LOCUS       62056_4705       10395 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             496..1212
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      1234..1486
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1715..1739
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3039..3665
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4097..5167
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5236..5430
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5774..5914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6100..6688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6778..7569)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7994..8018
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8096..8270)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8313..9335)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9403..10080)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10271..10292
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10307..10337
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10345..10361
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10369..10385
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcataaa tgtaggtatt acctgtacat tttatttatt cgagaaaaac aaacaaacca
       61 aaacgatgca gcgatcacta ccggcgtcat ccggaattga aacggcgcgc gttgaggggg
      121 gccttcttgt tcggacacac acggggccgc caaagtgggg cccatacggc tgcccatccc
      181 ttgctctcca ataccctggt catgtgactc gaaattggag actcacaaag atgctttgaa
      241 accccgccgg gaactttttc tcacagacac aatcagtcga ccttatcatc gcaaaatcga
      301 caaacctcaa aaagaactcg aagagtcagt ctcccttgcg gttaaactga ttgatatctc
      361 gtgttctcta aaccttcaat aagtgagtta ctgcgcacaa agccgactat actgagcacc
      421 aacgcgaatt gctcacgttg tcgcctaaca gatcttggct ttcgtaggaa cccaatcttc
      481 aaagagctcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
      541 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      601 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
      661 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
      721 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      781 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      841 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      901 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      961 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     1021 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     1081 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     1141 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     1201 gagctgtaca agtaggtcga cctgcaggca tgcgatcgtt caaacatttg gcaataaagt
     1261 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     1321 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     1381 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     1441 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcaagc ttggcactgg
     1501 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1561 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1621 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     1681 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     1741 taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa aaggtttatc
     1801 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     1861 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     1921 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     1981 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     2041 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     2101 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     2161 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     2221 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     2281 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     2341 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     2401 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     2461 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     2521 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     2581 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     2641 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     2701 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     2761 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     2821 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     2881 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     2941 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     3001 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     3061 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     3121 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     3181 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     3241 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     3301 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     3361 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     3421 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     3481 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     3541 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     3601 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     3661 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     3721 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     3781 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     3841 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     3901 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     3961 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     4021 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     4081 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     4141 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     4201 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     4261 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     4321 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     4381 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     4441 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     4501 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     4561 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     4621 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     4681 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     4741 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     4801 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     4861 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     4921 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     4981 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     5041 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     5101 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     5161 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     5221 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     5281 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     5341 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     5401 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     5461 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     5521 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     5581 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     5641 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     5701 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     5761 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     5821 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     5881 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     5941 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6001 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6061 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6121 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6181 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6241 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6301 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6361 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6421 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6481 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6541 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6601 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6661 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6721 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa
     6781 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
     6841 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
     6901 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
     6961 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
     7021 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
     7081 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
     7141 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
     7201 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
     7261 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
     7321 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
     7381 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
     7441 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
     7501 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
     7561 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
     7621 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
     7681 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
     7741 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     7801 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     7861 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     7921 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     7981 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     8041 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
     8101 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
     8161 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
     8221 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
     8281 tcgatcgaca gatccggtcg gcatctactc tatttctttg ccctcggacg agtgctgggg
     8341 cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt
     8401 ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat
     8461 cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg
     8521 tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc
     8581 ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag
     8641 atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct
     8701 gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc
     8761 cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac
     8821 gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg
     8881 catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac
     8941 ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt
     9001 agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg
     9061 gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg
     9121 agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag
     9181 ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct
     9241 tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc
     9301 tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc gggatctgcg
     9361 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
     9421 atgaaatgaa cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca
     9481 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
     9541 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     9601 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     9661 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     9721 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     9781 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc
     9841 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     9901 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     9961 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    10021 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    10081 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    10141 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt
    10201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10381 atgaccatga ttacg
//
