LOCUS       V009148                 6926 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009148
VERSION     V009148
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6926)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             194..2053
                     /gene="Gucy1b1"
                     /label="Guanylate cyclase soluble subunit beta-1"
                     /note="Guanylate cyclase soluble subunit beta-1 from Mus
                     musculus. Accession#: O54865"
     CDS             2095..2136
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             2146..2163
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    2188..2320
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_recomb     2333..3038
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        3611..3715
                     /label="AmpR promoter"
     CDS             3716..4573
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4747..5335
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5512..6624)
                     /codon_start=1
                     /note="unnamed protein product; 5' lacZ fragment"
                     /protein_id="SJL87778.1"
                     /translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
     regulatory      complement(6625..6899)
                     /label="AcNPV-ETL promoter"
                     /note="AcNPV-ETL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
      181 tgcccttgac accatgtacg gtttcgtgaa ccatgccctg gagctgctgg tgatccgcaa
      241 ttatggtccc gaggtgtggg aagacatcaa aaaagaggca cagctggatg aagaaggcca
      301 gtttcttgtc agaataatat acgatgattc caaaacatat gacctggtgg ctgctgcaag
      361 caaagtcctc aacctcaatg ctggcgaaat cctgcagatg tttgggaaga tgtttttcgt
      421 cttctgccag gagtctggct atgataccat cttgcgtgtc ctgggatcta atgtcagaga
      481 gtttttgcag aacctcgatg ccctgcatga ccacctcgcc accatttacc cagggatgcg
      541 cgcgccttcc ttcaggtgca ccgatgcgga gaaaggcaaa gggctcatcc tgcactacta
      601 ctcggaaaga gaggggcttc aggacatcgt gatcgggatt atcaagactg ttgctcaaca
      661 gatacacggc actgagatag acatgaaggt gattcagcaa agaaatgaag aatgtgatca
      721 cacccagttt ttaattgaag aaaaggaatc aaaagaagag gatttttatg aagatctgga
      781 taggtttgaa gagaatggta cccaggaatc acgtatcagc ccttacacct tctgcaaagc
      841 atttcctttt cacatcatat ttgaccggaa cctagtggtc actcagtgtg gcaatgccat
      901 ctacagagtg ctcccccagc tccagcctgg gaactgcagc cttctgtctg tcttctctct
      961 ggtccgccct cacatcgaca tcagtttcca tgggattctt tcacacatca atacagtctt
     1021 tgtactgaga agcaaggaag ggttgctgga tgtggagaaa cttgagtgtg aggatgaact
     1081 gactggagca gagattagct gcttacgtct caaaggccaa atgatctact taccagaagc
     1141 agatagcatc ctcttcctct gttcaccaag tgtgatgaac ctggacgacc taacaagaag
     1201 aggcctgtat ctgagtgaca tccctctcca cgatgctacc cgagacctgg ttcttttggg
     1261 agaacagttc cgggaggagt acaaactgac acaagagctg gaaatcctca ccgacaggct
     1321 gcagctcaca ctgagagcct tggaggatga gaagaaaaag acagacacat tgctgtattc
     1381 tgtccttcct ccatctgttg ccaatgagct gagacacaag cgcccagtgc ctgccaaaag
     1441 atacgacaat gtgaccatcc tcttcagcgg cattgtgggc ttcaatgctt tctgtagcaa
     1501 gcatgcatct ggagaagggg ccatgaagat tgtcaatctc ctcaacgatc tctacacccg
     1561 atttgacaca ctgactgatt cacgaaaaaa cccatttgtt tacaaggtgg aaacagttgg
     1621 tgacaagtat atgacagtga gtggcttgcc agaaccttgt atccaccatg cacggtccat
     1681 ttgccacctg gctttagaca tgatggaaat tgctggtcaa gttcaagtag atggtgaatc
     1741 tgttcagata acaatcggga tccataccgg ggaggtggtg acaggtgtga ttggacagcg
     1801 gatgcctcgg tattgtctct ttgggaatac cgtcaacctc acaagcagga cagaaaccac
     1861 aggagaaaag ggaaagataa atgtttccga atatacatac aggtgtctca tgtctccaga
     1921 aaactcggat ccactgttcc atttggagca cagaggccca gtgtctatga agggcaagaa
     1981 ggaaccaatg caagtctggt tcctatccag gaaaaataca ggcacggagg aaacaaatga
     2041 ggaggatgaa aactgagtgt caggttgtgg aagggcaatt cgaagcttag gcctggtaag
     2101 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     2161 cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa tggttacaaa
     2221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2281 ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa gaggaggaaa
     2341 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt
     2401 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag
     2461 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
     2521 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
     2581 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
     2641 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
     2701 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
     2761 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
     2821 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca tatttaacat
     2881 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
     2941 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
     3001 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt
     3061 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg
     3121 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg
     3181 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag
     3241 gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg
     3301 gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa tctgctctga
     3361 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3421 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3481 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     3541 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     3601 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     3661 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     3721 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     3781 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3841 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     3901 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3961 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     4021 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     4081 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     4141 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     4201 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     4261 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     4321 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     4381 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     4441 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     4501 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     4561 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     4621 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     4681 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4741 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4801 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4861 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4921 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4981 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5041 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5101 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     5161 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     5221 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     5281 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     5341 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5401 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     5461 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag catcctgcac
     5521 catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc
     5581 tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg
     5641 gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac
     5701 caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag
     5761 acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg
     5821 cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta
     5881 gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc
     5941 attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga
     6001 gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc
     6061 actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag
     6121 gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca
     6181 cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca
     6241 gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa
     6301 cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat cgtaaccgtg
     6361 catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca
     6421 gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct
     6481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6601 ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct gctctttgat
     6661 acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca tgtttgcaat
     6721 cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg ctggacacca
     6781 cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa tgcaaacttt
     6841 ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag cgagtgcctt
     6901 cgactttttc gtgtccagtg tggctt
//
