LOCUS       V023914                11577 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023914
VERSION     V023914
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..8232
                     /label="ARID1A(NM_006015)"
                     /note="ARID1A(NM_006015)"
                     /gene="ARID1A"
     polyA_signal    8365..8486
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(8493..8948)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8975..9079
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        9081..9438
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             9473..10267
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    10499..10546
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      10875..11463
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 gccgcgcagg tcgcccccgc cgccgccagc agcctgggca acccgccgcc gccgccgccc
     1441 tcggagctga agaaagccga gcagcagcag cgggaggagg cggggggcga ggcggcggcg
     1501 gcggcagcgg ccgagcgcgg ggaaatgaag gcagccgccg ggcaggaaag cgagggcccc
     1561 gccgtggggc cgccgcagcc gctgggaaag gagctgcagg acggggccga gagcaatggg
     1621 ggtggcggcg gcggcggagc cggcagcggc ggcgggcccg gcgcggagcc ggacctgaag
     1681 aactcgaacg ggaacgcggg ccctaggccc gccctgaaca ataacctcac ggagccgccc
     1741 ggcggcggcg gtggcggcag cagcgatggg gtgggggcgc ctcctcactc agccgcggcc
     1801 gccttgccgc ccccagccta cggcttcggg caaccctacg gccggagccc gtctgccgtc
     1861 gccgccgccg cggccgccgt cttccaccaa caacatggcg gacaacaaag ccctggcctg
     1921 gcagcgctgc agagcggcgg cggcgggggc ctggagccct acgcggggcc ccagcagaac
     1981 tctcacgacc acggcttccc caaccaccag tacaactcct actaccccaa ccgcagcgcc
     2041 taccccccgc ccgccccggc ctacgcgctg agctccccga gaggtggcac tccgggctcc
     2101 ggcgcggcgg cggctgccgg ctccaagccg cctccctcct ccagcgcctc cgcctcctcg
     2161 tcgtcttcgt ccttcgctca gcagcgcttc ggggccatgg ggggaggcgg cccctccgcg
     2221 gccggcgggg gaactcccca gcccaccgcc acccccaccc tcaaccaact gctcacgtcg
     2281 cccagctcgg cccggggcta ccagggctac cccgggggcg actacagtgg cgggccccag
     2341 gacgggggcg ccggcaaggg cccggcggac atggcctcgc agtgttgggg ggctgcggcg
     2401 gcggcagctg cggcggcggc cgcctcggga ggggcccaac aaaggagcca ccacgcgccc
     2461 atgagccccg ggagcagcgg cggcgggggg cagccgctcg cccggacccc tcagccatcc
     2521 agtccaatgg atcagatggg caagatgaga cctcagccat atggcgggac taacccatac
     2581 tcgcagcaac agggacctcc gtcaggaccg cagcaaggac atgggtaccc agggcagcca
     2641 tacgggtccc agaccccgca gcggtacccg atgaccatgc agggccgggc gcagagtgcc
     2701 atgggcggcc tctcttatac acagcagatt cctccttatg gacaacaagg ccccagcggg
     2761 tatggtcaac agggccagac tccatattac aaccagcaaa gtcctcaccc tcagcagcag
     2821 cagccaccct actcccagca accaccgtcc cagacccctc atgcccaacc ttcgtatcag
     2881 cagcagccac agtctcaacc accacagctc cagtcctctc agcctccata ctcccagcag
     2941 ccatcccagc ctccacatca gcagtccccg gctccatacc cctcccagca gtcgacgaca
     3001 cagcagcacc cccagagcca gcccccctac tcacagccac aggctcagtc tccttaccag
     3061 cagcagcaac ctcagcagcc agcaccctcg acgctctccc agcaggctgc gtatcctcag
     3121 ccccagtctc agcagtccca gcaaactgcc tattcccagc agcgcttccc tccaccgcag
     3181 gagctatctc aagattcatt tgggtctcag gcatcctcag ccccctcaat gacctccagt
     3241 aagggagggc aagaagatat gaacctgagc cttcagtcaa gaccctccag cttgcctgat
     3301 ctatctggtt caatagatga cctccccatg gggacagaag gagctctgag tcctggagtg
     3361 agcacatcag ggatttccag cagccaagga gagcagagta atccagctca gtctcctttc
     3421 tctcctcata cctcccctca cctgcctggc atccgaggcc cttccccgtc ccctgttggc
     3481 tctcccgcca gtgttgctca gtctcgctca ggaccactct cgcctgctgc agtgccaggc
     3541 aaccagatgc cacctcggcc acccagtggc cagtcggaca gcatcatgca tccttccatg
     3601 aaccaatcaa gcattgccca agatcgaggt tatatgcaga ggaaccccca gatgccccag
     3661 tacagttccc cccagcccgg ctcagcctta tctccgcgtc agccttccgg aggacagata
     3721 cacacaggca tgggctccta ccagcagaac tccatgggga gctatggtcc ccaggggggt
     3781 cagtatggcc cacaaggtgg ctaccccagg cagccaaact ataatgcctt gcccaatgcc
     3841 aactacccca gtgcaggcat ggctggaggc ataaacccca tgggtgccgg aggtcaaatg
     3901 catggacagc ctggcatccc accttatggc acactccctc cagggaggat gagtcacgcc
