LOCUS       V022556                10922 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022556
VERSION     V022556
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5064
                     /label="Dpp9(NM_001360284)"
                     /note="Dpp9(NM_001360284)"
                     /gene="Dpp9"
     CDS             5071..5100
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     promoter        5143..5354
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5367..5635
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5670..6362
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             6363..6416
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6417..7016
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7017..7605
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7679..7912
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7984..8105
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8124..8259
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8297..8313)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8321..8337
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8345..8375)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8390..8411
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8699..9287)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9458..10318)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10319..10423)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10897..10913
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgttggca gccccgaggg gctgggaggc tcagttagag
     2341 tcgaaccagg tagatagggt taggcacagg cggagactcc cgcccgcatg gaaagtccgt
     2401 gcccctcgcc tgcagctgcc atggaagctc cttggaccct ttagaagctt ctcgccgcca
     2461 tcacaggagc cccagaggat gtgcagcggg gtctccccag ttgagcaggt ggccgcaggg
     2521 gacatggatg acacggcagc acgcttctgt gtgcagaagc actcgtggga tgggctgcgt
     2581 agcattatcc acggcagtcg caagtcctcg ggcctcattg tcagcaaggc cccccacgac
     2641 ttccagtttg tgcagaagcc tgacgagtct ggcccccact ctcaccgtct ctattacctc
     2701 ggaatgcctt acggcagccg tgagaactcc ctcctctact ccgagatccc caagaaagtg
     2761 cggaaggagg ccctgctgct gctgtcctgg aagcagatgc tggaccactt ccaggccaca
     2821 ccccaccatg gtgtctactc ccgagaggag gagctactgc gggagcgcaa gcgcctgggc
     2881 gtcttcggaa tcacctctta tgacttccac agtgagagcg gcctcttcct cttccaggcc
     2941 agcaatagcc tgttccactg cagggatggt ggcaagaatg gctttatggt gtccccgatg
     3001 aagccactgg agatcaagac tcagtgttct gggccacgca tggaccccaa aatctgcccc
     3061 gcagaccctg ccttcttttc cttcatcaac aacagtgatc tgtgggtggc aaacatcgag
     3121 actggggagg aacggcggct caccttctgt caccagggtt cagctggtgt cctggacaat
     3181 cccaaatcag caggcgtggc cacctttgtc atccaggagg agttcgaccg cttcactggg
     3241 tgctggtggt gccccacggc ctcttgggaa ggctccgaag gtctcaagac gctgcgcatc
     3301 ctatatgagg aagtggacga gtctgaagtg gaggtcattc atgtgccctc ccccgccctg
     3361 gaggagagga agacggactc ctaccgctac cccaggacag gcagcaagaa ccccaagatt
     3421 gccctgaagc tggctgagct ccagacggac catcagggca aaatcgtgtc aagctgcgag
     3481 aaggaactgg tacagccatt cagctccctt ttccccaaag tggagtacat cgcccgggct
     3541 ggctggacac gggacggcaa atatgcctgg gccatgttcc tggaccgtcc ccagcaacgg
     3601 cttcagcttg tcctcctgcc ccctgctctc ttcatcccgg ccgttgagag tgaggcccag
     3661 cggcaggcag ctgccagagc cgtccccaag aatgtgcagc cctttgtcat ctatgaagaa
     3721 gtcaccaatg tctggatcaa cgtccacgac atcttccacc cgtttcctca ggctgagggc
     3781 cagcaggact tttgtttcct tcgtgccaac gaatgcaaga ctggcttctg ccacctgtac
     3841 agggtcacag tggaacttaa aaccaaggac tatgactgga cggaacccct cagccctaca
     3901 gaagatgagt ttaagtgccc catcaaggag gaggtcgccc tgaccagtgg cgagtgggag
     3961 gtcttgtcga ggcatggctc caagatctgg gtcaacgagc agacgaagct ggtgtacttt
     4021 caaggtacaa aggacacacc gctggaacat cacctctatg tggtcagcta cgagtcagca
     4081 ggcgagatcg tgcggctcac cacgctcggc ttctcccaca gctgctccat gagccagagc
     4141 ttcgacatgt tcgtgagtca ctacagcagt gtgagcacgc caccctgtgt acatgtgtac
     4201 aagctgagcg gccccgatga tgacccactg cacaagcaac cacgcttctg ggccagcatg
     4261 atggaggcag ccaattgccc cccagactat gtgccccctg agatcttcca cttccacacc
     4321 cgtgcagacg tgcagctcta cggcatgatc tacaagccac acaccctgca acctgggagg
     4381 aagcacccca ctgtgctctt tgtctatggg ggcccacagg tgcagttggt gaacaactcc
     4441 tttaagggca tcaaatacct gcggctaaat acactggcat ccttgggcta tgctgtggtg
     4501 gtgatcgatg gtcggggctc ctgtcagcgg ggcctgcact tcgagggggc cctgaaaaat
     4561 caaatgggcc aggtggagat tgaggaccag gtggaaggct tgcagtacgt ggctgagaag
     4621 tatggcttca ttgacttgag ccgagtcgcc atccatggct ggtcctacgg cggcttcctc
     4681 tcactcatgg ggctcatcca caagccacaa gtgttcaagg tagccattgc gggcgctcct
     4741 gtcactgtgt ggatggccta tgacacaggg tacacggaac gatacatgga tgtccccgaa
     4801 aataaccagc aaggctatga ggcagggtct gtagccctgc atgtggagaa gctgcccaat
     4861 gagcctaacc gcctgcttat cctccacggc ttcctggacg agaacgttca cttcttccac
     4921 acaaatttcc tggtgtccca gctgatccga gcaggaaagc cataccagct tcagatctac
     4981 ccaaacgaga gacatagcat ccgctgccgc gagtccggag agcattacga ggtgacgctg
     5041 ctgcactttc tgcaggaaca cctgggatcc gagcagaagc tgatctcaga ggaggacctg
     5101 tagcggccgc aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc
     5161 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     5221 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     5281 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     5341 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     5401 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     5461 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     5521 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     5581 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     5641 tgtgaccggc gcctacgcta gacgccacca tgagcgagct gatcaaggag aacatgcaca
     5701 tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg
     5761 aaggcaagcc ctacgagggc acccagacca tgaagatcaa ggtggtcgag ggcggccctc
     5821 tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa gccttcatca
     5881 accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg
     5941 agagaatcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcttcc
     6001 agaacggctg catcatctac aacgtcaaga tcaacggggt gaacttccca tccaacggcc
     6061 ctgtgatgca gaagaaaaca cgcggctggg aggccaacac cgagatgctg taccccgctg
     6121 acggcggcct gagaggccac agccagatgg ccctgaagct cgtgggcggg ggctacctgc
     6181 actgctcctt caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg
     6241 gcttccactt cgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg
     6301 tcgagcagca cgagatggct gtggccaagt actgcgacct ccctagcaaa ctggggcaca
     6361 gagagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga
     6421 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6481 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6541 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6601 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6661 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6721 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6781 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6841 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6901 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6961 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc
     7021 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7081 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7141 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7201 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7261 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7381 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7441 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7501 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7561 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     7621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7681 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc
     7741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7921 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7981 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8041 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8101 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc
     8161 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8221 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc
     8281 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8341 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8401 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8461 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9121 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10441 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10501 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10561 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10621 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10681 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10741 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10801 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10861 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
    10921 tg
//
