LOCUS       V008817                 6282 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008817
VERSION     V008817
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6282)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(182..376)
                     /label="3UTR"
                     /note="3UTR"
     CDS             complement(380..1498)
                     /gene="Casp4"
                     /label="Caspase-4"
                     /note="Caspase-4 from Mus musculus. Accession#: P70343"
     misc_feature    complement(1499..1524)
                     /label="5UTR"
                     /note="5UTR"
     polyA_signal    1639..1694
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2055..2071)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2079..2095)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2103..2133)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2147..2168)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2226..2422
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2428..2562
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2800..3388)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3562..4419)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4420..4524)
                     /label="AmpR promoter"
     enhancer        4555..4934
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4555..4932
                     /label="hCMV"
                     /note="hCMV"
     promoter        4936..5213
                     /label="chicken beta-actin promoter"
     intron          5214..6231
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gcgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
      121 atctattcga tgatgaagat accccaccaa acccaaaaaa agagatcgaa ttcggcacga
      181 gttttttttt ttttttgggc ttgtttgctt gtctatggaa cagaatccca ctgtgttgct
      241 taggctggtc ttgaattcat aagtattctt tccttaattt cactagttct ggaatttcag
      301 gaatagaatg tgatccttgg ccaagatcac ttcttcaagc tgaagaaatg agattcagtt
      361 gcttgttgct ttgttctcag ttgccaggaa agaggtagaa atatctcgtc aaggttgccc
      421 gatcaatggt gggcatctgg gaatgaatac ttgccttttc aaatgattgt tgcaccttca
      481 ggaatatatc aaagagatga caagagcaag catgtttccg gaagcaggaa atgagtctag
      541 tgatgaagta agagcctcct gttttgtctc ggtaggacaa gtgatgtggg gttgtagagt
      601 agaaggcaat gaagtccttc tccacgtggc tcagcttgac agcatcagct tccatattcc
      661 taggtagatc tacacctctg cacaactggg gttttgaaga ctctctgatc cacatttctc
      721 cagagttccc acctctgcag gcctgcacaa tgatgacttt gggtttgtct cgtagacctg
      781 gacagtggca attgttgaat atctgataga tggtatcata ctgtagcaca tctggagttt
      841 tttcactgtg cattgttcca caaatgccat gcagtgtgcc atgagacatt agcaccagga
      901 atgtgctgtc tgatgtctgg tgttctgaga gtgcagcaaa gtctttcatc tctgactcca
      961 tgccctctgc tgtaagctcc tctttcacca ccacatcgta gcctaagtct tcaagaaggc
     1021 ctttcatacc aatgatgtca aagttagccc catacctcag tgagagatgt ttgaactctg
     1081 tattgcatat gataagagcc tttcgtgtac ggccattggc ctcctttatt gggtaaattt
     1141 cttgtgtctt ttctctgcaa agccttgtga actcttcagg ggaacaaagc ttgagagtgt
     1201 tcaatgattc ttctggttcc tccatttcca gattagcttc accatggtgg ctgcctgggt
     1261 ccacactgaa gaatgtctgg agaagcattt cacctacttt gctgtgtttc tttttcatgg
     1321 catctacaaa aacccaacgc ttgtcactgc gttcagcatt gttaaatttt tgtttatctt
     1381 cctcctttaa tttcagtaca ttgctttgta ctaatttttc taggtactcc gtaaggactt
     1441 ctttgcccag ctgttccaac accttaagtg gtttgtcagg gtgtttgttt tcagccatga
     1501 gaaaaagcct cagttctttc gcaccgctcg tgccgaattc ccccaatgcc ctggctcaca
     1561 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     1621 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     1681 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     1741 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     1801 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     1861 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     1921 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     1981 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     2041 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     2101 cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac
     2161 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2221 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2281 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2341 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2401 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     2461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     2581 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2641 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2701 tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt
     4561 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     4621 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     4681 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     4741 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     4801 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     4861 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     4921 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
     4981 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
     5041 cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg
     5101 cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     5161 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     5221 ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     5281 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     5341 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     5401 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     5461 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     5521 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     5581 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     5641 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     5701 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     5761 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     5821 ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt
     5881 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     5941 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     6001 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     6061 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     6121 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     6181 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     6241 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//
