LOCUS V029515 9804 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029515 VERSION V029515 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 1960..2171 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 2184..2452 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 2486..4675 /label="DTL(NM_016448)" /note="DTL(NM_016448)" /gene="DTL" CDS 4682..4747 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 4773..5272 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5293..5892 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5899..6487 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6561..6794 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6866..6987 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7006..7141 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7179..7195) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7203..7219 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7227..7257) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7272..7293 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7581..8169) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8340..9200) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9201..9305) /gene="bla" /label="AmpR promoter" primer_bind 9779..9795 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ccaccatgct cttcaattcg gtgctccgcc agccccagct 2521 tggcgtcctg agaaatggat ggtcttcaca ataccctctt caatcccttc tgactggtta 2581 tcagtgcagt ggtaatgatg aacacacttc ttatggagaa acaggagtcc cagttcctcc 2641 ttttggatgt accttctctt ctgctcccaa tatggaacat gtactagcag ttgccaatga 2701 agaaggcttt gttcgattgt ataacacaga atcacaaagt ttcagaaaga agtgcttcaa 2761 agaatggatg gctcactgga atgccgtctt tgacctggcc tgggttcctg gtgaacttaa 2821 acttgttaca gcagcaggtg atcaaacagc caaattttgg gacgtaaaag ctggtgagct 2881 gattggaaca tgcaaaggtc atcaatgcag cctcaagtca gttgcctttt ctaagtttga 2941 gaaagctgta ttctgtacgg gtggaagaga tggcaacatt atggtctggg ataccaggtg 3001 caacaaaaaa gatgggtttt ataggcaagt gaatcaaatc agtggagctc acaatacctc 3061 agacaagcaa accccttcaa aacccaagaa gaaacagaat tcaaaaggac ttgctccttc 3121 tgtggatttc cagcaaagtg ttactgtggt cctctttcaa gacgagaata ccttagtctc 3181 agcaggagct gtggatggga taatcaaagt atgggattta cgtaagaatt atactgctta 3241 tcgacaagaa cccatagcat ccaagtcttt cctgtaccca ggtagcagca ctcgaaaact 3301 tggatattca agtctgattt tggattccac tggctctact ttatttgcta attgcacaga 3361 cgataacatc tacatgttta atatgactgg gttgaagact tctccagtgg ctattttcaa 3421 tggacaccag aactctacct tttatgtaaa atccagcctt agtccagatg accagttttt 3481 agtcagtggc tcaagtgatg aagctgccta catatggaag gtctccacac cctggcaacc 3541 tcctactgtg ctcctgggtc attctcaaga ggtcacgtct gtgtgctggt gtccatctga 3601 cttcacaaag attgctacct gttctgatga caatacacta aaaatctggc gcttgaatag 3661 aggcttagag gagaaaccag gaggtgataa actttccacg gtgggttggg cctctcagaa 3721 gaaaaaagag tcaagacctg gcctagtaac agtaacgagt agccagagta ctcctgccaa 3781 agcccccagg gcaaagtgca atccatccaa ttcttccccg tcatccgcag cttgtgcccc 3841 aagctgtgct ggagacctcc ctcttccttc aaatactcct acgttctcta ttaaaacctc 3901 tcctgccaag gcccggtctc ccatcaacag aagaggctct gtctcctccg tctctcccaa 3961 gccaccttca tctttcaaga tgtcgattag aaactgggtg acccgaacac cttcctcatc 4021 accacccatc actccacctg cttcggagac caagatcatg tctccgagaa aagcccttat 4081 tcctgtgagc cagaagtcat cccaagcaga ggcttgctct gagtctagaa atagagtaaa 4141 gaggaggcta gactcaagct gtctggagag tgtgaaacaa aagtgtgtga agagttgtaa 4201 ctgtgtgact gagcttgatg gccaagttga aaatcttcat ttggatctgt gctgccttgc 4261 tggtaaccag gaagacctta gtaaggactc tctaggtcct accaaatcaa gcaaaattga 4321 aggagctggt accagtatct cagagcctcc gtctcctatc agtccgtatg cttcagaaag 4381 ctgtggaacg ctacctcttc ctttgagacc ttgtggagaa gggtctgaaa tggtaggcaa 4441 agagaatagt tccccagaga ataaaaactg gttgttggcc atggcagcca aacggaaggc 4501 tgagaatcca tctccacgaa gtccgtcatc ccagacaccc aattccagga gacagagcgg 4561 aaagaaattg ccaagcccgg tcaccatcac gcccagctcc atgaggaaaa tctgcacata 4621 cttccataga aagtcccagg aggacttctg tggtcctgaa cactcaacag aattagctag 4681 cgactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga 4741 tgacaagtga attcgaagga tccgcggccg ctgggtaggg gaggcgcttt tcccaaggca 4801 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg 4861 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc 4921 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc 4981 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag 5041 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc 5101 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg 5161 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca 5221 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag 5281 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5341 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5401 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5461 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 5521 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 5581 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 5641 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 5701 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 5761 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 5821 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5881 cccggtgcct gagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 6301 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 6481 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6541 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 6601 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6661 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6721 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6781 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6841 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6901 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6961 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 7021 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 7081 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 7141 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 7201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 7261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 7321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 7381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 7441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 7501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 8041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 8101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 8821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 8881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 8941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 9001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 9061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 9121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 9181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 9241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 9301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 9361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 9421 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 9481 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 9541 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 9601 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 9661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 9721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 9781 aaaacgacgg ccagtgccaa gctg //