LOCUS       V008809                 5797 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008809
VERSION     V008809
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5797)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5797)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(62..140)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(144..974)
                     /gene="Casp3"
                     /label="Caspase-3"
                     /note="Caspase-3 from Mus musculus. Accession#: P70677"
     misc_feature    complement(975..1041)
                     /label="5UTR"
                     /note="5UTR"
     polyA_signal    1154..1209
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1570..1586)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1594..1610)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1618..1648)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1662..1683)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1741..1937
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1943..2077
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2315..2903)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3077..3934)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3935..4039)
                     /label="AmpR promoter"
     enhancer        4070..4449
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4070..4447
                     /label="hCMV"
                     /note="hCMV"
     promoter        4451..4728
                     /label="chicken beta-actin promoter"
     intron          4729..5746
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gaaatgagag tctccctgac agctttctca tttggcataa caaaacagaa acacgccccc
      121 ccccaccccc aatcattcct ctagtgataa aagtacagtt ctttcgtgag catggacaca
      181 atacacggga tctgtttctt tgcgtggaaa gtggagtcca gggagaagga ctcgaattcc
      241 gttgccacct tcctgttaac gcgagtgaga atgtgcataa attctagctt gtgcgcgtac
      301 agcttcagca tgctgcaaag ggactggatg aaccacgacc cgtcctttga atttctccag
      361 gaatagtaac caggtgctgt agagtaagca tacaggaagt cagcctccac cggtatcttc
      421 tggcaagcca tctcctcatc agtcccactg tctgtctcaa tgccacagtc cagctccgta
      481 ccccggcagg cctgaatgat gaagagtttc ggctttccag tcagactccg gcagtagtcg
      541 cctctgaaga agctagtcaa ctttttcagt tcaacaggcc catttgtccc ataaatgacc
      601 ccttcatcac catggcttag aatcacacac acaaagctgc tccttttgct atgatcttcc
      661 ttagaaacac tatccattaa ttccaaaatg tcttcacgag taagatcatt tttattcctg
      721 acttggtatt tcaggcccat gaatgtctct ctgaggttgg ctgcgtccac atccgtacca
      781 gagcgagatg acattccagt gctcttatgg aagttcttat tattaattat tatgcatatg
      841 cccatttcag gataatccat tttgtaacta ctgtccagat agatcccaga gtccactgac
      901 ttgctcccat gtatggtctt tacttcaaaa ttattaatgg attttgaatc cactgaggtt
      961 ttgttgttct ccatggtcac ttttcttagc gtaccgttcc aagctccccg ccgcaggaca
     1021 ctgcgctttg atcccaccag cctcgtgccg aattccccca atgccctggc tcacaaatac
     1081 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     1141 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     1201 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     1261 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     1321 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     1381 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     1441 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     1501 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     1561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1621 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
     1681 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     1741 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1801 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1861 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1921 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     1981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2041 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     2101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2221 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2281 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2341 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2401 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2461 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2521 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2581 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2641 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2701 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2761 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2821 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2881 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2941 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3001 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3061 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3121 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3181 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3241 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3301 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3361 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3421 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3481 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3541 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3601 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3661 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3721 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3781 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3841 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3901 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3961 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4021 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     4081 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4141 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4201 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4261 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4321 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4381 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4441 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     4501 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     4561 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     4621 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     4681 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg
     4741 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     4801 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     4861 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     4921 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     4981 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     5041 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     5101 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     5161 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     5221 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     5281 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     5341 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     5401 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     5461 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     5521 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     5581 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     5641 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     5701 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     5761 gctggttatt gtgctgtctc atcattttgg caaagaa
//
