LOCUS       62056_4250        6836 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      523..609
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(709..752)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(936..953)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(990..1706)
                     /codon_start=1
                     /label=SGFP2
                     /note="strongly enhanced green fluorescent protein (Kremers
                     et al., 2007)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQRNGIK
                     ANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     rep_origin      complement(2066..2654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2733..3590)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3591..3695)
                     /label=AmpR promoter
     CDS             4192..5193
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             5403..6776
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
ORIGIN
        1 agatctgcga ttgctgagta aaagatacga gagacctctc ttgtatcttt tttattttga
       61 gtggttttgt ccgttacact agaaaaccga aagacaataa aaattttatt cttgctgagt
      121 ctggctttcg gtaagctaga caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg
      181 gagatttttt gagtgatctt ctcaaaaaat actacctgtc ccttgctgat ttttaaacga
      241 gcacgagagc aaaacccccc tttgctgagg tggcagaggg caggtttttt tgtttctttt
      301 ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt ttggtcggca ctgccgacag
      361 cctcgcagag cacacacttt atgaatataa agtatagtgt gttatacttt acttggaagt
      421 gtacagaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaaggcc
      481 tacgtaccat tacagcggat caattcccaa ttccccaggc atcaaataaa acgaaaggct
      541 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      601 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
      661 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
      721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      781 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
      841 cgggcaggac gcccgccata aactgccagg aattggggat cggaattaat tttacaagcc
      901 cagagctcca ccgcggtggc ggccgctcta gattagtgat ggtgatggtg atgagaacct
      961 ctcgagcatg cggatccagc aaagctttat ttgtataatt catccatacc taaagtaata
     1021 ccagcagcag ttacgaattc taataatacc atgtgatcac gtttttcgtt tggatctttt
     1081 gataattttg attgagttga taagtagtgg ttatctggta ataatactgg accatcacca
     1141 attggagtgt tttgttggta gtggtaagct aattgtacac caccatcttc aatgttgtga
     1201 cgaattttga agttagcttt aataccgtta cgttgtttat cagccataat gtatacgttg
     1261 tgtgagttga agttgtattc taatttgtga cctaaaatgt taccatcttc tttgaaatca
     1321 atacctttta attcaatacg gtttactaaa gtatcacctt cgaattttac ttcagcacga
     1381 gttttgtagt taccatcatc tttgaagaaa atagtacgtt cttgaatgta accttctggc
     1441 atagctgatt tgaagaaatc gtgttgtttc atgtgatctg ggtaacgtga gaaacattgt
     1501 acaccgtaag ttaaagtagt tactaaagtt ggccatggta ctggtaattt accagtagta
     1561 caaatgaatt ttaaagttaa tttaccgtaa gtagcatcac cttcaccttc acctgatact
     1621 gagaatttgt gaccgtttac atcaccatct aattctacta aaattggtac tacaccagtg
     1681 aataattctt caccttttga taccatatga taaacctcct attttccttt cttgttttcc
     1741 attatatata atcccctgta tattttgcaa actctggtac cgcgttgcgc tcactgcccg
     1801 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1861 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1921 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1981 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2041 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2101 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2161 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2221 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2281 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2341 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2401 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2461 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2521 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2581 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2641 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2701 aaaactcacg ttaagggatt ttggtcatgt taccaatgct taatcagtga ggcacctatc
     2761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     3061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     3361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3661 tgtatttaga aaaataaaca aataggggtt ccgcggacat ttccccgaaa agtgccacct
     3721 gacgtctaag aaaccattat tatcatgaca ttaacctaga aagcactaag gaataattcc
     3781 ttaaggaacg tacagacgct taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa
     3841 ggtaaaggat aaaacagcac aattccaaga aaaacacgat ttagaaccta aaaagaacga
     3901 atttgaacta actcataacc gagaggtaaa aaaagaacga agtcgagatc agggaatgag
     3961 tttataaaat aaaaaaagca cctgaaaagg tgtctttttt tgatggtttt gaacttgttc
     4021 tttcttatct tgatacatat agaaataacg tcatttttat tttagttgct gaaaggtgcg
     4081 ttgaagtgtt ggtatgtatg tgttttaaag tattggaaaa cccttaaaat tggttgcaca
     4141 gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata gatgggggtt
     4201 tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc aagggtttta
     4261 gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt
     4321 gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt
     4381 gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg
     4441 tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat gaaacatggc
     4501 attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt tcgttggttg
     4561 tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa gagtttgtca
     4621 gatatggctc aaggatttcg ccgaatgatg caatataaaa aaattaataa aaatcttgtt
     4681 ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta taatcagcac
     4741 atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa ctacgtgaat
     4801 caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga tccaaatgta
     4861 aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc ggcaattgac
     4921 gaaactgcaa aatatcctgt aaaggatacg gattttatga ccgatgatga agaaaagaat
     4981 ttgaaacgtt tgtctgattt ggaggaaggt ttacaccgta aaaggttaat ctcctatggt
     5041 ggtttgttaa aagaaataca taaaaaatta aaccttgatg acacagaaga aggcgatttg
     5101 attcatacag atgatgacga aaaagccgat gaagatggat tttctattat tgcaatgtgg
     5161 aattgggaac ggaaaaatta ttttattaaa gagtagttca acaaacgggc catattgttg
     5221 tataagtgat gaaatactga atttaaaact tagtttatat gtggtaaaat gttttaatca
     5281 agtttaggag gaattaatta tgaagtgtaa tgaataatga atgtaacagg gttcaattaa
     5341 aagagggaag cgtatcatta accctataaa ctacgtctgc cctcattatt ggagggtgaa
     5401 atgtgaatac atcctattca caatcgaatt tacgacacaa ccaaatttta atttggcttt
     5461 gcattttatc tttttttagc gtattaaatg aaatggtttt gaacgtctca ttacctgata
     5521 ttgcaaatga ttttaataaa ccacctgcga gtacaaactg ggtgaacaca gcctttatgt
     5581 taaccttttc cattggaaca gctgtatatg gaaagctatc tgatcaatta ggcatcaaaa
     5641 ggttactcct atttggaatt ataataaatt gtttcgggtc ggtaattggg tttgttggcc
     5701 attctttctt ttccttactt attatggctc gttttattca aggggctggt gcagctgcat
     5761 ttccagcact cgtaatggtt gtagttgcgc gctatattcc aaaggaaaat aggggtaaag
     5821 catttggtct tattggatcg atagtagcca tgggagaagg agtcggtcca gcgattggtg
     5881 gaatgatagc ccattatatt cattggtcct atcttctact cattcctatg ataacaatta
     5941 tcactgttcc gtttcttatg aaattattaa agaaagaagt aaggataaaa ggtcattttg
     6001 atatcaaagg aattatacta atgtctgtag gcattgtatt ttttatgttg tttacaacat
     6061 catatagcat ttcttttctt atcgttagcg tgctgtcatt cctgatattt gtaaaacata
     6121 tcaggaaagt aacagatcct tttgttgatc ccggattagg gaaaaatata ccttttatga
     6181 ttggagttct ttgtggggga attatatttg gaacagtagc agggtttgtc tctatggttc
     6241 cttatatgat gaaagatgtt caccagctaa gtactgccga aatcggaagt gtaattattt
     6301 tccctggaac aatgagtgtc attattttcg gctacattgg tgggatactt gttgatagaa
     6361 gaggtccttt atacgtgtta aacatcggag ttacatttct ttctgttagc tttttaactg
     6421 cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag
     6481 gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc
     6541 aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag
     6601 gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta
     6661 tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca
     6721 ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa
     6781 tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta taacag
//
