LOCUS       62056_4040       14112 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14112)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2911..3582
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     rep_origin      complement(3489..4113)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     promoter        complement(4653..4683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4698..4719)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(5268..5520)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5913..6701)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6725..6908)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(7381..7405)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9004..10149)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10451..11242)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(13497..14107)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttgtgaacca tcacccaaat caagtttttt
     2521 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     2581 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     2641 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2701 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2761 cccagtcacg acgttgtaaa acgacggcca gtgaattgtt aattaagaat tcgagctcct
     2821 tgcatgcctg caggtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa
     2881 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg
     2941 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
     3001 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
     3061 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
     3121 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga
     3181 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
     3241 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
     3301 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
     3361 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     3421 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     3481 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     3541 cccttcctct atataaggaa gttcatttca tttggagagg acctcgagag tgaaccgtca
     3601 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     3661 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     3721 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     3781 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     3841 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     3901 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     3961 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4021 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4081 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4141 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4201 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4261 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4321 gacgagctgt acaaggtcga cggatcctct agatgaacta gagtccgcaa aaatcaccag
     4381 tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc
     4441 agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     4501 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     4561 aaaatccagt gacaagcttg gcgcgccagc ttggcgtaat catggtcata gctgtttcct
     4621 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     4681 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     4741 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4801 agaggcggtt tgcgtattgg gccaaagaca aaagggcgac attcaaccga ttgagggagg
     4861 gaaggtaaat attgacggaa attattcatt aaaggtgaat tatcaccgtc accgacttga
     4921 gccatttggg aattagagcc agcaaaatca ccagtagcac cattaccatt agcaaggccg
     4981 gaaacgtcac caatgaaacc atcgatagca gcaccgtaat cagtagcgac agaatcaagt
     5041 ttgcctttag cgtcagactg tagcgcgttt tcatcggcat tttcggtcat agccccctta
     5101 ttagcgtttg ccatcttttc ataatcaaaa tcaccggaac cagagccacc accggaaccg
     5161 cctccctcag agccgccacc ctcagaaccg ccaccctcag agccaccacc ctcagagccg
     5221 ccaccagaac caccaccaga gccgccgcca gcattgacag gaggcccgat ctagtaacat
     5281 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     5341 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     5401 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     5461 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     5521 ggggatcatc cgggtctgtg gcgggaactc cacgaaaata tccgaacgca gcaagatatc
     5581 gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg ccgttgatgt ggacgccggg
     5641 cccgatcata ttgtcgctca ggatcgtggc gttgtgcttg tcggccgttg ctgtcgtaat
     5701 gatatcggca ccttcgaccg cctgttccgc agagatcccg tgggcgaaga actccagcat
     5761 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     5821 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     5881 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     5941 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     6001 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     6061 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     6121 ttcggcaagc aggcatcgcc atgggtcacg acgagatcat cgccgtcggg catgcgcgcc
     6181 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     6241 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     6301 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     6361 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     6421 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     6481 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     6541 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     6601 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     6661 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatccagat
     6721 ccggtgcaga ttatttggat tgagagtgaa tatgagactc taattggata ccgaggggaa
     6781 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
     6841 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     6901 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     6961 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     7021 atcagattgt cgtttcccgc cttcagtttg tgggccatcg ccctgataga cggtttttcg
     7081 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7141 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggaacc
     7201 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     7261 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     7321 accaccccag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     7381 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     7441 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     7501 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     7561 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     7621 cagagcgttg ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc
     7681 ttcttattca tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat
     7741 aataggaaat cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt
     7801 gaacgttgac gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc
     7861 cgaagttccg actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga
     7921 gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga
     7981 agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg
     8041 taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg
     8101 ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca
     8161 gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat
     8221 ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc
     8281 ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca
     8341 tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa
     8401 taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc
     8461 accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg
     8521 cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg
     8581 acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa
     8641 tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg
     8701 cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct
     8761 tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa
     8821 tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg
     8881 cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc
     8941 ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc
     9001 ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc
     9061 tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc
     9121 cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct
     9181 gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa
     9241 tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca
     9301 acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc
     9361 caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca
     9421 ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc
     9481 gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg
     9541 ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac
     9601 gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt
     9661 gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg
     9721 ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt
     9781 cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt
     9841 tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc
     9901 cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg
     9961 gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc
    10021 gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc
    10081 atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt
    10141 ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta
    10201 ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa
    10261 gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg
    10321 cacgaatacc agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa
    10381 acacttgatg cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat
    10441 ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
    10501 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
    10561 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
    10621 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
    10681 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
    10741 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
    10801 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
    10861 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
    10921 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
    10981 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
    11041 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
    11101 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    11161 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    11221 gtgatattct cattttagcc atttattatt tccttcctct tttctacagt atttaaagat
    11281 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
    11341 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
    11401 atcgacggag ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag
    11461 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtg tctatcagct gtccctcctg
    11521 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct
    11581 ctgctctcac tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg
    11641 tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg
    11701 cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg
    11761 gtaacctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    11821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    11881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    11941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12121 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12181 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12241 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12301 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagtaac
    12361 ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg
    12421 ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca gtctccggaa
    12481 gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag
    12541 cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc
    12601 ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca
    12661 taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa
    12721 atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata
    12781 agttggagtc attacccaat tatgatagaa tttacaagct ataaggttat tgtcctgggt
    12841 ttcaagcatt agtccatgca agtttttatg ctttgcccat tctatagata tattgataag
    12901 cgcgctgcct atgccttgcc ccctgaaatc cttacatacg gcgatatctt ctatataaaa
    12961 gatatattat cttatcagta ttgtcaatat attcaaggca atctgcctcc tcatcctctt
    13021 catcctcttc gtcttggtag ctttttaaat atggcgcttc atagagtaat tctgtaaagg
    13081 tccaattctc gttttcatac ctcggtataa tcttacctat cacctcaaat ggttcgctgg
    13141 gtttatcgca cccccgaaca cgagcacggc acccgcgacc actatgccaa gaatgcccaa
    13201 ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg aagtgaaggg
    13261 caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga atgtcgatgc
    13321 cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg cccatcccgt
    13381 tactgccccg atcccggcaa tggcaaggac tgccagcgct gccatttttg gggtgaggcc
    13441 gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag cgggccggga
    13501 gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc
    13561 cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag acaggttagc
    13621 ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct gtggacagcc
    13681 cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca agtgtcaagg
    13741 atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc agggcactta
    13801 tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg ttttcgccga
    13861 tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca
    13921 acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga
    13981 gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc gcacacggct
    14041 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
    14101 caaccagccc gg
//
