LOCUS V024130 5997 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024130 VERSION V024130 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 601..2574 /label="CPT2(NM_000098)" /note="CPT2(NM_000098)" /gene="CPT2" CDS 2581..2646 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2785..2906 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2913..3368) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3395..3499 /gene="bla" /label="AmpR promoter" promoter 3501..3858 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3893..4687 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4919..4966 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5295..5883 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 601 atggtgcccc gcctgctgct gcgcgcctgg ccccggggcc ccgcggttgg tccgggagcc 661 cccagtcggc ccctcagcgc cggctccggg cccggccagt acctgcagcg cagcatcgtg 721 cccaccatgc actaccagga cagcctgccc aggctgccta ttcccaaact tgaagacacc 781 attaggagat acctcagtgc acagaagcct ctcttgaatg atggccagtt caggaaaaca 841 gaacaatttt gcaagagttt tgaaaatggg attggaaaag aactgcatga gcagctggtt 901 gctctggaca aacagaataa acatacaagc tacatttcgg gaccctggtt tgatatgtac 961 ctatctgctc gagactccgt tgttctgaac tttaatccat ttatggcttt caatcctgac 1021 ccaaaatctg agtataatga ccagctcacc cgggcaacca acatgactgt ttctgccatc 1081 cggtttctga agacactccg ggctggcctt ctggagccag aagtgttcca cttgaaccct 1141 gcaaaaagtg acactatcac cttcaagaga ctcatacgct ttgtgccttc ctctctgtcc 1201 tggtatgggg cctacctggt caatgcgtat cccctggata tgtcccagta ttttcggctt 1261 ttcaactcaa ctcgtttacc caaacccagt cgggatgaac tcttcactga tgacaaggcc 1321 agacacctcc tggtcctaag gaaaggaaat ttttatatct ttgatgtcct ggatcaagat 1381 gggaacattg tgagcccctc ggaaatccag gcacatctga agtacattct ctcagacagc 1441 agccccgccc ccgagtttcc cctggcatac ctgaccagtg agaaccgaga catctgggca 1501 gagctcaggc agaagctgat gagtagtggc aatgaggaga gcctgaggaa agtggactcg 1561 gcagtgttct gtctctgcct agatgacttc cccattaagg accttgtcca cttgtcccac 1621 aatatgctgc atggggatgg cacaaaccgc tggtttgata aatcctttaa cctcattatc 1681 gccaaggatg gctctactgc cgtccacttt gagcactctt ggggtgatgg tgtggcagtg 1741 ctcagatttt ttaatgaagt atttaaagac agcactcaga cccctgccgt cactccacag 1801 agccagccag ctaccactga ctctactgtc acggtgcaga aactcaactt cgagctgact 1861 gatgccttaa agactggcat cacagctgct aaggaaaagt ttgatgccac catgaaaacc 1921 ctcactattg actgcgtcca gtttcagaga ggaggcaaag aattcctgaa gaagcaaaag 1981 ctgagccctg acgcagttgc ccagctggca ttccagatgg ccttcctgcg gcagtacggg 2041 cagacagtgg ccacctacga gtcctgtagc actgccgcat tcaagcacgg ccgcactgag 2101 accatccgcc cggcctccgt ctatacaaag aggtgctctg aggcctttgt cagggagccc 2161 tccaggcaca gtgctggtga gcttcagcag atgatggttg agtgctccaa gtaccatggc 2221 cagctgacca aagaagcagc aatgggccag ggctttgacc gacacttgtt tgctctgcgg 2281 catctggcag cagccaaagg gatcatcttg cctgagctct acctggaccc tgcatacggg 2341 cagataaacc acaatgtcct gtccacgagc acactgagca gcccagcagt gaaccttggg 2401 ggctttgccc ctgtggtctc tgatggcttt ggtgttgggt atgctgttca tgacaactgg 2461 ataggctgca atgtctcttc ctacccaggc cgcaatgccc gggagtttct ccaatgtgtg 2521 gagaaggcct tagaagacat gtttgatgcc ttagaaggca aatccatcaa aagtggatcc 2581 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat 2641 gacaagtgag ggcccgttta aacgcggccg cgactctaga tcataatcag ccataccaca 2701 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 2761 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 2821 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 2881 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 2941 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3001 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3121 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3361 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 3481 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 3541 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 3601 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3661 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 3721 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 3781 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 3841 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 3901 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 3961 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4021 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4081 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4141 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4201 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4261 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4321 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4381 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4441 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 4501 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 4561 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 4621 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 4681 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 4741 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 4801 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 4861 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 4921 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 4981 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5041 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5101 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 5821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 5881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 5941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //