LOCUS       40924_9246        8139 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCC1FOS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   EPICENTRE Biotechnologies.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA
REFERENCE   2  (bases 1 to 8139)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     288..304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        311..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(443..459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(467..483)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(491..521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(536..557)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(780..1436)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1437..1539)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2397..3011
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      3087..3306
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             3397..4149
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    4155..4405
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             4731..5903
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5906..6874
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    6950..7423
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(7683..8081)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(8099..8132)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatccca
      361 cgtgggatcc tctagagtcg acctgcaggc atgcaagctt gagtattcta tagtctcacc
      421 taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      481 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      541 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      601 cgtgccagct gcattaatga atcggccaac gcgaacccct tgcggccgcc cgggccgtcg
      661 accaattctc atgtttgaca gcttatcatc gaatttctgc cattcatccg cttattatca
      721 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
      781 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      841 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      901 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      961 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     1021 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1081 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1141 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1201 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1261 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1321 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1381 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1441 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1501 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
     1561 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     1621 tcttccgtca caggtattta ttcgcgataa gctcatggag cggcgtaacc gtcgcacagg
     1681 aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga cctggattgg
     1741 ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg tcacaccaca
     1801 tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc tgaaagttct
     1861 gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga aggtttttgc
     1921 gctggatgtg gctgcccggc accgggtgca gtttgcgatg ccggagtctg atgcggttgc
     1981 gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa atgtggaact
     2041 gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc actgagaagc
     2101 gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt aatctcagga
     2161 gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc ggtaacgaaa
     2221 acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac gttgaagtgg
     2281 agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat gatgtggtct
     2341 gtccttttac agccagtagt gctcgccgca gtcgagcgac agggcgaagc cctcggctgg
     2401 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     2461 cgaagccgtg tgcgagacac cgcggccggc cgccggcgtt gtggatacct cgcggaaaac
     2521 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     2581 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     2641 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     2701 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     2761 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     2821 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     2881 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     2941 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     3001 cgaaccctcc cggtcgagtg agcgaggaag caccagggaa cagcacttat atattctgct
     3061 tacacacgat gcctgaaaaa acttcccttg gggttatcca cttatccacg gggatatttt
     3121 tataattatt ttttttatag tttttagatc ttctttttta gagcgccttg taggccttta
     3181 tccatgctgg ttctagagaa ggtgttgtga caaattgccc tttcagtgtg acaaatcacc
     3241 ctcaaatgac agtcctgtct gtgacaaatt gcccttaacc ctgtgacaaa ttgccctcag
     3301 aagaagctgt tttttcacaa agttatccct gcttattgac tcttttttat ttagtgtgac
     3361 aatctaaaaa cttgtcacac ttcacatgga tctgtcatgg cggaaacagc ggttatcaat
     3421 cacaagaaac gtaaaaatag cccgcgaatc gtccagtcaa acgacctcac tgaggcggca
     3481 tatagtctct cccgggatca aaaacgtatg ctgtatctgt tcgttgacca gatcagaaaa
     3541 tctgatggca ccctacagga acatgacggt atctgcgaga tccatgttgc taaatatgct
     3601 gaaatattcg gattgacctc tgcggaagcc agtaaggata tacggcaggc attgaagagt
     3661 ttcgcgggga aggaagtggt tttttatcgc cctgaagagg atgccggcga tgaaaaaggc
     3721 tatgaatctt ttccttggtt tatcaaacgt gcgcacagtc catccagagg gctttacagt
     3781 gtacatatca acccatatct cattcccttc tttatcgggt tacagaaccg gtttacgcag
