LOCUS       .                       9128 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4695..4919
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4965..5393
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5407..5736
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5803..6594
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6771..6904
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6941..6957)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6965..6981)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6989..7019)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7034..7055)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7343..7928)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8102..8959)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8960..9064)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg actaacgaaa ctattgacca acaaccacaa accgaagcgg cttttaaccc
      961 gcagcaattt atcaacaatc ttcaagtggc ttttcttaaa gttgataacg ctgtcgcttc
     1021 atacgatcct gatcaaaaac caatcgttga taagaatgat agggataaca ggcaagcttt
     1081 tgatggaatc tcgcaattaa gggaagaata ctccaataaa gcgatcaaaa atcctaccaa
     1141 aaagaatcag tatttttcag actttatcaa taagagcaat gatttaatca acaaagacaa
     1201 tctcattgat ataggttctt ccataaaaag ctttcagaaa tttgggactc agcgttaccg
     1261 aattttcaca agttgggtgt cccatcaaaa cgatccgtct aaaatcaaca cccgatcgat
     1321 ccgaaatttt atggaaaata tcatacaacc ccctatccct gatgacaaag aaaaagcaga
     1381 gtttttgaaa tctgccaaac aatcttttgc aggaatcatt atagggaatc aaatccgaac
     1441 ggatcaaaag ttcatgggcg tgtttgatga attcttgaaa gaaaggcaag aagcagaaaa
     1501 aaatggagag cctactggtg gggattggtt ggatattttt ttatcatttg tatttaacaa
     1561 agaacaatct tctgatgtca aagaagcaat caatcaagaa ccagttcccc atgtccaacc
     1621 agatatagcc actaccacca cccacataca aggcttaccg cctgaatcta gggatttgct
     1681 tgatgaaagg ggtaattttt ctaaattcac tcttggcgat atggaaatgt tagatgttga
     1741 gggcgtcgcc gacattgatc ctaattacaa gttcaatcaa ttattgattc acaataacgc
     1801 tctgtcttct gtgttaatgg ggagtcataa tggcatagaa cctgaaaaag tttcattatt
     1861 gtatgcgggc aatggtggtt ttggagccaa gcacgattgg aacgccaccg ttggttataa
     1921 aaaccaacaa ggcgacaatg tggctacact cattaatgtg catatgaaaa acggcagtgg
     1981 cttagtcata gcaggtggtg agaaagggat taacaaccct agtttttatc tctacaaaga
     2041 agaccaactc acaggctcac aacgagcatt gagtcaagaa gagatccgaa acaaaataga
     2101 tttcatggaa tttcttgcac aaaacaatgc taaattagac aacttgagcg agaaagagaa
     2161 agaaaaattc caaaatgaga ttgaagattt tcaaaaagac tctaaggctt atttagacgc
     2221 cctagggaat gatcgtattg cctttgtttc taaaaaagac ccaaaacatt cagctttaat
     2281 tactgagttt ggtaaggggg atttgagcta cactctcaaa gattatggga aaaaagcaga
     2341 tagagcttta gatagggaga aaaatgttac tcttcaaggt aacctaaaac atgatagcgt
     2401 gatgtttgtt aattattcta atttcaaata caccaacgcc tccaagagtc ctgataaggg
     2461 tgtaggcgtt acaaatggcg tttcccattt agacgcaggc tttagcaagg tagctgtctt
     2521 taatttgcct gatttaaata atctcgctat cactagtttc gtaaggcgga atttagagaa
     2581 taaactagtc actgaaggat tgtccctaca agaagctaat aagcttatca aagatttttt
     2641 gagcagcaac aaagaattgg ttggaaaagc tttaaacttc aataaagctg tagctgacgc
     2701 taaaaacaca ggcaactatg atgaagtgaa aaaagctcag aaagatcttg aaaaatctct
     2761 aaggaaacga gagcatttag agaaagaagt agagaaaaaa ttggagagca aaagcggcaa
     2821 caaaaataaa atggaagcga aagctcaagc taacagccaa aaagataaga tttttgcgtt
     2881 gatcaataaa gaggctaata gggacgcaag agcaatcgct tactctcaga atcttaaagg
     2941 catcaaaagg gaattgtctg ataaacttga aaaaatcaac aaggatttga aagactttag
     3001 taaatctttt gatgaattca aaaatggcaa aaataaggat tttagcaagg cagaagaaac
     3061 gctaaaagcc cttaaaggct cggtgaaaga tttaggtatc aatccggaat ggatttcaaa
     3121 agttgaaaac cttaatgcag ctttgaatga attcaaaaat ggcaaaaata aggatttcag
     3181 caaggtaacg caagcaaaaa gcgaccttga aaattccgtt aaagatgtga tcatcaatca
     3241 aaagataacg gataaagttg acaatctcaa tcaagcggta tcaatggcta aagcaacggg
     3301 tgatttcagt agggtagagc aagcgttagc cgatctcaaa aacttctcaa aggagcaatt
     3361 ggctcaacaa actcaaaaaa atgaaagttt caatgttgga aaaaaatctg aaatatatca
     3421 atccgttaag aatggtgtga acggaaccct agtcggtaat gggttatctg gaatagaggc
     3481 cacagctctc gccaaaaatt tttcggatat caagaaagaa ttgaatgaga aatttaaaaa
