LOCUS V043239 11664 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043239 VERSION V043239 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..5835 /label="Adar(NM_001146296)" /note="Adar(NM_001146296)" /gene="Adar" promoter 5885..6096 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 6109..6377 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 6412..7104 /codon_start=1 /note="mammalian codon-optimized" /product="red fluorescent protein from Entacmaea quadricolor" /transl_table=1 /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC DLPSKLGHR" /label="TurboRFP" CDS 7105..7158 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 7159..7758 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 7759..8347 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 8421..8654 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 8726..8847 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 8866..9001 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(9039..9055) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 9063..9079 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(9087..9117) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 9132..9153 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(9441..10029) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(10200..11060) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(11061..11165) /gene="bla" /label="AmpR promoter" primer_bind 11639..11655 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catgtctcaa gggttcaggg gacccacagg ggtgttccct 2341 caccagacac agtcgtactt ggaccctagt catgagcata gcaagtggag atacccgcag 2401 ccacaggggc cggagtctta ccctaggagt ttccagcttc agcagataga gtttctcaaa 2461 gggcggctcc cagaagcacc cttgattgga atacaaaccc agtcactgcc gccattcctc 2521 ccaggacact ggccaagatt cccagggcca cctgcccaag acaggcaact ggaaatctgg 2581 gagttcccca ggagtgtgac tctcagaaat caggggttcc acataggacc cccacttcct 2641 cccccacaca gcaggggtac accatggaga ggtgctgacg ggctttgctc acacttccgg 2701 gagctgagca tcagtcagag tccggagcag aaggttctaa accgcctgga agagcttggg 2761 gaggggaagg ccaccactgc ccatgtgcta gccagagagc tcagaatccc caaaagggac 2821 atcaatcgta ttttgtactc cctggaaaag aagggaaagc tgcacagagg aagggggaaa 2881 cctcctttgt ggagccttgt gcccttgagt caggcttgga ctcagccccc tggagttgtg 2941 aatccagata gttgtatcca ggaattccct agaggagagc ctggtttgga cagtgaggac 3001 ggagaccctg cctctgactt agaaggacct tctgagcctc ttgacatggc tgaaatcaag 3061 gagaagatct gtgactatct gttcaatgtg tcaaactcct ctgccctgaa cctggctaag 3121 aacattggcc tcaccaaggc ccgagatgtg acctcagtgc tgattgactt ggaaaggcaa 3181 ggcgatgtct acaggcaagg ggcaactcct cccatctggt acttgacgga caagaagcgt 3241 gagaggctgc agatgaagag aagtacacac agtgctcctg cccctacccc gacagctgtc 3301 ccagaggcca ctagaagccc ctcattccct gcctgccacc cgcccccagc aggtgcctca 3361 agcagtgtgg cagcctccaa gagagtggag aatgggcagg agcctgcgat aaagcatgaa 3421 agtaggcatg aggccagacc aggaccaatg agactgcggc ctcacgctta tcacaatggc 3481 ccctctagag cagggtatgt ggcctctgaa aatggccagt gggccacaga tgacatccca 3541 gataacttga atagtatcca cacagcacca ggtgagtttc gagccatcat ggagatgccc 3601 tccttctaca gccctacctt gccacggtgt tcaccctaca agaagctaac tgagtgccag 3661 ctgaagaacc ctgtcagcgg gttgttagag tatgctcagt tcactagtca gacctgtgat 3721 ttcaacctga tagagcagag tggaccgtcc catgaacctc gatttaaatt ccaggttgtc 3781 atcaatgggc gggaatttcc cccagctgag gctggcagca agaaagtagc caagcaggac 3841 gcagcagtga aagccatggc gattctgctt cgggaagcca aagccaaaga cagtggtcaa 3901 ccagaagact tgtcccactg tcccatggaa gaagactcgg agaaaccagc agaggctcag 3961 gcccccagct cctcagcaac atccttgttc tctgggaaga gcccagttac tacactgctt 4021 gagtgcatgc acaaactagg gaactcctgt gaattccgtc tcctgtccaa agaaggccct 4081 gctcatgacc ccaagttcca gtactgtgta gcagtaggag cccagacctt cccccctgtg 4141 agcgccccca gcaagaaggt agcaaagcag atggccgcag aggaagccat gaaggcgctg 4201 caggaggagg cagccagttc agcggatgac cagtctggag gtgcgaacac agactcactt 4261 gatgaatcta tggctcccaa caagatcagg aggattggtg agctcgtcag gtacctgaac 4321 accaaccccg taggcggctt gttggagtac gcccgatctc atggctttgc tgctgagttc 4381 aagctcattg accagtctgg acctcctcac gaacccaagt ttgtttacca agcaaaagtt 4441 gggggccgct ggtttccagc cgtgtgtgca cacagcaaga aacagggcaa gcaggatgca 4501 gcggatgcag ccctccgggt cttgatcggg gagagcgaga aggcagagca gttgggtttc 4561 gcagaggtaa ccccagtaac aggggccagt ctcagaagaa ctatgctcct cctttccagg 4621 tccccagatg cacatccaaa gacacttcct ctctctggca gcaccttcca cgaccagata 4681 gctatgctga gccacaggtg cttcaatgct ctgaccaaca gtttccagcc ctccctgctc 4741 ggccgcaaga tcctggctgc cattattatg aaaagagatc cagaggacat gggtgttgtc 4801 gtgagtttgg ggacaggcaa tcgctgtgtg aaaggggact ctctcagcct gaagggagag 4861 acggtcaatg actgccatgc cgaaatcatc tcccggaggg gcttcatcag gtttctctac 4921 agtgaactga tgaagtacaa ccaccacact gccaagaaca gcatatttga gcttgccagg 4981 ggaggagaga agctgcagat aaagaagacg gtttcttttc atctctacat cagcacggcg 5041 ccatgtggag atggggccct ctttgacaaa tcctgcagtg accgtgccgt ggaaagcaca 5101 gagtcccgcc attaccctgt ctttgaaaat cccaagcaag gcaagcttcg caccaaggtg 5161 gagaatgggg aaggcacaat tcctgtggag tccagtgata ttgtacccac gtgggatggc 5221 atccgccttg gggaaagact ccgtaccatg tcctgtagtg acaaaatcct acgctggaat 5281 gtgctgggcc tgcaaggggc gttgttgacg cacttcctac agcctgtgta cctgaaatct 5341 gtaaccttag gttacctttt cagccaaggg catctgaccc gtgctatttg ctgccgcgtg 5401 accagagatg ggaaagcatt tgaggatgga ctacgctatc cctttattgt caaccacccc 5461 aaggtcggcc gagtcagtgt ttatgattcc aaaagacagt ccggaaagac caaggaaaca 5521 agcgtcaact ggtgcatggc tgatggctat gacctagaga tcctggatgg caccagaggc 5581 actgtggatg gaccagggaa agagttgtct cgggtgtcca agaagaatat tttccttcag 5641 tttaagaagc tctgctcctt