LOCUS       pCMV-Myc-N.        3794 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for CMV promoter-driven expression of an ORF with 
            an N-terminal Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Myc-N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   transOMIC, Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             838..840
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             841..870
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    875..929
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(933..1067)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1178..1194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1202..1218)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1226..1256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1271..1292)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1580..2168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2342..3199)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3200..3304)
                     /label=AmpR promoter
     primer_bind     3778..3794
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattcggtcg
      901 accgagatct ctcgaggtac cgcggccgcg gggatccaga catgataaga tacattgatg
      961 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1021 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1081 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag
     1141 agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1321 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1381 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1441 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1501 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1561 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1621 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1681 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1741 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1801 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1861 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1921 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1981 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2041 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2101 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2161 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2221 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2281 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2341 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2401 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2461 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2521 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2581 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2641 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2701 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2761 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2821 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2881 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2941 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3001 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3061 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3121 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3181 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3241 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3301 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     3361 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     3421 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     3481 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     3541 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     3601 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     3661 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     3721 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     3781 aaacgacggc cagt
//
