LOCUS V033720 6026 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033720 VERSION V033720 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1320 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1360..2589 /label="LAMP2(NM_002294)" /note="LAMP2(NM_002294)" /gene="LAMP2" CDS 2596..2622 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2626..2652 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2656..2682 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 2814..2935 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2942..3397) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3424..3528 /gene="bla" /label="AmpR promoter" promoter 3530..3887 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3922..4716 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4948..4995 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5324..5912 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctcgagg aggaggggga agcgaagcta tggtgtgctt ccgcctcttc 1381 ccggttccgg gctcagggct cgttctggtc tgcctagtcc tgggagctgt gcggtcttat 1441 gcattggaac ttaatttgac agattcagaa aatgccactt gcctttatgc aaaatggcag 1501 atgaatttca cagtacgcta tgaaactaca aataaaactt ataaaactgt aaccatttca 1561 gaccatggca ctgtgacata taatggaagc atttgtgggg atgatcagaa tggtcccaaa 1621 atagcagtgc agttcggacc tggcttttcc tggattgcga attttaccaa ggcagcatct 1681 acttattcaa ttgacagcgt ctcattttcc tacaacactg gtgataacac aacatttcct 1741 gatgctgaag ataaaggaat tcttactgtt gatgaacttt tggccatcag aattccattg 1801 aatgaccttt ttagatgcaa tagtttatca actttggaaa agaatgatgt tgtccaacac 1861 tactgggatg ttcttgtaca agcttttgtc caaaatggca cagtgagcac aaatgagttc 1921 ctgtgtgata aagacaaaac ttcaacagtg gcacccacca tacacaccac tgtgccatct 1981 cctactacaa cacctactcc aaaggaaaaa ccagaagctg gaacctattc agttaataat 2041 ggcaatgata cttgtctgct ggctaccatg gggctgcagc tgaacatcac tcaggataag 2101 gttgcttcag ttattaacat caaccccaat acaactcact ccacaggcag ctgccgttct 2161 cacactgctc tacttagact caatagcagc accattaagt atctagactt tgtctttgct 2221 gtgaaaaatg aaaaccgatt ttatctgaag gaagtgaaca tcagcatgta tttggttaat 2281 ggctccgttt tcagcattgc aaataacaat ctcagctact gggatgcccc cctgggaagt 2341 tcttatatgt gcaacaaaga gcagactgtt tcagtgtctg gagcatttca gataaatacc 2401 tttgatctaa gggttcagcc tttcaatgtg acacaaggaa agtattctac agctcaagac 2461 tgcagtgcag atgacgacaa cttccttgtg cccatagcgg tgggagctgc cttggcagga 2521 gtacttattc tagtgttgct ggcttatttt attggtctca agcaccatca tgctggatat 2581 gagcaatttg gatcctaccc atacgacgta cctgattatg caacttatcc ttatgatgta 2641 cctgattatg ctagctatcc ttatgatgtc ccagactacg catgagatcc accggatcta 2701 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 2761 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 2821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg 2941 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 3001 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 3061 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3121 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3181 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3241 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3301 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 3361 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 3421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 3541 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3661 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3721 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3781 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3841 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3901 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3961 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 4021 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 4081 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 4141 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4201 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4261 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4321 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4381 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4441 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4501 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4561 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4621 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4681 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4741 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4801 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4861 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4921 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4981 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 5041 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 5101 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 5161 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5221 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5461 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5941 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6001 tggataaccg tattaccgcc atgcat //