ccgagcccgc agagatttac tgcagctctc ttatggtgaa 5701 gccaagaaag ctgcccgtga ctatgactta gccaagaact acttcaagaa aagcctgcga 5761 gacatgggct atgggaattg gatcagcaaa ccccaggagg aaaagaactt ttacctctgt 5821 ccagtaccca atgactgagg atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt 5881 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 5941 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 6001 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 6061 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc 6121 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt 6181 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag 6241 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg 6301 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt 6361 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catgagcgag 6421 ctgatcaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac 6481 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc 6541 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg 6601 tacggcagca aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc 6661 ttccctgagg gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc 6721 gctacccagg acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg 6781 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac 6841 accgagatgc tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag 6901 ctcgtgggcg ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc 6961 gctaagaacc tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag 7021 gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac 7081 ctccctagca aactggggca cagagagggc agaggaagtc ttctaacatg cggtgacgtg 7141 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 7201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7741 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 7801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 7861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 7921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 7981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 8041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 8101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 8161 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 8221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 8281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 8341 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 8461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 8521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8641 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 8701 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 8761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8821 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 8881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 8941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 9001 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 9061 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 9121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 9181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 9241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 9301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 9361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 9421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 9481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 9541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 9601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 9661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 9721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 9781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 9841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 9901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 9961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 10021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 10081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 10141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 10201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 10261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 10321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 10381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 10441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 10501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 10561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 10621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 10681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 10741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 10801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 10861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 10921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 10981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 11041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 11101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 11161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 11221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 11281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 11341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 11401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 11461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 11521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 11581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 11641 aaaacgacgg ccagtgccaa gctg //