LOCUS       40924_9241        8128 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCC1BAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   EPICENTRE Biotechnologies.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA
REFERENCE   2  (bases 1 to 8128)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8128)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-2007) 726 Post Road, Madison, WI 53713, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     288..304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        311..329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    332..388
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(431..447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(455..471)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(479..509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(524..545)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(768..1424)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1425..1527)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2386..3000
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      3076..3295
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             3386..4138
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    4144..4394
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             4720..5892
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             5895..6863
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    6939..7412
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    complement(7672..8070)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(8088..8121)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
       61 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccacacagat
      121 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt cagctgcgca actgttggga
      181 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      241 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      301 cagtgaattg taatacgact cactataggg cgaattcgag ctcggtaccc ggggatcctc
      361 tagagtcgac ctgcaggcat gcaagcttga gtattctata gtctcaccta aatagcttgg
      421 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      481 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      541 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      601 attaatgaat cggccaacgc gaaccccttg cggccgcccg ggccgtcgac caattctcat
      661 gtttgacagc ttatcatcga atttctgcca ttcatccgct tattatcact tattcaggcg
      721 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
      781 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      841 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      901 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      961 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     1021 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     1081 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     1141 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     1201 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1261 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1321 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1381 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1441 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1501 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     1561 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     1621 ggtatttatt cgcgataagc tcatggagcg gcgtaaccgt cgcacaggaa ggacagagaa
     1681 agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg aggcggttgc
     1741 cgccgctgct gctgacggtg tgacgttctc tgttccggtc acaccacata cgttccgcca
     1801 ttcctatgcg atgcacatgc tgtatgccgg tataccgctg aaagttctgc aaagcctgat
     1861 gggacataag tccatcagtt caacggaagt ctacacgaag gtttttgcgc tggatgtggc
     1921 tgcccggcac cgggtgcagt ttgcgatgcc ggagtctgat gcggttgcga tgctgaaaca
