LOCUS       pCDF-1b.        3621 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector with a CloDF13 (CDF) origin for the regulated 
            expression of N-terminally 6xHis-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pCDF-1b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..73
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             77..94
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             113..127
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             206..250
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     terminator      302..349
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(523..1311)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(1312..1403)
                     /label=AmpR promoter
     rep_origin      complement(1451..2189)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(2365..2386)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2402..3481)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3482..3559)
                     /label=lacI promoter
     promoter        3605..3621
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggcacatc accaccacca tcacgtgggt accggttcga atgatgacga
      121 cgacaagagt ccggatccca attgggagct cgtgtacacg gcgcgcctgc aggtcgacaa
      181 gcttgcggcc gcactcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca
      241 catggactcg tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata
      301 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca
      361 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
      421 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc
      481 ggatcttgcg gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
      541 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
      601 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
      661 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
      721 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
      781 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
      841 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
      901 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
      961 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     1021 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     1081 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     1141 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     1201 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     1261 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct
     1321 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1381 atgtatttag aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag
     1441 actgcggcgg gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg
     1501 gactaacatg tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc
     1561 cctcctgcca gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg
     1621 ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt
     1681 tccggcgggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     1741 gttccgcctt tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc
     1801 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
     1861 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta
     1921 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     1981 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     2041 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     2101 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     2161 aaaaaggatc tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc
     2221 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct
     2281 catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2341 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     2401 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2461 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     2521 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     2581 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     2641 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc
     2701 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     2761 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     2821 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     2881 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     2941 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     3001 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     3061 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     3121 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     3181 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     3241 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     3301 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     3361 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     3421 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     3481 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     3541 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     3601 aaattaatac gactcactat a
//
