LOCUS       40924_7951       11928 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pC35-STDParAo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Shao M, Blechl A, Thomson JG.
  TITLE     Small Serine Recombination Systems ParA-MRS and CinH-RS2 Perform 
            Precise Excision of Plastid DNA
  JOURNAL   Plant Biotechnol. J. (2017) In press
  PUBMED    28421718
REFERENCE   2  (bases 1 to 11928)
  AUTHORS   Shao M, Blechl A, Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2017) Crop Improvement and Utilization, USDA, 800 
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 11928)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JAN-2017) Crop Improvement and Utilization, USDA, 800 Buchanan 
            Street, Albany, CA 94710, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(82..106)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     309..325
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1571..1914
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      1933..1942
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1939..2790
                     /codon_start=1
                     /gene="STDParAo"
                     /product="STDParA"
                     /label=STDParAo
                     /note="site-specific recombinase ParA from INSD accession 
                     M59825; originally isolated from plasmids RP4 and RK2; 
                     plant codon optimized; modified to include tobacco gene for
                     ribulose 1,5-bisphosphate carboxylase small subunit stroma 
                     targeting domain (STD) from INSD accession X02353"
                     /protein_id="ARK38553.1"
                     /translation="MASSLLSSAAVATRSNVAQANMVAPFTGLKSAASFPVSRKQNLDI
                     TSIASNGGRVQCMLINMATREQQPEGRRLKVARIYLRASTDEQNLERQESLVAATRAAG
                     YYVAGIYREKASGARADRPELLRMIADLQPGEVVVAEKIDRISRLPLAEAERLVASIRA
                     KGAKLAVPGVVDLSELAAEANGVAKIVLESVQDMLLKLALQMARDDYEDRRERQRQGVQ
                     LAKAAGRYTGRKRDAGMHDRIIALRSGGSSIAKTAKLVGCSPSQVKRVWAAWNAQQQNK
                     RA"
     gene            1939..2790
                     /gene="STDParAo"
                     /label=STDParAo
     terminator      2965..3217
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3581..3597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3605..3621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3629..3659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3674..3695)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3886..4563
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             4632..5420
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5480..5654
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(5732..5756)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6181..6972
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7062..7650
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7836..7976)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8320..8514)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8583..9647)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10084..10710)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
       61 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
      121 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
      181 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      241 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      301 acgacgttgt aaaacgacgg ccagtgccaa gcttagcaag agctaacggc tcttcgcaac
      361 aaggagacct ccctctccac cgtgaaacac cggtaaaaga agaagcagtt cattctgcac
      421 tggccacttt tacgccaact caagtaaagg ctattccaga gcaaacggct cctggtaaag
      481 aatcaacaaa tccgttgatg gctagtatct tgccaaaaga tatgaaccca gttcaaactg
      541 ggataaggct tgcagtgcca ggggactttt tacgtcctca tcagggaatt ccaatcccac
      601 aaaaatctga gcttagcagc atagttgctc ctctcagagc agaatcgggt attcaccacc
      661 ctcatatcaa ctactacgtt gtgtataacg gtccacacgc cggtatatac gatgactggg
      721 gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc atacaagaag tttgccacta
      781 ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga
      841 acttcatccc caaaggagaa gctcaactca agcccaagag ctttgcgaag gccttaacca
      901 gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc
      961 cagccccaaa agagatctcc tttgccccgg agatcaccat ggacgacttt ctctatctct
     1021 acgatctagg aagaaagttc gacggagaag gtgacgatac catgttcacc accgataatg
     1081 agaagattag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     1141 acgcggcagg tctcatcaag acgatctacc cgagtaataa tctccaggag atcaaatacc
     1201 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
     1261 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     1321 ttcataaacc aaggcaagta atagagattg gagtctctaa gaaagtagtt cctactgaat
     1381 caaaggccat ggagtcaaaa attcagatcg aggatctaac agaactcgcc gtgaagactg
     1441 gcgaacagtt catacagagt cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
     1501 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     1561 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     1621 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
     1681 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
     1741 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
     1801 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
     1861 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggatctgct
     1921 ctagggcgcg ccgccaccat ggcttcctca cttctttcct ctgcagcagt tgccacccgc
     1981 agcaatgttg ctcaagctaa catggttgca cctttcactg gccttaagtc agctgcctca
     2041 ttccctgttt caaggaagca aaaccttgac atcacttcca ttgccagcaa cggcggaaga
     2101 gtgcaatgca tgttaattaa catggcgacc agggagcaac aaccggaggg cagacgtctc
     2161 aaggtcgcta gaatctacct ccgggctagt actgacgagc aaaatcttga aaggcaagag
     2221 tcgctggtcg ctgcgaccag agctgccggg tattacgttg caggaatcta ccgtgaaaaa
     2281 gcctcaggag ccagagcaga caggccagaa ctcctgagaa tgatcgccga tctccaacct
     2341 ggcgaggtgg tggtggcgga gaagatcgat cgcattagta gattgccgct cgcggaggcc
     2401 gagcgcctcg ttgcgagtat tcgcgctaag ggtgcgaagc tcgctgtccc gggagtcgtg
     2461 gacttgtccg agctcgccgc agaggccaac ggtgtcgcta agatcgtgct cgaaagcgtg
     2521 caagatatgc tactgaagct ggctctccaa atggctcgcg acgattacga ggaccggagg
     2581 gagcgccaac ggcagggcgt gcagctcgct aaggctgctg gcaggtacac cggcaggaag
     2641 cgtgatgctg ggatgcatga ccgcattatt gccctccgga gtgggggttc tagcatcgcg
     2701 aagaccgcta aactggttgg atgcagcccg tcccaggtta agagggtttg ggccgcctgg
     2761 aacgcccagc agcaaaacaa gagggcttag actagtgcat gcattcatca atattattca
     2821 tgcggggaaa ggcaagatta atccaactgg caaatcatcc agcgtgattg gtaacttcag
     2881 ttccagcgac ttgattcgtt ttggtgctac ccacgttttc aataaggacg agatggtgga
     2941 gtaaagaagg agtgcgtcga agcagatcgt tcaaacattt ggcaataaag tttcttaaga
     3001 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     3061 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     3121 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     3181 aaattatcgc gcgcggtgtc atctatgtta ctagatcgat caaacttcgg cactgtgtaa
     3241 tgacgatgag caatcgagag gctgactaac aaaaggtatg cccaaaaaca acctctccaa
     3301 actgtttcga attggaagtt tctgctcatg ccgacaggca taacttagat attcgcgggc
     3361 tattcccact aattcgtcct gctggtttgc gccaagataa atcagtgcat ctccttacaa
     3421 gttcctctgt cttgtgaaat gaactgctga ctgcccccca agaaagcctc ctcatctccc
     3481 agttggcggc ggctgataca ccatcgaaaa cccacgtccg aacacttgat acatgtgcct
     3541 gagaaatagg ggtcgacccg agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt
     3601 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3661 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3721 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3781 gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca ctctcgtcta
     3841 ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
     3901 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa
