LOCUS       .                       4864 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        67..98
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             134..1783
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(1827..1948)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2367..2955)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3129..3986)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3987..4091)
                     /label="AmpR promoter"
     rep_origin      4118..4573
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4704..4752
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4766..4857
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human α2 globin gene"
ORIGIN
        1 ggtaccgggg ttttccgggg ttttccgggg ttttccgggg ttttccgggg ttttccgggg
       61 ttttcctaga gggtatataa tggaagctcg acttccagaa gcttggcatt ccggtactgt
      121 tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
      181 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
      241 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
      301 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
      361 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
      421 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
      481 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
      541 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
      601 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
      661 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
      721 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
      781 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
      841 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
      901 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
      961 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     1021 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     1081 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     1141 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     1201 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     1261 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     1321 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     1381 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     1441 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     1501 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
     1561 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     1621 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     1681 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     1741 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg
     1801 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     1861 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     1921 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1981 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     2041 aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     2101 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     2161 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3001 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3061 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3121 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3181 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3241 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3301 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3361 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3421 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3481 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3541 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3601 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3661 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3721 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3781 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3841 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3901 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3961 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4081 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     4141 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4201 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4261 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4321 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4381 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4441 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4501 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4561 acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4621 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta
     4681 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     4741 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa
     4801 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc
     4861 gata
//