     3961 tccatgggca accggcctta tggccctaac atggccaata tgccacctca ggttgggtca
     4021 gggatgtgtc ccccaccagg gggcatgaac cggaaaaccc aagaaactgc tgtcgccatg
     4081 catgttgctg ccaactctat ccaaaacagg ccgccaggct accccaatat gaatcaaggg
     4141 ggcatgatgg gaactggacc tccttatgga caagggatta atagtatggc tggcatgatc
     4201 aaccctcagg gacccccata ttccatgggt ggaaccatgg ccaacaattc tgcagggatg
     4261 gcagccagcc cagagatgat gggccttggg gatgtaaagt taactccagc caccaaaatg
     4321 aacaacaagg cagatgggac acccaagaca gaatccaaat ccaagaaatc cagttcttct
     4381 actacaacca atgagaagat caccaagttg tatgagctgg gtggtgagcc tgagaggaag
     4441 atgtgggtgg accgttatct ggccttcact gaggagaagg ccatgggcat gacaaatctg
     4501 cctgctgtgg gtaggaaacc tctggacctc tatcgcctct atgtgtctgt gaaggagatt
     4561 ggtggattga ctcaggtcaa caagaacaaa aaatggcggg aacttgcaac caacctcaat
     4621 gtgggcacat caagcagtgc tgccagctcc ttgaaaaagc agtatatcca gtgtctctat
     4681 gcctttgaat gcaagattga acggggagaa gaccctcccc cagacatctt tgcagctgct
     4741 gattccaaga agtcccagcc caagatccag cctccctctc ctgcgggatc aggatctatg
     4801 caggggcccc agactcccca gtcaaccagc agttccatgg cagaaggagg agacttaaag
     4861 ccaccaactc cagcatccac accacacagt cagatccccc cattgccagg catgagcagg
     4921 agcaattcag ttgggatcca ggatgccttt aatgatggaa gtgactccac attccagaag
     4981 cggaattcca tgactccaaa ccctgggtat cagcccagta tgaatacctc tgacatgatg
     5041 gggcgcatgt cctatgagcc aaataaggat ccttatggca gcatgaggaa agctccaggg
     5101 agtgatccct tcatgtcctc agggcagggc cccaacggcg ggatgggtga cccctacagt
     5161 cgtgctgccg gccctgggct aggaaatgtg gcgatgggac cacgacagca ctatccctat
     5221 ggaggtcctt atgacagagt gaggacggag cctggaatag ggcctgaggg aaacatgagc
     5281 actggggccc cacagccgaa tctcatgcct tccaacccag actcggggat gtattctcct
     5341 agccgctacc ccccgcagca gcagcagcag cagcagcaac gacatgattc ctatggcaat
     5401 cagttctcca cccaaggcac cccttctggc agccccttcc ccagccagca gactacaatg
     5461 tatcaacagc aacagcagaa ttacaagcgg ccaatggatg gcacatatgg ccctcctgcc
     5521 aagcggcacg aaggggagat gtacagcgtg ccatacagca ctgggcaggg gcagcctcag
     5581 cagcagcagt tgcccccagc ccagccccag cctgccagcc agcaacaagc tgcccagcct
     5641 tcccctcagc aagatgtata caaccagtat ggcaatgcct atcctgccac tgccacagct
     5701 gctactgagc gccgaccagc aggcggcccc cagaaccaat ttccattcca gtttggccga
     5761 gaccgtgtct ctgcaccccc tggcaccaat gcccagcaaa acatgccacc acaaatgatg
     5821 ggcggcccca tacaggcatc agctgaggtt gctcagcaag gcaccatgtg gcaggggcgt
     5881 aatgacatga cctataatta tgccaacagg cagagcacgg gctctgcccc ccagggcccc
     5941 gcctatcatg gcgtgaaccg aacagatgaa atgctgcaca cagatcagag ggccaaccac
     6001 gaaggctcgt ggccttccca tggcacacgc cagcccccat atggtccctc tgcccctgtg
     6061 ccccccatga caaggccccc tccatctaac taccagcccc caccaagcat gcagaatcac
     6121 attcctcagg tatccagccc tgctcccctg ccccggccaa tggagaaccg cacctctcct
     6181 agcaagtctc cattcctgca ctctgggatg aaaatgcaga aggcaggtcc cccagtacct
     6241 gcctcgcaca tagcacctgc ccctgtgcag ccccccatga ttcggcggga tatcaccttc
     6301 ccacctggct ctgttgaagc cacacagcct gtgttgaagc agaggaggcg gctcacaatg
     6361 aaagacattg gaaccccgga ggcatggcgg gtaatgatgt ccctcaagtc tggtctcctg
     6421 gcagagagca catgggcatt agataccatc aacatcctgc tgtatgatga caacagcatc
     6481 atgaccttca acctcagtca gctcccaggg ttgctagagc tccttgtaga atatttccga
     6541 cgatgcctga ttgagatctt tggcatttta aaggagtatg aggtgggtga cccaggacag
     6601 agaacgctac tggatcctgg gaggttcagc aaggtgtcta gtccagctcc catggagggt
     6661 ggggaagaag aagaagaact tctaggtcct aaactagaag aggaagaaga agaggaagta
     6721 gttgaaaatg atgaggagat agccttttca ggcaaggaca agccagcttc agagaatagt
     6781 gaggagaagc tgatcagtaa gtttgacaag cttccagtaa agatcgtaca gaagaatgat
     6841 ccatttgtgg tggactgctc agataagctt gggcgtgtgc aggagtttga cagtggcctg
     6901 ctgcactggc ggattggtgg gggggacacc actgagcata tccagaccca cttcgagagc
     6961 aagacagagc tgctgccttc ccggcctcac gcaccctgcc caccagcccc tcggaagcat
     7021 gtgacaacag cagagggtac accagggaca acagaccagg aggggccccc acctgatgga
     7081 cctccagaaa aacggatcac agccactatg gatgacatgt tgtctactcg gtctagcacc
     7141 ttgaccgagg atggagctaa gagttcagag gccatcaagg agagcagcaa gtttccattt
