LOCUS       V008877                 6271 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008877
VERSION     V008877
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label="E-tag"
                     /note="E-tag"
     CDS             59..1465
                     /gene="PELI3"
                     /label="E3 ubiquitin-protein ligase pellino homolog 3"
                     /note="E3 ubiquitin-protein ligase pellino homolog 3 from
                     Homo sapiens. Accession#: Q8N2H9"
     polyA_signal    1623..1678
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2039..2055)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2063..2079)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2087..2117)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2131..2152)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2210..2406
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2412..2546
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2784..3372)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3546..4403)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4404..4508)
                     /label="AmpR promoter"
     enhancer        4539..4918
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4539..4916
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4920..5197
                     /label="chicken beta-actin promoter"
     intron          5198..6215
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat
       61 ggtgctggaa ggaaaccctg aagtggggtc cccccgaacc tcagacctcc agcaccgggg
      121 gaacaagggc tcttgcgttc tctcctctcc cggtgaagat gcgcagccag gcgaggagcc
      181 catcaagtat ggtgaactca tcgtcctggg atgctgtgag gaaggaggtg aggaaaccga
      241 ggctcagaga ggggaagtga ctggcccaag ggcacacagc tgctacaatg gttgtctggc
      301 aagtggggac aagggccgcc ggcgaagccg cctggcactg agccgccggt cgcacgccaa
      361 cggggtgaag ccagacgtca tgcaccacat ctccacgccg ctcgtctcca aggcactgag
      421 taaccgtggt cagcacagca tctcgtatac actgtcccgg agccactcgg tcatagtgga
      481 gtatacacat gatagcgaca cagacatgtt ccagattggc cgctccacag agaacatgat
      541 tgacttcgtg gtaacagaca cgtcccctgg aggaggggct gccgagggcc cttctgccca
      601 gagcaccatc tcccgctatg cctgccgcat cctctgtgac cgccggccac cctatactgc
      661 ccgcatctat gccgctggct tcgacgcctc tagcaacatc ttccttggag agcgagcggc
      721 caaatggcgg accccagatg gcctgatgga tggactgacc accaatggag tcctggtgat
      781 gcacccggca ggcggcttct ccgaggactc agccccgggt gtctggcggg agatctcggt
      841 ctgtgggaat gtgtacacat tgcgggacag ccgctcagcc cagcagcggg gcaagctggt
      901 ggaaaacgag tccaacgtgc tgcaggacgg ctctctcatc gacctgtgtg gggccacact
      961 gctgtggcgc acaccggcgg ggctgctgcg ggctcccaca ctgaagcaac tggaggccca
     1021 gcggcaggag gcaaatgcag cgcggcccca gtgccccgtg ggcctcagca ctctggcctt
     1081 ccccagccca gcccgtggcc gcacagcgcc cgacaaacag cagccctggg tctacgtccg
     1141 ctgcgggcac gtccatggct accacggctg gggctgccgg cgggagcggg gcccccagga
     1201 gcgcgaatgt cctctctgcc gccttgtggg gccttatgtg cctctatggc ttggccagga
     1261 ggccggcctc tgcctggacc ctgggccgcc tagccatgcc tttgcacctt gcggccacgt
     1321 ctgctctgag aagactgccc gctactgggc ccagacacca ctgccccacg gcacccatgc
     1381 tttccatgcc gcctgcccct tttgcggggc ctggcttacc ggcgagcatg gctgcgtccg
     1441 cctcattttc cagggcccgc tggattagct cgaggaattc actcctcagg tgcaggctgc
     1501 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     1561 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1621 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     1681 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1741 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1801 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1861 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1921 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1981 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     2041 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2101 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
     2161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     2221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2341 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2401 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2461 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2521 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2701 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4501 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     4561 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4621 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4681 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4741 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4801 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4861 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4921 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4981 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     5041 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
     5101 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     5161 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
     5221 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     5281 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     5341 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     5401 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     5461 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     5521 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     5581 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     5641 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     5701 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     5761 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     5821 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     5881 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5941 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     6001 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     6061 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     6121 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     6181 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     6241 tgctgtctca tcattttggc aaagaattga a
//