     3841 tttcggctta gtgaaacaaa agaaatcacc aatccgtatg ccatgcgttt atacgaatcc
     3901 ctgtgtcagt atcgtaagcc ggatggctca ggcatcgtct ctctgaaaat cgactggatc
     3961 atagagcgtt accagctgcc tcaaagttac cagcgtatgc ctgacttccg ccgccgcttc
     4021 ctgcaggtct gtgttaatga gatcaacagc agaactccaa tgcgcctctc atacattgag
     4081 aaaaagaaag gccgccagac gactcatatc gtattttcct tccgcgatat cacttccatg
     4141 acgacaggat agtctgaggg ttatctgtca cagatttgag ggtggttcgt cacatttgtt
     4201 ctgacctact gagggtaatt tgtcacagtt ttgctgtttc cttcagcctg catggatttt
     4261 ctcatacttt ttgaactgta atttttaagg aagccaaatt tgagggcagt ttgtcacagt
     4321 tgatttcctt ctctttccct tcgtcatgtg acctgatatc gggggttagt tcgtcatcat
     4381 tgatgagggt tgattatcac agtttattac tctgaattgg ctatccgcgt gtgtacctct
     4441 acctggagtt tttcccacgg tggatatttc ttcttgcgct gagcgtaaga gctatctgac
     4501 agaacagttc ttctttgctt cctcgccagt tcgctcgcta tgctcggtta cacggctgcg
     4561 gcgagcgcta gtgataataa gtgactgagg tatgtgctct tcttatctcc ttttgtagtg
     4621 ttgctcttat tttaaacaac tttgcggttt tttgatgact ttgcgatttt gttgttgctt
     4681 tgcagtaaat tgcaagattt aataaaaaaa cgcaaagcaa tgattaaagg atgttcagaa
     4741 tgaaactcat ggaaacactt aaccagtgca taaacgctgg tcatgaaatg acgaaggcta
     4801 tcgccattgc acagtttaat gatgacagcc cggaagcgag gaaaataacc cggcgctgga
     4861 gaataggtga agcagcggat ttagttgggg tttcttctca ggctatcaga gatgccgaga
     4921 aagcagggcg actaccgcac ccggatatgg aaattcgagg acgggttgag caacgtgttg
     4981 gttatacaat tgaacaaatt aatcatatgc gtgatgtgtt tggtacgcga ttgcgacgtg
     5041 ctgaagacgt atttccaccg gtgatcgggg ttgctgccca taaaggtggc gtttacaaaa
     5101 cctcagtttc tgttcatctt gctcaggatc tggctctgaa ggggctacgt gttttgctcg
     5161 tggaaggtaa cgacccccag ggaacagcct caatgtatca cggatgggta ccagatcttc
     5221 atattcatgc agaagacact ctcctgcctt tctatcttgg ggaaaaggac gatgtcactt
     5281 atgcaataaa gcccacttgc tggccggggc ttgacattat tccttcctgt ctggctctgc
     5341 accgtattga aactgagtta atgggcaaat ttgatgaagg taaactgccc accgatccac
     5401 acctgatgct ccgactggcc attgaaactg ttgctcatga ctatgatgtc atagttattg
     5461 acagcgcgcc taacctgggt atcggcacga ttaatgtcgt atgtgctgct gatgtgctga
     5521 ttgttcccac gcctgctgag ttgtttgact acacctccgc actgcagttt ttcgatatgc
     5581 ttcgtgatct gctcaagaac gttgatctta aagggttcga gcctgatgta cgtattttgc
     5641 ttaccaaata cagcaatagt aatggctctc agtccccgtg gatggaggag caaattcggg
     5701 atgcctgggg aagcatggtt ctaaaaaatg ttgtacgtga aacggatgaa gttggtaaag
     5761 gtcagatccg gatgagaact gtttttgaac aggccattga tcaacgctct tcaactggtg
     5821 cctggagaaa tgctctttct atttgggaac ctgtctgcaa tgaaattttc gatcgtctga
     5881 ttaaaccacg ctgggagatt agataatgaa gcgtgcgcct gttattccaa aacatacgct
     5941 caatactcaa ccggttgaag atacttcgtt atcgacacca gctgccccga tggtggattc
     6001 gttaattgcg cgcgtaggag taatggctcg cggtaatgcc attactttgc ctgtatgtgg
     6061 tcgggatgtg aagtttactc ttgaagtgct ccggggtgat agtgttgaga agacctctcg
     6121 ggtatggtca ggtaatgaac gtgaccagga gctgcttact gaggacgcac tggatgatct
     6181 catcccttct tttctactga ctggtcaaca gacaccggcg ttcggtcgaa gagtatctgg
     6241 tgtcatagaa attgccgatg ggagtcgccg tcgtaaagct gctgcactta ccgaaagtga
     6301 ttatcgtgtt ctggttggcg agctggatga tgagcagatg gctgcattat ccagattggg
     6361 taacgattat cgcccaacaa gtgcttatga acgtggtcag cgttatgcaa gccgattgca
     6421 gaatgaattt gctggaaata tttctgcgct ggctgatgcg gaaaatattt cacgtaagat
     6481 tattacccgc tgtatcaaca ccgccaaatt gcctaaatca gttgttgctc ttttttctca
     6541 ccccggtgaa ctatctgccc ggtcaggtga tgcacttcaa aaagccttta cagataaaga
     6601 ggaattactt aagcagcagg catctaacct tcatgagcag aaaaaagctg gggtgatatt
     6661 tgaagctgaa gaagttatca ctcttttaac ttctgtgctt aaaacgtcat ctgcatcaag
     6721 aactagttta agctcacgac atcagtttgc tcctggagcg acagtattgt ataagggcga
     6781 taaaatggtg cttaacctgg acaggtctcg tgttccaact gagtgtatag agaaaattga
     6841 ggccattctt aaggaacttg aaaagccagc accctgatgc gaccacgttt tagtctacgt
     6901 ttatctgtct ttacttaatg tcctttgtta caggccagaa agcataactg gcctgaatat
     6961 tctctctggg cccactgttc cacttgtatc gtcggtctga taatcagact gggaccacgg
     7021 tcccactcgt atcgtcggtc tgattattag tctgggacca cggtcccact cgtatcgtcg
     7081 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat aatcagactg
     7141 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccat ggtcccactc
     7201 gtatcgtcgg tctgattatt agtctgggac cacggtccca ctcgtatcgt cggtctgatt
     7261 attagtctgg aaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
     7321 gtcccactcg tatcgtcggt ctgattatta gtctgggacc acgatcccac tcgtgttgtc
     7381 ggtctgatta tcggtctggg accacggtcc cacttgtatt gtcgatcaga ctatcagcgt
     7441 gagactacga ttccatcaat gcctgtcaag ggcaagtatt gacatgtcgt cgtaacctgt
     7501 agaacggagt aacctcggtg tgcggttgta tgcctgctgt ggattgctgc tgtgtcctgc
     7561 ttatccacaa cattttgcgc acggttatgt ggacaaaata cctggttacc caggccgtgc
     7621 cggcacgtta accgggctgc atccgatgca agtgtgtcgc tgtcgacgag ctcgcgagct
     7681 cggacatgag gttgccccgt attcagtgtc gctgatttgt attgtctgaa gttgttttta
     7741 cgttaagttg atgcagatca attaatacga tacctgcgtc ataattgatt atttgacgtg
     7801 gtttgatggc ctccacgcac gttgtgatat gtagatgata atcattatca ctttacgggt
     7861 cctttccggt gatccgacag gttacggggc ggcgacctcg cgggttttcg ctatttatga
     7921 aaattttccg gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa
     7981 aataccctct gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc
     8041 gtttcctttc tctgtttttg tccgtggaat gaacaatgga agtccgagct catcgctaat
     8101 aacttcgtat agcatacatt atacgaagtt atattcgat
//
