LOCUS       40924_8356        5747 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAGAVPShOMI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5747)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             43..1023
                     /codon_start=1
                     /note="unnamed protein product; mature hOMI"
                     /protein_id="SJL87285.1"
                     /translation="MAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREV
                     PISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQT
                     KEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYI
                     QTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGI
                     SGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVI
                     LAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE"
     polyA_signal    1100..1155
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1516..1532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1540..1556)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1564..1594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1608..1629)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1687..1883
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1889..2023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2261..2849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3023..3880)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3881..3985)
                     /label=AmpR promoter
     enhancer        4020..4399
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4020..4397
                     /label=hCMV enhancer
                     /note="hCMV enhancer"
                     /regulatory_class="promoter"
     promoter        4401..4678
                     /label=chicken beta-actin promoter
     intron          4679..5696
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggccgt ccctagcccg
       61 ccgcccgctt ctccccggag tcagtacaac ttcatcgcag atgtggtgga gaagacagca
      121 cctgccgtgg tctatatcga gatcctggac cggcaccctt tcttgggccg cgaggtccct
      181 atctcgaacg gctcaggatt cgtggtggct gccgatgggc tcattgtcac caacgcccat
      241 gtggtggctg atcggcgcag agtccgtgtg agactgctaa gcggcgacac gtatgaggcc
      301 gtggtcacag ctgtggatcc cgtggcagac atcgcaacgc tgaggattca gactaaggag
      361 cctctcccca cgctgcctct gggacgctca gctgatgtcc ggcaagggga gtttgttgtt
      421 gccatgggaa gtccctttgc actgcagaac acgatcacat ccggcattgt tagctctgct
      481 cagcgtccag ccagagacct gggactcccc caaaccaatg tggaatacat tcaaactgat
      541 gcagctattg attttggaaa ctctggaggt cccctggtta acctggatgg ggaggtgatt
      601 ggagtgaaca ccatgaaggt cacagctgga atctcctttg ccatcccttc tgatcgtctt
      661 cgagagtttc tgcatcgtgg ggaaaagaag aattcctcct ccggaatcag tgggtcccag
      721 cggcgctaca ttggggtgat gatgctgacc ctgagtccca gcatccttgc tgaactacag
      781 cttcgagaac caagctttcc cgatgttcag catggtgtac tcatccataa agtcatcctg
      841 ggctcccctg cacaccgggc tggtctgcgg cctggtgatg tgattttggc cattggggag
      901 cagatggtac aaaatgctga agatgtttat gaagctgttc gaacccaatc ccagttggca
      961 gtgcagatcc ggcggggacg agaaacactg accttatatg tgacccctga ggtcacagaa
     1021 tgagtcgacg aagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     1081 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     1141 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     1201 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     1261 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     1321 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     1381 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     1441 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     1501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1561 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa
     1621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1681 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     1741 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1801 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1861 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     1921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1981 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2161 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc
     2221 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2281 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2341 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2401 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2461 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2521 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2581 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2641 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2701 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2881 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2941 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3001 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3061 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3121 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3181 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3241 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3301 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3361 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3421 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3481 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3541 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3601 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3661 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3721 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3781 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3841 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3901 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3961 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg
     4021 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4081 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4381 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     4441 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     4501 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
     4561 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     4621 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     4681 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     4741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     4801 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     4861 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     4921 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     4981 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     5041 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     5101 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     5161 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     5221 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     5281 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
     5341 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     5401 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     5461 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     5521 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     5581 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     5641 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     5701 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//