     7201 ggcattagcc cagcacagag ccaccggaac atcaagatcc tagaggacga accccacagt
     7261 aaggatgaga ccccactgtg tacccttctg gactggcagg attctcttgc caagcgctgc
     7321 gtctgtgtgt ccaataccat tcgaagcctg tcatttgtgc caggcaatga ctttgagatg
     7381 tccaaacacc cagggctgct gctcatcctg ggcaagctga tcctgctgca ccacaagcac
     7441 ccagaacgga agcaggcacc actaacttat gaaaaggagg aggaacagga ccaaggggtg
     7501 agctgcaaca aagtggagtg gtggtgggac tgcttggaga tgctccggga aaacaccttg
     7561 gttacactcg ccaacatctc ggggcagttg gacctatctc cataccccga gagcatttgc
     7621 ctgcctgtcc tggacggact cctacactgg gcagtttgcc cttcagctga agcccaggac
     7681 cccttttcca ccctgggccc caatgccgtc ctttccccgc agagactggt cttggaaacc
     7741 ctcagcaaac tcagcatcca ggacaacaat gtggacctga ttctggccac accccccttc
     7801 agccgcctgg agaagttgta tagcactatg gtgcgcttcc tcagtgaccg aaagaacccg
     7861 gtgtgccggg agatggctgt ggtactgctg gccaacctgg ctcaggggga cagcctggca
     7921 gctcgtgcca ttgcagtgca gaagggcagt atcggcaacc tcctgggctt cctagaggac
     7981 agccttgccg ccacacagtt ccagcagagc caggccagcc tcctccacat gcagaaccca
     8041 ccctttgagc caactagtgt ggacatgatg cggcgggctg cccgcgcgct gcttgccttg
     8101 gccaaggtgg acgagaacca ctcagagttt actctgtacg aatcacggct gttggacatc
     8161 tcggtatcac cgttgatgaa ctcattggtt tcacaagtca tttgtgatgt actgtttttg
     8221 attggccagt catgaggatc caccggatct agataactga tcataatcag ccataccaca
     8281 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8341 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8461 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     8521 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     8581 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     8641 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8701 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     8761 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8821 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8881 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8941 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     9001 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9061 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     9121 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9181 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9241 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9301 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9361 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9421 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     9481 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     9541 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     9601 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     9661 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     9721 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     9781 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     9841 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     9901 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     9961 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
    10021 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
    10081 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
    10141 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
    10201 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
    10261 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
    10321 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    10381 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
    10441 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
    10501 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
    10561 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
    10621 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
    10681 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
    10741 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10981 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    11041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    11101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
