LOCUS V028135 6123 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028135 VERSION V028135 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1384..2778 /label="WASHC1(NM_182905)" /note="WASHC1(NM_182905)" /gene="WASHC1" polyA_signal 2911..3032 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3039..3494) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3521..3625 /gene="bla" /label="AmpR promoter" promoter 3627..3984 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4019..4813 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5045..5092 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5421..6009 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag 1381 cttatgactc ctgtgaggat gcagcactcc ctggcaggtc agacctatgc cgtgcccttc 1441 atccagccag acctgcggcg agaggaggcc gtccagcaga tggcggatgc cctgcagtac 1501 ctgcagaagg tctctggaga catcttcagc aggatctccc agcaggtaga gcagagccgg 1561 agccaggtgc aggccattgg agagaaggtc tccttggccc aggccaagat tgagaagatc 1621 aagggcagca agaaggccat caaggtgttc tccagtgcca agtaccctgc tccagggcgc 1681 ctgcaggaat atggctccat cttcacgggc gcccaggacc ctggcctgca gagacgcccc 1741 cgccacagga tccagagcaa gcaccgcccc ctggacgagc gggccctgca ggagaagctg 1801 aaggactttc ctgtgtgcgt gagcaccaag ccggagcccg aggacgatgc agaagaggga 1861 cttgggggtc ttcccagcaa catcagctct gtcagctcct tgctgctctt caacaccacc 1921 gagaacctgt acaagaagta tgtcttcctg gaccccctgg ctggtgctgt aacaaagacc 1981 catgtgatgc tgggggcaga gacagaggag aagctgtttg atgccccctt gtccatcagc 2041 aagagagagc agctggaaca gcaggtccca gagaactact tctatgtgcc agacctgggc 2101 caggtgcctg agatccatgt tccatcctac ctgcctgacc tgcccggcat tgccaacgac 2161 ctcatgtaca gtgccgacct gggccccggc attgccccct ctgcccctgg caccattcca 2221 gaactgccca ccttccacac tgaggtagcc gagcctctca aggtagacct acaagatggg 2281 gtactaacac cacccccacc gcccccacca ccacccccag ctcctgaggt gctggccagt 2341 gcacccccac tcccaccctc aaccgcggcc cctgtaggcc aaggcgccag gcaggacgac 2401 agcagcagca gcgcatctcc ttcagtccag ggagctccca gggaagtggt cgacccctcc 2461 ggtggctggg ccactctgct agagtccatc cgccaagctg ggggcatcgg caaggccaag 2521 ctgcgcagca tgaaggagcg aaagctggag aagcagcagc agaaggagca ggagcaagtg 2581 agagccacga gccaaggtgg gcacttgatg tcggatctct tcaacaagct ggtcatgagg 2641 cgcaagggca tctctgggaa aggacctggg gctggtgagg ggcccggagg agcctttgtc 2701 cgcgtgtcag actccatccc tcctttgccg ccaccgcagc agccacaggc agaggaggac 2761 gaggacgact gggaatcgta gggatccacc ggatctagat aactgatcat aatcagccat 2821 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2881 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3001 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 3061 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 3121 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 3181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 3241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 3301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 3361 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 3421 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 3481 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3601 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3961 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 4021 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4081 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4141 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4201 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4261 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4321 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4381 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4441 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4501 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 4561 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4621 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4681 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4741 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4801 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4861 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4921 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4981 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 5041 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 5101 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5161 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5221 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 5281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 5341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 6061 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 6121 cat //