LOCUS V034794 5717 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034794 VERSION V034794 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..1626 /label="OSTM1(NM_014028)" /note="OSTM1(NM_014028)" /gene="OSTM1" CDS 1663..2382 /codon_start=1 /note="mammalian codon-optimized" /product="enhanced blue variant of GFP (Ai et al., 2007)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKL TLKFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNG IKVNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMV LLEFRTAAGITLGMDELYK*" /label="EBFP2" polyA_signal 2505..2626 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2633..3088) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3115..3219 /gene="bla" /label="AmpR promoter" promoter 3221..3578 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3613..4407 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4639..4686 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5015..5603 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatggag ccgggcccga cagccgcgca gcggaggtgt 661 tcgttgccgc cgtggctgcc gctggggctg ctgctgtggt cggggctggc cctgggcgcg 721 ctccccttcg gcagcagtcc gcacagggtc ttccacgacc tcctgtcgga gcagcagttg 781 ctggaggtgg aggacttgtc cctgtccctc ctgcagggtg gagggctggg gcctctgtcg 841 ctgcccccgg acctgccgga tctggatcct gagtgccggg agctcctgct ggacttcgcc 901 aacagcagcg cagagctgac agggtgtctg gtgcgcagcg cccggcccgt gcgcctctgt 961 cagacctgct accccctctt ccaacaggtc gtcagcaaga tggacaacat cagccgagcc 1021 gcggggaata cttcagagag tcagagttgt gccagaagtc tcttaatggc agatagaatg 1081 caaatagttg tgattctctc agaatttttt aataccacat ggcaggaggc aaattgtgca 1141 aattgtttaa caaacaacag tgaagaatta tcaaacagca cagtatattt ccttaatcta 1201 tttaatcaca ccctgacctg ctttgaacat aaccttcagg ggaatgcaca tagtctttta 1261 cagacaaaaa attattcaga agtatgcaaa aactgccgtg aagcatacaa aactctgagt 1321 agtctgtaca gtgaaatgca aaaaatgaat gaacttgaga ataaggctga acctggaaca 1381 catttatgca ttgatgtgga agatgcaatg aacatcactc gaaaactatg gagtcgaact 1441 ttcaactgtt cagtcccttg cagtgacaca gtgcctgtaa ttgctgtttc tgtgttcatt 1501 ctctttctac ctgttgtctt ctaccttagt agctttcttc actcagagca aaagaaacgc 1561 aaactcattc tgcccaaacg tctcaagtcc agtaccagtt ttgcaaatat tcaggaaaat 1621 tcaaacggat ccggcggcgg agggagtgga ggagggggaa gcatggtgag caagggcgag 1681 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 1741 aagttcagcg tgaggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag 1801 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgagc 1861 cacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 1921 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcacc 1981 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 2041 aagggcgtcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc 2101 aacagccaca acatctatat catggccgtc aagcagaaga acggcatcaa ggtgaacttc 2161 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 2221 acccccatcg gcgacggccc cgtgctgctg cccgacagcc actacctgag cacccagtcc 2281 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttccgcacc 2341 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga 2401 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 2461 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 2521 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 2581 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta 2641 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 2701 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 2761 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 2821 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 2881 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 2941 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 3001 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 3061 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 3121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3181 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc 3241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 3301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 3361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 3421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3481 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 3541 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga 3601 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 3661 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 3721 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 3781 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 3841 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 3901 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 3961 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 4021 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 4081 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 4141 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 4201 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 4261 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 4321 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 4381 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 4441 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 4501 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 4561 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg 4621 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt 4681 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac 4741 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg 4801 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt 4861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 4921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 4981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 5041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 5101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 5161 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 5221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 5281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 5341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 5401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 5461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 5521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 5581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 5641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 5701 gtattaccgc catgcat //