     1981 attatcctga gaataaatgc cttggccttt atatggaaat gtggaactga gtggatatgc
     2041 tgtttttgtc tgttaaacag agaagctggc tgttatccac tgagaagcga acgaaacagt
     2101 cgggaaaatc tcccattatc gtagagatcc gcattattaa tctcaggagc ctgtgtagcg
     2161 tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg taacgaaaac gatttgaata
     2221 tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt tgaagtggag cggattatgt
     2281 cagcaatgga cagaacaacc taatgaacac agaaccatga tgtggtctgt ccttttacag
     2341 ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc tcgagctggt tgccctcgcc
     2401 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     2461 gcgagacacc gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac
     2521 tgacagatga ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac
     2581 agatgagggg caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg
     2641 cgaaaacgcc tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag
     2701 tgccctgcgg tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt
     2761 gacacttgag gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct
     2821 gtccacaggc agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta
     2881 acctgtcttt taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg
     2941 cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc
     3001 ggtcgagtga gcgaggaagc accagggaac agcacttata tattctgctt acacacgatg
     3061 cctgaaaaaa cttcccttgg ggttatccac ttatccacgg ggatattttt ataattattt
     3121 tttttatagt ttttagatct tcttttttag agcgccttgt aggcctttat ccatgctggt
     3181 tctagagaag gtgttgtgac aaattgccct ttcagtgtga caaatcaccc tcaaatgaca
     3241 gtcctgtctg tgacaaattg cccttaaccc tgtgacaaat tgccctcaga agaagctgtt
     3301 ttttcacaaa gttatccctg cttattgact cttttttatt tagtgtgaca atctaaaaac
     3361 ttgtcacact tcacatggat ctgtcatggc ggaaacagcg gttatcaatc acaagaaacg
     3421 taaaaatagc ccgcgaatcg tccagtcaaa cgacctcact gaggcggcat atagtctctc
     3481 ccgggatcaa aaacgtatgc tgtatctgtt cgttgaccag atcagaaaat ctgatggcac
     3541 cctacaggaa catgacggta tctgcgagat ccatgttgct aaatatgctg aaatattcgg
     3601 attgacctct gcggaagcca gtaaggatat acggcaggca ttgaagagtt tcgcggggaa
     3661 ggaagtggtt ttttatcgcc ctgaagagga tgccggcgat gaaaaaggct atgaatcttt
     3721 tccttggttt atcaaacgtg cgcacagtcc atccagaggg ctttacagtg tacatatcaa
     3781 cccatatctc attcccttct ttatcgggtt acagaaccgg tttacgcagt ttcggcttag
     3841 tgaaacaaaa gaaatcacca atccgtatgc catgcgttta tacgaatccc tgtgtcagta
     3901 tcgtaagccg gatggctcag gcatcgtctc tctgaaaatc gactggatca tagagcgtta
     3961 ccagctgcct caaagttacc agcgtatgcc tgacttccgc cgccgcttcc tgcaggtctg
     4021 tgttaatgag atcaacagca gaactccaat gcgcctctca tacattgaga aaaagaaagg
     4081 ccgccagacg actcatatcg tattttcctt ccgcgatatc acttccatga cgacaggata
     4141 gtctgagggt tatctgtcac agatttgagg gtggttcgtc acatttgttc tgacctactg
     4201 agggtaattt gtcacagttt tgctgtttcc ttcagcctgc atggattttc tcatactttt
     4261 tgaactgtaa tttttaagga agccaaattt gagggcagtt tgtcacagtt gatttccttc
     4321 tctttccctt cgtcatgtga cctgatatcg ggggttagtt cgtcatcatt gatgagggtt
     4381 gattatcaca gtttattact ctgaattggc tatccgcgtg tgtacctcta cctggagttt
     4441 ttcccacggt ggatatttct tcttgcgctg agcgtaagag ctatctgaca gaacagttct
     4501 tctttgcttc ctcgccagtt cgctcgctat gctcggttac acggctgcgg cgagcgctag
     4561 tgataataag tgactgaggt atgtgctctt cttatctcct tttgtagtgt tgctcttatt
     4621 ttaaacaact ttgcggtttt ttgatgactt tgcgattttg ttgttgcttt gcagtaaatt
     4681 gcaagattta ataaaaaaac gcaaagcaat gattaaagga tgttcagaat gaaactcatg
     4741 gaaacactta accagtgcat aaacgctggt catgaaatga cgaaggctat cgccattgca
     4801 cagtttaatg atgacagccc ggaagcgagg aaaataaccc ggcgctggag aataggtgaa
     4861 gcagcggatt tagttggggt ttcttctcag gctatcagag atgccgagaa agcagggcga
     4921 ctaccgcacc cggatatgga aattcgagga cgggttgagc aacgtgttgg ttatacaatt
     4981 gaacaaatta atcatatgcg tgatgtgttt ggtacgcgat tgcgacgtgc tgaagacgta
