LOCUS       .                       9092 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             908..4585
                     /gene="ADAR"
                     /label="ADAR"
                     /note="Double-stranded RNA-specific adenosine deaminase
                     from Homo sapiens. Accession#: P55265"
     CDS             4595..4612
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    4659..4883
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4929..5357
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5371..5700
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5767..6558
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6735..6868
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6905..6921)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6929..6945)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6953..6983)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6998..7019)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7307..7892)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8066..8923)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8924..9028)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aatccgcggc aggggtattc cctcagcgga tactacaccc atccatttca
      961 aggctatgag cacagacagc tcagatacca gcagcctggg ccaggatctt cccccagtag
     1021 tttcctgctt aagcaaatag aatttctcaa ggggcagctc ccagaagcac cggtgattgg
     1081 aaagcagaca ccgtcactgc caccttccct cccaggactc cggccaaggt ttccagtact
     1141 acttgcctcc agtaccagag gcaggcaagt ggacatcagg ggtgtcccca ggggcgtgca
     1201 tctcggaagt caggggctcc agagagggtt ccagcatcct tcaccacgtg gcaggagtct
     1261 gccacagaga ggtgttgatt gcctttcctc acatttccag gaactgagta tctaccaaga
     1321 tcaggaacaa aggatcttaa agttcctgga agagcttggg gaagggaagg ccaccacagc
     1381 acatgatctg tctgggaaac ttgggactcc gaagaaagaa atcaatcgag ttttatactc
     1441 cctggcaaag aagggcaagc tacagaaaga ggcaggaaca ccccctttgt ggaaaatcgc
     1501 ggtctccact caggcttgga accagcacag cggagtggta agaccagacg gtcatagcca
     1561 aggagcccca aactcagacc cgagtttgga accggaagac agaaactcca catctgtctc
     1621 agaagatctt cttgagcctt ttattgcagt ctcagctcag gcttggaacc agcacagcgg
     1681 agtggtaaga ccagacagtc atagccaagg atccccaaac tcagacccag gtttggaacc
     1741 tgaagacagc aactccacat ctgccttgga agatcctctt gagtttttag acatggccga
     1801 gatcaaggag aaaatctgcg actatctctt caatgtgtct gactcctctg ccctgaattt
     1861 ggctaaaaat attggcctta ccaaggcccg agatataaat gctgtgctaa ttgacatgga
     1921 aaggcagggg gatgtctata gacaagggac aacccctccc atatggcatt tgacagacaa
     1981 gaagcgagag aggatgcaaa tcaagagaaa tacgaacagt gttcctgaaa ccgctccagc
     2041 tgcaatccct gagaccaaaa gaaacgcaga gttcctcacc tgtaatatac ccacatcaaa
     2101 tgcctcaaat aacatggtaa ccacagaaaa agtggagaat gggcaggaac ctgtcataaa
     2161 gttagaaaac aggcaagagg ccagaccaga accagcaaga ctgaaaccac ctgttcatta
     2221 caatggcccc tcaaaagcag ggtatgttga ctttgaaaat ggccagtggg ccacagatga
     2281 catcccagat gacttgaata gtatccgcgc agcaccaggt gagtttcgag ccatcatgga
     2341 gatgccctcc ttctacagtc atggcttgcc acggtgttca ccctacaaga aactgacaga
     2401 gtgccagctg aagaacccca tcagcgggct gttagaatat gcccagttcg ctagtcaaac
     2461 ctgtgagttc aacatgatag agcagagtgg accaccccat gaacctcgat ttaaattcca
     2521 ggttgtcatc aatggccgag agtttccccc agctgaagct ggaagcaaga aagtggccaa
     2581 gcaggatgca gctatgaaag ccatgacaat tctgctagag gaagccaaag ccaaggacag
     2641 tggaaaatca gaagaatcat cccactattc cacagagaaa gaatcagaga agactgcaga
     2701 gtcccagacc cccacccctt cagccacatc cttcttttct gggaagagcc ccgtcaccac
     2761 actgcttgag tgtatgcaca aattggggaa ctcctgcgaa ttccgtctcc tgtccaaaga
     2821 aggccctgcc catgaaccca agttccaata ctgtgttgca gtgggagccc aaactttccc
     2881 cagtgtgagt gctcccagca agaaagtggc aaagcagatg gccgcagagg aagccatgaa
     2941 ggccctgcat ggggaggcga ccaactccat ggcttctgat aaccagcctg aaggtatgat
     3001 ctcagagtca cttgataact tggaatccat gatgcccaac aaggtcagga agattggcga
     3061 gctcgtgaga tacctgaaca ccaaccctgt gggtggcctt ttggagtacg cccgctccca
     3121 tggctttgct gctgaattca agttggtcga ccagtccgga cctcctcacg agcccaagtt
     3181 cgtttaccaa gcaaaagttg ggggtcgctg gttcccagcc gtctgcgcac acagcaagaa
     3241 gcaaggcaag caggaagcag cagatgcggc tctccgtgtc ttgattgggg agaacgagaa
     3301 ggcagaacgc atgggtttca cagaggtaac cccagtgaca ggggccagtc tcagaagaac
     3361 tatgctcctc ctctcaaggt ccccagaagc acagccaaag acactccctc tcactggcag
     3421 caccttccat gaccagatag ccatgctgag ccaccggtgc ttcaacactc tgactaacag
     3481 cttccagccc tccttgctcg gccgcaagat tctggccgcc atcattatga aaaaagactc
     3541 tgaggacatg ggtgtcgtcg tcagcttggg aacagggaat cgctgtgtaa aaggagattc
     3601 tctcagccta aaaggagaaa ctgtcaatga ctgccatgca gaaataatct cccggagagg
     3661 cttcatcagg tttctctaca gtgagttaat gaaatacaac tcccagactg cgaaggatag
     3721 tatatttgaa cctgctaagg gaggagaaaa gctccaaata aaaaagactg tgtcattcca
     3781 tctgtatatc agcactgctc cgtgtggaga tggcgccctc tttgacaagt cctgcagcga
     3841 ccgtgctatg gaaagcacag aatcccgcca ctaccctgtc ttcgagaatc ccaaacaagg
     3901 aaagctccgc accaaggtgg agaacggaga aggcacaatc cctgtggaat ccagtgacat
     3961 tgtgcctacg tgggatggca ttcggctcgg ggagagactc cgtaccatgt cctgtagtga
     4021 caaaatccta cgctggaacg tgctgggcct gcaaggggca ctgttgaccc acttcctgca
     4081 gcccatttat ctcaaatctg tcacattggg ttaccttttc agccaagggc atctgacccg
     4141 tgctatttgc tgtcgtgtga caagagatgg gagtgcattt gaggatggac tacgacatcc
     4201 ctttattgtc aaccacccca aggttggcag agtcagcata tatgattcca aaaggcaatc
     4261 cgggaagact aaggagacaa gcgtcaactg gtgtctggct gatggctatg acctggagat
     4321 cctggacggt accagaggca ctgtggatgg gccacggaat gaattgtccc gggtctccaa
     4381 aaagaacatt tttcttctat ttaagaagct ctgctccttc cgttaccgca gggatctact
     4441 gagactctcc tatggtgagg ccaagaaagc tgcccgtgac tacgagacgg ccaagaacta
     4501 cttcaaaaaa ggcctgaagg atatgggcta tgggaactgg attagcaaac cccaggagga
     4561 aaagaacttt tatctctgcc cagtagcggc cgcacatcac catcatcacc attgactcga
     4621 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4681 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4741 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4801 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4861 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4921 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4981 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5041 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5101 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5161 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5221 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5281 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5341 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5401 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5521 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5581 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     5641 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5701 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     5761 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5821 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5881 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5941 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6001 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6061 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6121 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6181 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6241 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6301 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6361 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6421 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6481 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6541 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6601 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6661 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6721 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6781 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6841 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     6901 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6961 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7021 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7081 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7141 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7201 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7261 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7501 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7741 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     7861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7981 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8041 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8101 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8161 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8221 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8281 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8341 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8401 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8461 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8521 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8581 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8641 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8701 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8761 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8821 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8881 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8941 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9001 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9061 acggatcggg agatctcccg atcccctatg gt
//
