LOCUS       V008802                 7426 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008802
VERSION     V008802
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Paterson RG, Russell CJ, Lamb RA.
  TITLE     Fusion protein of the paramyxovirus SV5: destabilizing and
            stabilizing mutants of fusion activation
  JOURNAL   Virology 270 (1), 17-30 (2000)
   PUBMED   10772976
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7426)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes
            Medical Institute and Massachusetts Institute of Technology, 43
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 7426)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology";
            date: "2000"; volume: "270"; issue: "1"; pages: "17-30"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical
            Institute and Massachusetts Institute of Technology, 43 Vassar St.,
            Cambridge, MA 02139, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..645
                     /label="chicken beta-actin promoter"
     intron          647..1664
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1755..4403
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     polyA_signal    4495..4550
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4911..4927)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4935..4951)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4959..4989)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5004..5025)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5083..5279
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    5285..5419
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5658..6246)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6420..7277)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7278..7382)
                     /label="AmpR promoter"
     promoter        7410..7426
                     /label="CAG"
                     /note="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
      661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1681 tggttattgt gctgtctcat cattttggca aagaattcga gctcatcgat gcatggtacc
     1741 cgggcatgct cgagatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac
     1801 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac
     1861 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg
     1921 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta
     1981 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc
     2041 gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt
     2101 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg
     2161 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg
     2221 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc
     2281 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac
     2341 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct
     2401 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg
     2461 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa
     2521 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta
     2581 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc
     2641 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg
     2701 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag
     2761 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg
     2821 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg
     2881 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta
     2941 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt
     3001 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag
     3061 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag
     3121 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc
     3181 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc
     3241 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct
     3301 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt
     3361 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg
     3421 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta
     3481 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta
     3541 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac
     3601 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga
     3661 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc
     3721 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat
     3781 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa
     3841 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg
     3901 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt
     3961 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc
     4021 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt
     4081 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag
     4141 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta
     4201 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat
     4261 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt
     4321 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct
     4381 tagagtcgga cttcgcgttc gcgtaacgcc aaatcaatac gactccggat ctttttccct
     4441 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     4501 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     4561 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     4621 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     4681 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     4741 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     4801 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     4861 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     4921 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4981 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5041 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     5101 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5161 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5221 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     5281 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5401 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5461 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7381 cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattg
//