     5041 tttccaccgg tgatcggggt tgctgcccat aaaggtggcg tttacaaaac ctcagtttct
     5101 gttcatcttg ctcaggatct ggctctgaag gggctacgtg ttttgctcgt ggaaggtaac
     5161 gacccccagg gaacagcctc aatgtatcac ggatgggtac cagatcttca tattcatgca
     5221 gaagacactc tcctgccttt ctatcttggg gaaaaggacg atgtcactta tgcaataaag
     5281 cccacttgct ggccggggct tgacattatt ccttcctgtc tggctctgca ccgtattgaa
     5341 actgagttaa tgggcaaatt tgatgaaggt aaactgccca ccgatccaca cctgatgctc
     5401 cgactggcca ttgaaactgt tgctcatgac tatgatgtca tagttattga cagcgcgcct
     5461 aacctgggta tcggcacgat taatgtcgta tgtgctgctg atgtgctgat tgttcccacg
     5521 cctgctgagt tgtttgacta cacctccgca ctgcagtttt tcgatatgct tcgtgatctg
     5581 ctcaagaacg ttgatcttaa agggttcgag cctgatgtac gtattttgct taccaaatac
     5641 agcaatagca atggctctca gtccccgtgg atggaggagc aaattcggga tgcctgggga
     5701 agcatggttc taaaaaatgt tgtacgtgaa acggatgaag ttggtaaagg tcagatccgg
     5761 atgagaactg tttttgaaca ggccattgat caacgctctt caactggtgc ctggagaaat
     5821 gctctttcta tttgggaacc tgtctgcaat gaaattttcg atcgtctgat taaaccacgc
     5881 tgggagatta gataatgaag cgtgcgcctg ttattccaaa acatacgctc aatactcaac
     5941 cggttgaaga tacttcgtta tcgacaccag ctgccccgat ggtggattcg ttaattgcgc
     6001 gcgtaggagt aatggctcgc ggtaatgcca ttactttgcc tgtatgtggt cgggatgtga
     6061 agtttactct tgaagtgctc cggggtgata gtgttgagaa gacctctcgg gtatggtcag
     6121 gtaatgaacg tgaccaggag ctgcttactg aggacgcact ggatgatctc atcccttctt
     6181 ttctactgac tggtcaacag acaccggcgt tcggtcgaag agtatctggt gtcatagaaa
     6241 ttgccgatgg gagtcgccgt cgtaaagctg ctgcacttac cgaaagtgat tatcgtgttc
     6301 tggttggcga gctggatgat gagcagatgg ctgcattatc cagattgggt aacgattatc
     6361 gcccaacaag tgcttatgaa cgtggtcagc gttatgcaag ccgattgcag aatgaatttg
     6421 ctggaaatat ttctgcgctg gctgatgcgg aaaatatttc acgtaagatt attacccgct
     6481 gtatcaacac cgccaaattg cctaaatcag ttgttgctct tttttctcac cccggtgaac
     6541 tatctgcccg gtcaggtgat gcacttcaaa aagcctttac agataaagag gaattactta
     6601 agcagcaggc atctaacctt catgagcaga aaaaagctgg ggtgatattt gaagctgaag
     6661 aagttatcac tcttttaact tctgtgctta aaacgtcatc tgcatcaaga actagtttaa
     6721 gctcacgaca tcagtttgct cctggagcga cagtattgta taagggcgat aaaatggtgc
     6781 ttaacctgga caggtctcgt gttccaactg agtgtataga gaaaattgag gccattctta
     6841 aggaacttga aaagccagca ccctgatgcg accacgtttt agtctacgtt tatctgtctt
     6901 tacttaatgt cctttgttac aggccagaaa gcataactgg cctgaatatt ctctctgggc
     6961 ccactgttcc acttgtatcg tcggtctgat aatcagactg ggaccacggt cccactcgta
     7021 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt
     7081 agtctgggac cacggtccca ctcgtatcgt cggtctgata atcagactgg gaccacggtc
     7141 ccactcgtat cgtcggtctg attattagtc tgggaccatg gtcccactcg tatcgtcggt
     7201 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctgga
     7261 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
     7321 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
     7381 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
     7441 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
     7501 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
     7561 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
     7621 ccgggctgca tccgatgcaa gtgtgtcgct gtcgacgagc tcgcgagctc ggacatgagg
     7681 ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac gttaagttga
     7741 tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg tttgatggcc
     7801 tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc ctttccggtg
     7861 atccgacagg ttacggggcg gcgacctcgc gggttttcgc tatttatgaa aattttccgg
     7921 tttaaggcgt ttccgttctt cttcgtcata acttaatgtt tttatttaaa ataccctctg
     7981 aaaagaaagg aaacgacagg tgctgaaagc gagctttttg gcctctgtcg tttcctttct
     8041 ctgtttttgt ccgtggaatg aacaatggaa gtccgagctc atcgctaata acttcgtata
     8101 gcatacatta tacgaagtta tattcgat
//
