LOCUS       40924_8711       10549 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA-1302, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Hajdukiewicz P, Svab Z, Maliga P.
  TITLE     The small, versatile pPZP family of Agrobacterium binary vectors for
            plant transformation
  JOURNAL   Plant Mol. Biol. 25 (6), 989-994 (1994)
  PUBMED    7919218
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     A comprehensive set of modular vectors for advanced manipulations 
            and efficient transformation of plants
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10549)
  AUTHORS   Roberts C, Rajagopal S, Smith LM, Nguyen TA, Yang W, Nugrohu S, Ravi
            KS, Vijayachandra K, Harcourt RL, Dransfield L, Desamero N, Slamet 
            I, Hadjukiewicz P, Svab Z, Maliga P, Mayer JE, Keese PK, Kilian A, 
            Jefferson RA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / 
            GPO Box 3200, Canberra, ACT 2601, Australia
REFERENCE   4  (bases 1 to 10549)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "1994"; volume: "25"; issue: "6"; pages: "989-994"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 
            3200, Canberra, ACT 2601, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..727
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             734..751
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      783..1035
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1057..1081
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2381..3007
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3444..4508
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4577..4771
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5115..5255
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5441..6029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6119..6910)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7335..7359
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(7437..7611)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7654..8676)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8744..9421)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9612..9633
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9648..9678
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9686..9702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9710..9726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9736..9792
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9796..9812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10189..10534
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta
     1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     1501 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     1561 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     1621 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     1681 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     1741 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     1801 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     1861 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     1921 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     1981 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     2041 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     2101 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     2161 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     2221 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     2281 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     2341 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     2401 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     2461 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     2521 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     2581 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     2641 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     2701 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     2761 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     2821 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     2881 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     2941 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     3001 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     3061 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     3121 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     3181 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     3241 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     3301 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     3361 cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc
     3421 caagcccgag gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg
     3481 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     3541 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     3601 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     3661 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     3721 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     3781 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     3841 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     3901 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     3961 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     4021 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     4081 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     4141 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     4201 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     4261 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     4321 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     4381 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     4441 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     4501 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     4561 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     4621 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     4681 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     4741 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     4801 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     4861 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     4921 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     4981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     5161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     5221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
     6121 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
     6181 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
     6241 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
     6301 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
     6361 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
     6421 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
     6481 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
     6541 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
     6601 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
     6661 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
     6721 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
     6781 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
     6841 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
     6901 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
     6961 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
     7021 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
     7081 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
     7141 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
     7201 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
     7261 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
     7321 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
     7381 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgggggatc
     7441 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     7501 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     7561 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa actcgagctt
     7621 gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
     7681 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     7741 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     7801 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     7861 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
     7921 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     7981 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     8041 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     8101 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     8161 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     8221 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     8281 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
     8341 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     8401 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
     8461 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     8521 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     8581 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     8641 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cgggatctgc
     8701 gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca
     8761 aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc
     8821 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     8881 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     8941 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     9001 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     9061 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     9121 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc
     9181 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     9241 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     9301 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     9361 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     9421 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     9481 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt
     9541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     9601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     9721 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
     9781 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     9961 cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat
    10021 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct
    10081 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt
    10141 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
    10201 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
    10261 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
    10321 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    10381 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    10441 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
    10501 tatataagga agttcatttc atttggagag aacacggggg actcttgac
//
