LOCUS V027138 6003 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027138 VERSION V027138 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1378..2658 /label="Mapk8(NM_001310453)" /note="Mapk8(NM_001310453)" /gene="Mapk8" polyA_signal 2791..2912 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2919..3374) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3401..3505 /gene="bla" /label="AmpR promoter" promoter 3507..3864 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3899..4693 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4925..4972 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5301..5889 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg 1381 agcagaagca aacgtgacaa caatttttat agtgtagaga ttggagattc tacattcaca 1441 gtcctaaaac gataccagaa tttaaagcct ataggctcag gagctcaagg aatagtgtgt 1501 gcagcttatg atgccattct tgaaagaaat gttgcaatca agaagctcag ccggccattt 1561 cagaatcaga cccatgctaa gcgcgcctac cgagaactag ttcttatgaa gtgtgttaat 1621 cacaaaaata taattggcct tttgaatgtt ttcacaccac agaaatccct agaagaattt 1681 caagatgttt acatagtcat ggagctcatg gatgcaaatc tttgccaagt gattcagatg 1741 gagttagatc atgaaagaat gtcctacctt ctctatcaaa tgctgtgtgg aatcaagcac 1801 cttcactctg ctggaattat tcatcgggac ttaaagccta gtaatatagt agtcaaatca 1861 gactgcactt tgaagattct tgattttgga ctggcgagga ctgcaggaac gagttttatg 1921 atgacgcctt atgtggtgac tcgctactac agagcaccag aggtcattct cggcatgggc 1981 tacaaggaga acgttgacat ttggtcagtt gggtgcatca tgggagaaat gatcaaaggt 2041 ggtgttttgt tcccaggtac agatcatatt gatcagtgga ataaagttat tgaacagctc 2101 ggaacacctt gtcctgaatt catgaagaaa ctacaaccaa cagtaaggac ttatgttgaa 2161 aacaggccta aatacgctgg atatagcttt gagaaactgt tccccgatgt gcttttccca 2221 gctgactcag agcataacaa acttaaagcc agtcaggcaa gagatttgtt atccaaaatg 2281 ctagtaatag atgcatccaa aaggatctcc gtagatgaag ctctccagca cccatacatc 2341 aacgtctggt atgatccttc agaagcagaa gccccaccac caaagatccc ggacaagcag 2401 ttagatgaga gggagcacac aatagaggag tggaaagaac tgatatacaa ggaggtaatg 2461 gatttggagg aacgaactaa gaatggagtc ataagagggc agccgtctcc tttaggtgca 2521 gcaatgatca atggctctca gcatccatcg tcttcgccgt ctgtcaatga catgtcttca 2581 atgtccacag atccgacttt ggcctcggat acagacagca gtctagaagc atcagctgga 2641 cctctgggct gctgtagatg aggatccacc ggatctagat aactgatcat aatcagccat 2701 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2761 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2821 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2881 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 2941 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 3001 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 3061 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 3121 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 3181 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 3241 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 3301 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 3361 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3481 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3541 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3601 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3661 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3721 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3781 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3841 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 3901 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 3961 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4021 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4081 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4141 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4201 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4261 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4321 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4381 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 4441 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4501 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4561 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4621 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4681 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4741 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4801 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4861 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 4921 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 4981 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 5041 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 5101 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 6001 cat //