     3541 tttcaataac aataacaata atggactcga aaacgaaccc atttatgcta aagttaataa
     3601 aaagaaaaca ggacaagtag ctagccctga agaacccatt tacgctcaag ttgctaaaaa
     3661 ggtgaatgca aaaattgacc gactcaatca agcagcaagt ggtttgggtg gtgtagggca
     3721 agcgggcttc cctttgaaaa ggcatgataa agttgatgat ctcagtaagg tagggcgatc
     3781 agttagccct gaacccattt atgctacgat tgatgatctc ggcggacctt tccctttgaa
     3841 aaggcatgat aaagttgatg atctcagtaa ggtagggcga tcagttagcc ctgaacccat
     3901 ttatgctacg attgatgatc tcggcggacc tttccctttg aaaaggcatg ataaagttga
     3961 tgatctcagt aaggtagggc gatcagttag ccctgaaccc atttatgcta cgattgatga
     4021 tctcggcgga cctttccctt tgaaaaggca tgataaagtt gatgatctca gtaaggtagg
     4081 gctttcaagg aatcaagaat tggctcagaa aattgacaat ctcagtcaag cggtatcaga
     4141 agctaaagca ggttttttta gcaatctaga gcaaacgata gacaagctca aagattctac
     4201 aaaatacaat tctgtgaatc tatgggttga aagtgcaaaa aaagtgcctg ctagtttgtc
     4261 agcgaaacta gacaattacg ctactaacag ccacacacgc attaatagca atatccaaaa
     4321 tggagcaatc aatgaaaaag cgaccggtat gctaacgcaa aaaaaccctg agtggctcaa
     4381 gctcgtgaat gataagatag ttgcgcataa tgtgggaagc gttcctttgt cagagtatga
     4441 taaaattggc ttcaaccaga agaatatgaa agattattct gattcgttca agttttccac
     4501 caagttgaac aatgctgtaa aagacgttaa gtctagcttt acgcaatttt tagccaatgc
     4561 attttctaca ggatattact ccttggcgag ggaaaatgcg gagcatggaa tcaaaaatgt
     4621 taatacaaaa ggtggtttcc aaaaatctta gctcgagtct agagggcccg tttaaacccg
     4681 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4741 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4801 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4861 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4921 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5161 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5281 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5641 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5701 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5761 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5821 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5881 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5941 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6001 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6061 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6121 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6181 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6241 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6301 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6361 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6421 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6481 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6541 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6601 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6661 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6721 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6901 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7921 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7981 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8041 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8101 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8161 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8221 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8281 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8341 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8401 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8461 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8521 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8581 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8641 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8701 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8761 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8821 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8881 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8941 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9001 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9061 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9121 cctatggt
//
