc cttggaatta tttcttttat tacatttttc cttcagggtt tcacatttgg 3241 caaagctgga gagatcctca ccaagcggct ccgatacatg gttttccgat ccatgctcag 3301 acaggatgtg agttggtttg atgaccctaa aaacaccact ggagcattga ctaccaggct 3361 cgccaatgat gctgctcaag ttaaaggggc tataggttcc aggcttgctg taattaccca 3421 gaatatagca aatcttggga caggaataat tatatccttc atctatggtt ggcaactaac 3481 actgttactc ttagcaattg tacccatcat tgcaatagca ggagttgttg aaatgaaaat 3541 gttgtctgga caagcactga aagataagaa agaactagaa ggttctggga agatcgctac 3601 tgaagcaata gaaaacttcc gaaccgttgt ttctttgact caggagcaga agtttgaaca 3661 tatgtatgct cagagtttgc aggtaccata cagaaactct ttgaggaaag cacacatctt 3721 tggaattaca ttttccttca cccaggcaat gatgtatttt tcctatgctg gatgtttccg 3781 gtttggagcc tacttggtgg cacataaact catgagcttt gaggatgttc tgttagtatt 3841 ttcagctgtt gtctttggtg ccatggccgt ggggcaagtc agttcatttg ctcctgacta 3901 tgccaaagcc aaaatatcag cagcccacat catcatgatc attgaaaaaa cccctttgat 3961 tgacagctac agcacggaag gcctaatgcc gaacacattg gaaggaaatg tcacatttgg 4021 tgaagttgta ttcaactatc ccacccgacc ggacatccca gtgcttcagg gactgagcct 4081 ggaggtgaag aagggccaga cgctggctct ggtgggcagc agtggctgtg ggaagagcac 4141 agtggtccag ctcctggagc ggttctacga ccccttggca gggaaagtgc tgcttgatgg 4201 caaagaaata aagcgactga atgttcagtg gctccgagca cacctgggca tcgtgtccca 4261 ggagcccatc ctgtttgact gcagcattgc tgagaacatt gcctatggag acaacagccg 4321 ggtggtgtca caggaagaga ttgtgagggc agcaaaggag gccaacatac atgccttcat 4381 cgagtcactg cctaataaat atagcactaa agtaggagac aaaggaactc agctctctgg 4441 tggccagaaa caacgcattg ccatagctcg tgcccttgtt agacagcctc atattttgct 4501 tttggatgaa gccacgtcag ctctggatac agaaagtgaa aaggttgtcc aagaagccct 4561 ggacaaagcc agagaaggcc gcacctgcat tgtgattgct caccgcctgt ccaccatcca 4621 gaatgcagac ttaatagtgg tgtttcagaa tggcagagtc aaggagcatg gcacgcatca 4681 gcagctgctg gcacagaaag gcatctattt ttcaatggtc agtgtccagg ctggaacaaa 4741 gcgccaggaa ttcgtcatgg atatcggtgg aggcggatca ggaggcggtg gatctatggt 4801 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca 4861 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc 4921 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc 4981 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc 5041 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat 5101 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga 5161 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca 5221 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct 5281 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt 5341 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat 5401 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc 5461 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagctcg agtctagagg 5521 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 5581 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 5641 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 5701 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 5761 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 5821 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 5881 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 5941 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 6001 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 6061 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 6121 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 6181 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 6241 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 6301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 6361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 6421 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 6481 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 6541 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 6601 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 6661 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc 6721 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc 6781 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 6841 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 6901 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 6961 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 7021 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 7081 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 7141 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 7201 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg 7261 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 7321 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 7381 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 7561 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 7621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 8101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8521 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 8581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 8641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 8701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 8761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 8821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 9001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 9061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 9121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 9181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 9241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 9301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 9361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 9421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 9481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 9541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 9601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 9661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 9721 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 9781 cctatggt //