     3961 aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc
     4021 tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
     4081 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa
     4141 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     4201 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     4261 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     4321 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     4381 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     4441 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
     4501 ctatccttcg caagaccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     4561 tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag atctgtcgat
     4621 cgaccatggg gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4681 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4741 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4801 atgaactcca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4861 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4921 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4981 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5041 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5101 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5161 tgcccgacgg cgaggatctc gtcgtgacac atggcgatgc ctgcttgccg aatatcatgg
     5221 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5281 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5341 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5401 gccttcttga cgagttcttc tgagcgggac tctggggttc ggatcgatcc tctagctaga
     5461 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     5521 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     5581 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     5641 ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca
     5701 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     5761 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     5821 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     5881 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     5941 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg
     6001 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
     6061 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     6121 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     6181 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     6241 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     6301 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     6361 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     6421 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     6481 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     6541 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     6601 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     6661 gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     6721 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     6781 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     6841 attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta
     6901 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     6961 gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga gttttcgttc
     7021 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7681 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7741 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7801 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     7861 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     7921 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     7981 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8041 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8101 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
     8161 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
     8221 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
     8281 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
     8341 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
     8401 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
     8461 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
     8521 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
     8581 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
     8641 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
     8701 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
     8761 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
     8821 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
     8881 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
     8941 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
     9001 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
     9061 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
     9121 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
     9181 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
     9241 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
     9301 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
     9361 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
     9421 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
     9481 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
     9541 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
     9601 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc
     9661 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc
     9721 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
     9781 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
     9841 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
     9901 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
     9961 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
    10021 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
    10081 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
    10141 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
    10201 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
    10261 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
    10321 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
    10381 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
    10441 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
    10501 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
    10561 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
    10621 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
    10681 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
    10741 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
    10801 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
    10861 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
    10921 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
    10981 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
    11041 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
    11101 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
    11161 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
    11221 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
    11281 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
    11341 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
    11401 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
    11461 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
    11521 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
    11581 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
    11641 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
    11701 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
    11761 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
    11821 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
    11881 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatg
//
