LOCUS       40924_8396        5464 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAGmA20f7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5464)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             49..738
                     /codon_start=1
                     /note="unnamed protein product; mA20 (547-775)"
                     /protein_id="SJL88082.1"
                     /translation="RSKSDPSQLIQSLTPHSCHRTGNVSPSGCLSQAARTPGDRAGTSK
                     CRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASAKAGSPAPRFQNNVPCLGRECGTL
                     GSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRTTQVASRPKCARASCKNI
                     LACRSEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACDHFGNAKCNGYCNECY
                     QFKQMYG"
     polyA_signal    817..872
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1233..1249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1257..1273)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1281..1311)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1325..1346)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1404..1600
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1606..1740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1978..2566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2740..3597)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3598..3702)
                     /label=AmpR promoter
     enhancer        3737..4116
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3737..4114
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4118..4395
                     /label=chicken beta-actin promoter
     intron          4396..5413
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgcg ctccaagtct
       61 gacccctcac aactcatcca aagtctcact ccacactctt gccaccggac tggaaatgtc
      121 tctccttctg gctgcctctc ccaggctgca cggactccag gagacagagc agggacaagc
      181 aagtgcagga aagctggctg catgtatttt gggactccag aaaacaaggg cttttgcact
      241 ctatgtttca tcgaatacag agaaaataag cagtctgtta ctgcctctgc gaaagctggt
      301 tccccggccc ccaggttcca gaacaatgtc ccgtgcctgg gcagggagtg cggcacactc
      361 ggaagcacca tgtttgaagg gtactgtcag aagtgtttca tcgaagctca gaaccagaga
      421 ttccatgaag caagaagaac ggaagaacag ctgagatcaa gccagcatag agacatgcct
      481 cgaactacac aggtagcctc aaggccgaaa tgtgcccggg cctcctgcaa gaacattctg
      541 gcctgtcgca gtgaggaact ctgtatggag tgccagcacc taagccaacg agtaggttct
      601 gtggcccacc ggggtgagcc cacgcctgaa gagcccccta aacagcgctg ccgggcccct
      661 gcttgtgatc actttggcaa tgccaagtgt aatggttact gcaatgagtg ctaccagttc
      721 aagcagatgt atggctaagt gtcgacgaag atctttttcc ctctgccaaa aattatgggg
      781 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
      841 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
      901 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
      961 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     1021 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     1081 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     1141 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     1201 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
     1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     1441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     1621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1741 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
     1921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3721 gccacctggg tcgatcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3781 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     3841 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3901 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3961 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     4021 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     4081 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     4141 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     4201 ttattttgtg cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg
     4261 cggggcgagg ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc
     4321 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     4381 agcgcgcggc gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc
     4441 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     4501 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     4561 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     4621 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     4681 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     4741 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     4801 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     4861 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     4921 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     4981 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg
     5041 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     5101 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     5161 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     5221 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     5281 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5341 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     5401 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     5461 agaa
//
