LOCUS       V013932                 9108 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013932
VERSION     V013932
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2294..3256
                     /gene="mraY"
                     /label="Phospho-N-acetylmuramoyl-pentapeptide-transferase"
                     /note="Phospho-N-acetylmuramoyl-pentapeptide-transferase
                     from Staphylococcus aureus (strain JH9). Accession#:
                     A5IS68"
     CDS             3257..3280
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     promoter        3329..3540
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             3553..3821
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3856..4548
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     CDS             4603..5199
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5203..5791
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5865..6098
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6170..6304
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6310..6445
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6483..6499)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6507..6523)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6531..6561)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6576..6597)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6885..7473)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7647..8504)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8505..8609)
                     /label="AmpR promoter"
     primer_bind     9083..9099
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatgattt ttgtatatgc gttattagcg ctagtgatta catttgtttt
     2341 ggtacctgtt ttaataccta cattaaaaag gatgaaattt ggtcaaagta ttcgagaaga
     2401 aggtccacaa agccatatga agaagactgg tacaccaacg atgggtggac taacatttct
     2461 attaagtatt gtgataacgt ctttggtggc tattatattt gtagatcaag ctaatccaat
     2521 catactgtta ttatttgtga cgattggttt tgggttaatt ggttttatag atgattatat
     2581 tattgttgtt aaaaagaata accaaggttt aacaagtaaa cagaagtttt tggcgcaaat
     2641 tggtattgcg attatattct ttgttttaag taatgtgttt catttggtga atttttctac
     2701 gagcatacat attccattta cgaatgtagc aatcccacta tcatttgcat atgttatttt
     2761 cattgttttt tggcaagtag gtttttctaa tgcagtaaat ttaacagatg gtttagatgg
     2821 attagcaact ggactgtcaa ttatcggatt tacaatgtat gccatcatga gctttgtgtt
     2881 aggagaaacg gcaattggta ttttctgtat cattatgttg tttgcacttt taggattttt
     2941 accatataac attaaccctg ctaaagtgtt tatgggagat acaggtagct tagctttagg
     3001 tggtatattt gctaccattt caatcatgct taatcaggaa ttatcattaa tttttatagg
     3061 tttagtattc gtaattgaaa cattatctgt tatgttacaa gtcgctagct ttaaattgac
     3121 tggaaagcgt atatttaaaa tgagtccgat tcatcatcat tttgaattga taggatggag
     3181 cgaatggaaa gtagttacag tattttgggc tgttggtctg atttcaggtt taatcggttt
     3241 atggattgga gtgcatgatt acaaggacga cgatgacaag taggatccgc ggccgcaagg
     3301 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3361 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     3421 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3481 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3541 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     3601 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     3661 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     3721 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     3781 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     3841 acgctagacg ccaccatgag cgagctgatc aaggagaaca tgcacatgaa gctgtacatg
     3901 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     3961 gagggcaccc agaccatgaa gatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     4021 gacatcctgg ctaccagctt catgtacggc agcaaagcct tcatcaacca cacccagggc
     4081 atccccgact tctttaagca gtccttccct gagggcttca catgggagag aatcaccaca
     4141 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcttccagaa cggctgcatc
     4201 atctacaacg tcaagatcaa cggggtgaac ttcccatcca acggccctgt gatgcagaag
     4261 aaaacacgcg gctgggaggc caacaccgag atgctgtacc ccgctgacgg cggcctgaga
     4321 ggccacagcc agatggccct gaagctcgtg ggcgggggct acctgcactg ctccttcaag
     4381 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggctt ccacttcgtg
     4441 gaccacagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     4501 atggctgtgg ccaagtactg cgacctccct agcaaactgg ggcacagaga gggcagagga
     4561 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     4621 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4681 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4741 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4801 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4861 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4921 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4981 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5041 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5101 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5161 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     5221 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5281 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5341 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5401 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5461 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5521 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5581 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5641 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5701 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5761 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     5821 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     5881 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5941 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6001 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6061 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6121 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6301 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6361 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6421 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     6481 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7321 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8641 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8701 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8761 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8821 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8881 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8941 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     9001 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     9061 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
