LOCUS V021940 7326 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021940 VERSION V021940 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1393..3981 /label="ADAM15(NM_207197)" /note="ADAM15(NM_207197)" /gene="ADAM15" polyA_signal 4114..4235 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4242..4697) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4724..4828 /gene="bla" /label="AmpR promoter" promoter 4830..5187 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5222..6016 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6248..6295 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6624..7212 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag 1381 cttcgaattc tcatgcggct ggcgctgctc tgggccctgg ggctcctggg cgcgggcagc 1441 cctctgcctt cctggccgct cccaaatata ggtggcactg aggagcagca ggcagagtca 1501 gagaaggccc cgagggagcc cttggagccc caggtccttc aggacgatct cccaattagc 1561 ctcaaaaagg tgcttcagac cagtctgcct gagcccctga ggatcaagtt ggagctggac 1621 ggtgacagtc atatcctgga gctgctacag aatagggagt tggtcccagg ccgcccaacc 1681 ctggtgtggt accagcccga tggcactcgg gtggtcagtg agggacacac tttggagaac 1741 tgctgctacc agggaagagt gcggggatat gcaggctcct gggtgtccat ctgcacctgc 1801 tctgggctca gaggcttggt ggtcctgacc ccagagagaa gctataccct ggagcagggg 1861 cctggggacc ttcagggtcc tcccattatt tcgcgaatcc aagatctcca cctgccaggc 1921 cacacctgtg ccctgagctg gcgggaatct gtacacactc agaagccacc agagcacccc 1981 ctgggacagc gccacattcg ccggaggcgg gatgtggtaa cagagaccaa gactgtggag 2041 ttggtgattg tggctgatca ctcggaggcc cagaaatacc gggacttcca gcacctgcta 2101 aaccgcacac tggaagtggc cctcttgctg gacacattct tccggcccct gaatgtacga 2161 gtggcactag tgggcctgga ggcctggacc cagcgtgacc tggtggagat cagcccaaac 2221 ccagctgtca ccctcgaaaa cttcctccac tggcgcaggg cacatttgct gcctcgattg 2281 ccccatgaca gtgcccagct ggtgactggt acttcattct ctgggcctac ggtgggcatg 2341 gccattcaga actccatctg ttctcctgac ttctcaggag gtgtgaacat ggaccactcc 2401 accagcatcc tgggagtcgc ctcctccata gcccatgagt tgggccacag cctgggcctg 2461 gaccatgatt tgcctgggaa tagctgcccc tgtccaggtc cagccccagc caagacctgc 2521 atcatggagg cctccacaga cttcctacca ggcctgaact tcagcaactg cagccgacgg 2581 gccctggaga aagccctcct ggatggaatg ggcagctgcc tcttcgaacg gctgcctagc 2641 ctacccccta tggctgcttt ctgcggaaat atgtttgtgg agccgggcga gcagtgtgac 2701 tgtggcttcc tggatgactg cgtcgatccc tgctgtgatt ctttgacctg ccagctgagg 2761 ccaggtgcac agtgtgcatc tgacggaccc tgttgtcaaa attgccagct gcgcccgtct 2821 ggctggcagt gtcgtcctac cagaggggat tgtgacttgc ctgaattctg cccaggagac 2881 agctcccagt gtccccctga tgtcagccta ggggatggcg agccctgcgc tggcgggcaa 2941 gctgtgtgca tgcacgggcg ttgtgcctcc tatgcccagc agtgccagtc actttgggga 3001 cctggagccc agcccgctgc gccactttgc ctccagacag ctaatactcg gggaaatgct 3061 tttgggagct gtgggcgcaa ccccagtggc agttatgtgt cctgcacccc tagagatgcc 3121 atttgtgggc agctccagtg ccagacaggt aggacccagc ctctgctggg ctccatccgg 3181 gatctactct gggagacaat agatgtgaat gggactgagc tgaactgcag ctgggtgcac 3241 ctggacctgg gcagtgatgt ggcccagccc ctcctgactc tgcctggcac agcctgtggc 3301 cctggcctgg tgtgtataga ccatcgatgc cagcgtgtgg atctcctggg ggcacaggaa 3361 tgtcgaagca aatgccatgg acatggggtc tgtgacagca acaggcactg ctactgtgag 3421 gagggctggg caccccctga ctgcaccact cagctcaaag caaccagctc cctgaccaca 3481 gggctgctcc tcagcctcct ggtcttattg gtcctggtga tgcttggtgc cagctactgg 3541 taccgtgccc gcctgcacca gcgactctgc cagctcaagg gacccacctg ccagtacagg 3601 gcagcccaat ctggtccctc tgaacggcca ggacctccgc agagggccct gctggcacga 3661 ggcactaagc aggctagtgc tctcagcttc ccggcccccc cttccaggcc gctgccgcct 3721 gaccctgtgt ccaagagact ccaggctgag ctggctgacc gacccaatcc ccctacccgc 3781 cctctgcccg ctgacccggt ggtgagaagc ccgaagtctc aggggccagc caagccccca 3841 cccccaagga agccactgcc tgccgacccc cagggccggt gcccatcggg tgacctgccc 3901 ggcccagggg ctggaatccc gcccctagtg gtaccctcca gaccagcgcc accgcctccg 3961 acagtgtcct cgctctacct ctgaggatcc accggatcta gataactgat cataatcagc 4021 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 4081 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 4141 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 4201 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 4261 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 4321 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 4381 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 4441 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 4501 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 4561 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 4621 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 4681 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4741 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4801 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 4861 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4921 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 4981 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 5041 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5101 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 5161 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 5221 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 5281 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 5341 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 5401 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 5461 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 5521 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 5581 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 5641 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 5701 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 5761 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 5821 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 5881 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 5941 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 6001 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 6061 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 6121 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 6181 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 6241 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 6301 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 6361 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 6421 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 6481 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 6541 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 6601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 6661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 6721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 6781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 6841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 6901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 6961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 7021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 7081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 7141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 7201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 7261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 7321 atgcat //