LOCUS       40924_6721        4861 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..94
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             110..127
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             131..163
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             167..190
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_recomb     265..970
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1546..1650
                     /label=AmpR promoter
     CDS             1651..2508
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2682..3270
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaacctat aaatatgccg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga
      181 tgacgataag gatcgatgga tccgacctcg agatctgcag ctggtaccat ggaattcgaa
      241 gcttggagtc gactctgctg aagaggagga aattctcctt gaagtttccc tggtgttcaa
      301 agtaaaggag tttgcaccag acgcacctct gttcactggt ccggcgtatt aaaacacgat
      361 acattgttat tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa
      421 ttgttgtacg tattttaata attcattaaa tttataatct ttagggtggt atgttagagc
      481 gaaaatcaaa tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga
      541 tttgtaaaat aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg
      601 actatctaat ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct
      661 agctttgtcg atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat
      721 attatgcgct tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa
      781 cacgttaaat aaagcctgga catatttaac atcgggcgtg ttagctttat taggccgatt
      841 atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc
      901 cacacgacgc ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct
      961 ttttggaatt atttctgatt gcgggcgttt ttgggcgggt ttcaatctaa ctgtgcccga
     1021 ttttaattca gacaacacgt tagaaagcga tggtgcaggc ggtggtaaca tttcagacgg
     1081 caaatctact aatggcggcg gtggtggagc tgatgataaa tctaccatcg gtggaggcgc
     1141 aggcggggct ggcggcggag gcggaggcgg aggtggtggc ggtgatgcag acggcggttt
     1201 aggctcaaat tgtctctttc aggcaacaca gtcggcacct caactattgt actggtttcg
     1261 ggcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     1321 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1381 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1441 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1501 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1561 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1621 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1681 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1741 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1801 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1861 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1921 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1981 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2041 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2101 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2161 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2221 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2281 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2341 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2401 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2461 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2521 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2581 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2641 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2701 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2761 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2821 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2881 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2941 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3001 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3061 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3121 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3181 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3241 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3301 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3361 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3421 cgcagcgagt cagtgagcga ggaagcatcc tgcaccatcg tctgctcatc catgacctga
     3481 ccatgcagag gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc
     3541 agcagcagca gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct
     3601 tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt
     3661 tcggcgctcc acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggtataa
     3721 ccaccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt
     3781 tcaccctgcc ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga
     3841 acttcagcct ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa
     3901 tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc
     3961 cgccacatat cctgatcttc cagataactg ccgtcactcc aacgcagcac catcaccgcg
     4021 aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg
     4081 tcctggccgt aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca
     4141 tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc
     4201 gagtaacaac ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag
     4261 gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg
     4321 acagtatcgg cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc
     4381 ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4441 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4501 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggg atctatcatt
     4561 tttagcagtg attctaattg cagctgctct ttgatacaac taattttacg acgacgatgc
     4621 gagcttttat tcaaccgagc gtgcatgttt gcaatcgtgc aagcgttatc aatttttcat
     4681 tatcgtattg ttgcacatca acaggctgga caccacgttg aactcgccgc agttttgcgg
     4741 caagttggac ccgccgcgca tccaatgcaa actttccgac attctgttgc ctacgaacga
     4801 ttgattcttt gtccattgat cgaagcgagt gccttcgact ttttcgtgtc cagtgtggct
     4861 t
//
